This collection of discussions reveals widespread acknowledgment of the scientific milestone achieved in synthesizing infectious polioviruses from genetic sequences, with users emphasizing the significance of creating viruses from scratch and confirming their infectivity through genetic and experimental evidence. The tone ranges from factual reporting to concern over bioethical implications, illustrating both interest and recognition of the potential impact on fields like public health and biosecurity.
AgreementStrong agreement
Most discussions strongly support the significance and reality of synthesizing infectious polioviruses from scratch, confirming the scientific achievement.
InterestHigh interest
Posts demonstrate high curiosity and engagement, often referencing detailed methods, historical context, and implications for science and society.
EngagementHigh engagement
Participants cite specific papers, share examples of lab results, and debate the safety and ethics, showing deep involvement.
ImpactHigh impact
These discussions highlight a groundbreaking development with profound implications for biotechnology, virology, and biosecurity.
Abstract in brief
A full-length poliovirus cDNA was chemically synthesized by assembling oligonucleotides of both plus and minus strands, which was then transcribed into viral RNA.
The synthetic viral RNA was able to translate and replicate in a cell-free system, leading to the creation of infectious poliovirus without using a natural template.
Tests in tissue culture and transgenic mice confirmed that the lab-made virus exhibited biochemical properties and caused disease similar to natural poliovirus, demonstrating the feasibility of creating infectious agents from written genetic sequences alone.]
Day 4 of synthetic biology papers.
“Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template” (2002)
“Refactoring bacteriophage T7” (2005)
The early days of genome synthesis. Why tweak organisms when you can build them anew?
*** https://t.co/7eubcp1pCV
@PatriceRoyTJ Vraiment bizarre. Ça fait un bon boutte qu'on fabrique des virus synthétiques. Il n'est pas au courant, le Dr. Weiss?
Ça fait au dessus de 20 ans qu'on fabrique des virus 100% synthétiques.
https://t.co/b9tTzK0Hqi. https://t.co/Tz97oVMcg8
Mar 13, 2023 · 13 likes · 2 shares · 604 followersView post →
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AdrianGibbs1934
@AdrianJ44426152 · X
@WashburneAlex Cello, J., Paul, A.V. and Wimmer, E., 2002. Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template. science, 297(5583), pp.1016-1018.
Mar 4, 2023 · 12 likes · 1 share · 413 followersView post →
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Maryam Henein/ (aka BeeLady) / Non Psyop
@MaryamHenein · X
Nov 16, 2023 · 8 likes · 4 shares · 25,800 followersView post →
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Sanjay "John"
@sanjay_world · X
Example publications (there are many)
https://t.co/FrSd0DP84K
and
https://t.co/MogOY4idXG
Mar 23, 2023 · 7 likes · 2 shares · 8,201 followersView post →
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Mohamed ElTanbouly
@Plasmacellguy · X
20- Chemical synthesis of a virus
Eckard Wimmer and colleagues describe the synthesis of poliovirus in vitro (cell-free system)
Year: 2002
Paper ➡️ https://t.co/3dmtVHEXqZ https://t.co/xVHN4XeLG2
The Resident Evil universe involves the development of the T-Virus by Umbrella, transforming humans into zombies. This video explores how realistic such viruses are, including the possibility of creating and modifying…
This video explores the chemical synthesis of poliovirus cDNA, which led to the generation of infectious virus without a natural template, demonstrating how synthetic viral RNA can replicate and cause disease similar to…
RT @NikoMcCarty: Day 4 of synthetic biology papers.
“Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence…
Mar 25, 2025 · 49 shares · 38 followersView post →
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Mike Famulare
@famulare_mike · X
Exact chemical formula for a poliovirus.
https://t.co/Z9SuY44w0U https://t.co/uOM4x1DYG7 https://t.co/pz5IksTQAy
Mar 14, 2025 · 2 likes · 3,274 followersView post →
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Desmosome
@Desmosome1 · X
@BdGEORGES Chemical synthesis of poliovirus cDNA: Generation of infectious virus in the absence of natural template. Nature (2002). https://t.co/Lrf6T6vs1h
وزارة الصحة الفلسطينية تعلن تفش فيروس شلل الاطفال فى مياة غزة .
وهل هو سلاح بيولوجى لابادة الشعب فى غزة؟
انظر مقالة تصنيع الفيروس .
Chemical synthesis of poliovirus cDNA: Generation of infectious virus in the absence of natural template. Nature (2002). https://t.co/Lrf6T6vs1h
@PhotoMakina So you're saying that to be isolated, an obligate intracellular parasite is not allowed to be cultured in cells?
If you really want, you can use cell free culture, or you could use primary human cells and organoids.
https://t.co/nwrjfav6Qe
Nov 17, 2023 · 4 shares · 505 followersView post →
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Hank Pirrone
@HankPirrone · X
RT @MaryamHenein:
Nov 17, 2023 · 4 shares · 226 followersView post →
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TiamoAugustusjupiter
@tiamoCAESAR · X
RT @MaryamHenein:
Nov 16, 2023 · 4 shares · 247 followersView post →
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Courtenay Turner
@CourtenayTurner · X
RT @MaryamHenein:
Nov 16, 2023 · 4 shares · 8,194 followersView post →
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Maryam Henein/ (aka BeeLady) / Non Psyop
@MaryamHenein · X
Nov 16, 2023 · 8 likes · 4 shares · 25,800 followersView post →
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Infinituple
@Infinituple · X
20 years ago poliovirus was synthesised from scratch, proving that a virus can be reproduced without access to the original.
This had implications for reproduction of other viruses as well especially given the free availability of genomes.
https://t.co/BQJhINb3fE
@alrightyman @JesperKitos @ThomMohr @zwergie1 @Tetrahydropyran @hauxton @ImAtSeaAgain @PrimalDaoist @SynthIge @TakethatCt @arrdeearr @JRJHealey @PenOfAPisces @niswarth @JimeeLiberty @DocDeezWhat @ThroneOfCrypto @sunshine_toes @DiscernHeal @DdkDav @HamHead5813 @PChandr43659403 @canceledmouse @ScooterScience @SamolejJ @RightHandWatch @rw_christian @BoyScou84038220 @sanjay_world @ViroLIEgy @dan_gadd @1984rebooted @CarlSmytheCells @dirk_fasshauer @macgyvertang20 @paramaniac9 @MichelleZeh1968 @dave59_craft @kevinoler @NamelessGhoulTX @virologytruth @ValZimmer2 @Pablosquest @zbingledack @TrevorJukes1 @catsequalneko @sense_strand @Candles4Assange That's commonly claimed by virus deniers, but it simply isn't true. There is an overwhelmingly large amount of evidence for the existence of viruses. Just one example, polioviruses have even been made in the lab from scratch, using the published genome
@RandolphCarterZ @PessimistsArc @AndrewYang Also worth noting that making viruses using chemically synthesized genome is not new science, and that I haven't seen much skepticism from the biological community around this specific paper, so I'm inclined to believe it's real.
See:
https://t.co/1FmmIpyz93
Aug 12, 2023 · 1 like · 1,431 followersView post →
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Ave Maria, PhD
@AveMari86640667 · X
RT @drloveariyana: @Inversionism This new frontier of synthetic biology began with the synthesis of the Polio pathogen.
https://t.co/wlFs…
Aug 11, 2023 · 2 shares · 1,326 followersView post →
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Dr. Ariyana Love
@drloveariyana · X
@Inversionism This new frontier of synthetic biology began with the synthesis of the Polio pathogen.
https://t.co/wlFsZxfBP1
Also see:
https://t.co/EEXHu7JASg
Aug 11, 2023 · 5 likes · 2 shares · 4,631 followersView post →
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Paul Holloway
@pjholloway · X
@DocDeezWhat @JimeeLiberty @ProtocolosA @JRJHealey @HamHead5813 @MonetName @CarlSmytheCells @DiscernHeal @dan_gadd @hauxton @PChandr43659403 @Tetrahydropyran @ViroLIEgy @zwergie1 @ThomMohr @1984rebooted @ThroneOfCrypto @ScooterScience @DdkDav @MichelleZeh1968 @dave59_craft @PenOfAPisces @kevinoler @NamelessGhoulTX @virologytruth @ValZimmer2 @dirk_fasshauer @Pablosquest @arrdeearr @TakethatCt @macgyvertang20 @zbingledack @TrevorJukes1 @BoyScou84038220 @sanjay_world @JesperKitos @catsequalneko @sunshine_toes @sense_strand @Candles4Assange @Alec_Zeck @McSheree3 @LilElectronBlue @rw_christian @mauveTriforce @thisisnothappen @Zignack @JCmacc1 @mitrouscience @Bio_inspiration Scientists have made polioviruses from scratch, and they paralysed mice. Even that doesn't appear to convince them.
https://t.co/MGb5eq038Y
@BoyScou84038220 @sunshine_toes @DiscernHeal @DocDeezWhat @sense_strand @ThomMohr @Candles4Assange @CarlSmytheCells @Tetrahydropyran @PChandr43659403 @macgyvertang20 @DdkDav @dan_gadd @Alec_Zeck @JesperKitos @hauxton @arrdeearr @McSheree3 @HamHead5813 @PenOfAPisces @ViroLIEgy @dave59_craft @LilElectronBlue @JRJHealey @ThroneOfCrypto @TakethatCt @MonetName @rw_christian @mauveTriforce @dirk_fasshauer @thisisnothappen @ValZimmer2 @Zignack @JCmacc1 @mitrouscience @Bio_inspiration @Everybody5Dad @PetrusMax4 @controscience @andyparksmma @PeaceLillie33 @MissytheMuse @The_Seeadler @GaryOxide @claushetting @DrRobertWMarlo1 @Dammadara @SirMasksALot @KStateTurk @SwaledaleMutton Was the poliovirus made from scratch by scientists, using the published genome, a figment of their imaginations? How did a figment of their imaginations paralyze mice?
https://t.co/MGb5eq038Y
Jul 27, 2023 · 1 like · 1 share · 123 followersView post →
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José Luis Duarte de Jesús
@JosLuisDuarted1 · X
RT @NikoMcCarty: Day 4 of synthetic biology papers.
“Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence…
Day 4 of synthetic biology papers.
“Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template” (2002)
“Refactoring bacteriophage T7” (2005)
The early days of genome synthesis. Why tweak organisms when you can build them anew?
*** https://t.co/7eubcp1pCV
@NicoleTenev @ggronvall @thackerpd @T_Inglesby I'd say 2002, when a scientist synthesized the polio virus from scratch - although that's not quite the same as gain-of-function. It caused a huge outcry though, and it did lead to new self-regulating policies at scientific journals: https://t.co/kIk3mnMI5D
May 27, 2023 · 1 like · 19,293 followersView post →
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giróvago
@girovago111 · X
RT @sanjay_world: Example publications (there are many)
https://t.co/FrSd0DP84K
and
https://t.co/MogOY4idXG
Mar 23, 2023 · 2 shares · 326 followersView post →
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Sanjay "John"
@sanjay_world · X
Example publications (there are many)
https://t.co/FrSd0DP84K
and
https://t.co/MogOY4idXG
Mar 23, 2023 · 7 likes · 2 shares · 8,201 followersView post →
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Paul Holloway
@pjholloway · X
@EyesOpen129 @sanjay_world Are you aware that scientists made polioviruses, from scratch, using the published genome? These artificially made viruses infected mice, paralyzing them, could be isolated from those mice & looked identical to regular polioviruses under EM. Explain that.
https://t.co/MGb5eq038Y
@IsWorldChanging @laotracara88 @3dom13 Did you know scientists made polioviruses, from scratch, using the published genome. They were just like wild polioviruses, and when the researchers injected these viruses into mice, the mice became paralyzed.
https://t.co/MGb5eq038Y
RT @Lee98604032: @PatriceRoyTJ Vraiment bizarre. Ça fait un bon boutte qu'on fabrique des virus synthétiques. Il n'est pas au courant, le…
Mar 14, 2023 · 2 shares · 208 followersView post →
?
⚜️
@VoisinSt · X
RT @Lee98604032: @PatriceRoyTJ Vraiment bizarre. Ça fait un bon boutte qu'on fabrique des virus synthétiques. Il n'est pas au courant, le…
Mar 14, 2023 · 2 shares · 1,322 followersView post →
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Lee
@Lee98604032 · X
@PatriceRoyTJ Vraiment bizarre. Ça fait un bon boutte qu'on fabrique des virus synthétiques. Il n'est pas au courant, le Dr. Weiss?
Ça fait au dessus de 20 ans qu'on fabrique des virus 100% synthétiques.
https://t.co/b9tTzK0Hqi. https://t.co/Tz97oVMcg8
Mar 13, 2023 · 13 likes · 2 shares · 604 followersView post →
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AdrianGibbs1934
@AdrianJ44426152 · X
@WashburneAlex Cello, J., Paul, A.V. and Wimmer, E., 2002. Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template. science, 297(5583), pp.1016-1018.
Mar 4, 2023 · 12 likes · 1 share · 413 followersView post →
@jrmy_tu @Alex_on_A14 @ericmichael74 @Mr_Magoo5 @JonathanHannah @reacharoundu @AndrewLazarus4 @LifeAfterWEF @BreezerGalway @GenderBreadMann @CaeJak @Spoonhead8 @old_move @carlsmythe @Jaytee116James @tarsins @richykirsh @andylumm @ttroutma @mpc_xetts @djchicus @ghoppe @JimeeLiberty @Tarsins1 @PremierImproves @Turrialba27 @Willard1951 @FillmoreWhite @SkepticalMutant @doom37455413 @JamesLynchGTC @anxiety_korma @Amber84280581 @TonyBaduy @MsTrixter @WalterTAllen @Dominos20076133 @AlbertRodz5 @FoolanderWorld @chimera414 @FvckYourFear @fadgesniffer @nuhope2022 @ShockTraumaRN @paramaniac9 @Theonew16516042 @expose_the_dog @mcfunny @lazlowoodbine42 @malo_j Yes, they produced infective polioviruses.
You can buy the paper here:
https://t.co/FEE3x7ghhC
The author discusses his work here:
https://t.co/TtgiggKbgq
Jul 27, 2022 · 1 like · 1 share · 951 followersView post →
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Krebiozen
@krebiozen · X
@FrankDElia7 @Whitey900 @carlsmythe @TakethatCt @richykirsh @robertopaulos85 @Kathmarval @LiamKav @CallMeEOnly @Top_Muppetries @ghoppe @mcfunny @_mamadeb @TonyBaduy @SkepticalMutant @DevoDalek @ncdave4life @Monstercoyliar @CharlieGordon20 @crabb_vicki @kanwee9 @EstesPga @slemar_ @AGoldsmithEsq @AndrewLazarus4 @DCGreenZone1 @CoralBlob @quigley_jesse @wendy_Lynnette @clownworld_bobo @ZaynJaffer @provaxtexan @Mchael21592783M @JustPlaying2Win @tenebra99 @i_am_joli_anne @gobantwo2 @melissa94307909 @doritmi @stevenmosher @JawKneeFaiv @MickusDickus @policing_uk @BrockKrause @dodi_rains @RenfieldThomas @indyymac @Kolyin I've showed germ denialists how scientists have created polioviruses from scratch, using the genome published on the internet, and the mice they infected became paralyzed. They claim the mice were paralyzed by toxins in the nutrient solution, or something.
https://t.co/El8zHyrogX
Co-signed by James LeDuc, quoted in Daily Mail (07/2002) article (re https://t.co/ks42l9onf9 "Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template) as saying 'a little sobering to see that folks can create a virus from scratch'. https://t.co/QHX8DGLtaZ
RT @FillmoreWhite: @JohnPapasIdeas @GarretKramer Polio viruses have been isolated, sequenced, and synthesized.
https://t.co/lYLljnEtF7
Mar 4, 2022 · 1 share · 2,756 followersView post →
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Millard Fillmore White House Library
@FillmoreWhite · X
@JohnPapasIdeas @GarretKramer Polio viruses have been isolated, sequenced, and synthesized.
https://t.co/lYLljnEtF7
Mar 3, 2022 · 1 like · 1 share · 2,756 followersView post →
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Jorgelina
@JorgelinaSanto5 · X
RT @Charlos79: @SanmartinianoAr @iayala593 Se las inventan...
En teoria pueden crear, reproducir o replicar cualquier virus...
En 2002…
Jan 11, 2022 · 1 share · 1,356 followersView post →
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T'Charlos
@Charlos79 · X
@SanmartinianoAr @iayala593 Se las inventan...
En teoria pueden crear, reproducir o replicar cualquier virus...
En 2002 lo hicieron por primera vez con la polio...
https://t.co/fz4q2eGVwd
Jan 10, 2022 · 6 likes · 1 share · 275 followersView post →
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Mohamed ElTanbouly
@Plasmacellguy · X
20- Chemical synthesis of a virus
Eckard Wimmer and colleagues describe the synthesis of poliovirus in vitro (cell-free system)
Year: 2002
Paper ➡️ https://t.co/3dmtVHEXqZ https://t.co/xVHN4XeLG2
Nothing is new under the sun. Synthetic poliovirus from 2002
Feb 20, 2021 · 2 likes · 1 share · 1,150 followersView post →
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Krebiozen
@krebiozen · X
@dbab1981 @BMcB77437937 @PaulVCooper1 @Christi45657364 @luciandipeso @RisetteMD @1_TMF_ @emartinez78987 @provaxtexan @mcfunny @Bluedawg19 @GeoffSchuler @JulieHouseOfWow @Bobby_Network @doritmi @AndrewLazarus4 @ianfmusgrave @swedishchf @Ephesians6__12 @7_DSSEVEN @KStateTurk @FrankDElia7 @TinFoilAwards @SkepticalMutant @jkellyca @pnpeterman @Just4TheCause @MsTrixter @Shared_facts @ArumughamVinu @crabb_vicki @DreadPirRob42 @handmadekathy @PaulCaw05364381 @flitesurgn @SBronisz @Charitable_Fury @LesleyStock5 @truthhealeruk @md444444444 @KSbyAuntieNic @ascendingdove11 @paulmuaddib611 @margie_moo @sQuishChick_Oz @thefoxygoddess @Bloom13132 @paulmuaddib61 @ale_battini @lysandergrant They claim those are just exosomes, or something.
Scientists have created polioviruses from scratch and injected them into mice which then became paralyzed. That seems pretty conclusive to me!
https://t.co/aJwfRoMva
@BMcB77437937 @KStateTurk @PaulVCooper1 @Christi45657364 @luciandipeso @RisetteMD @1_TMF_ @emartinez78987 @provaxtexan @mcfunny @Bluedawg19 @GeoffSchuler @JulieHouseOfWow @Bobby_Network @doritmi @AndrewLazarus4 @ianfmusgrave @swedishchf @Ephesians6__12 @7_DSSEVEN @FrankDElia7 @TinFoilAwards @SkepticalMutant @jkellyca @pnpeterman @Just4TheCause @MsTrixter @Shared_facts @ArumughamVinu @crabb_vicki @DreadPirRob42 @handmadekathy @PaulCaw05364381 @flitesurgn @SBronisz @Charitable_Fury @LesleyStock5 @truthhealeruk @md444444444 @KSbyAuntieNic @ascendingdove11 @paulmuaddib611 @margie_moo @sQuishChick_Oz @thefoxygoddess @Bloom13132 @paulmuaddib61 @ale_battini @lysandergrant Viruses have been isolated, imaged using EM, genotyped, and even created from scratch and used to infect animals.
https://t.co/aJwfRoMvaN
We know how they infect cells, and much more about them. Claiming they don't exist is ju
@BlainEillimatta @GeoffSchuler @emartinez78987 @PaulVCooper1 @mcfunny @FransPietersma @SkepticalMutant @HippieHeathen1 @FrankDElia7 @provaxtexan @CJBowden1 @Bobby_Network @xrptlrsem @dbab1981 @LG28640297 @PaulCaw05364381 @AndrewLazarus4 @TravelLightP1 @swedishchf @Ephesians6__12 @7_DSSEVEN @KStateTurk @Bluedawg19 @TinFoilAwards @jkellyca @pnpeterman @Just4TheCause @RisetteMD @MsTrixter @Shared_facts @ArumughamVinu @crabb_vicki @DreadPirRob42 @handmadekathy @flitesurgn @SBronisz @doritmi @HMEM11 @Charitable_Fury @LesleyStock5 @truthhealeruk @md444444444 @KSbyAuntieNic @ascendingdove11 @paulmuaddib611 @margie_moo @sQuishChick_Oz @thefoxygoddess @Bloom13132 @paulmuaddib61 A poliovirus has been assembled from scratch using its genome published on the internet, its presence confirmed by various means and then used to infect mice which developed flaccid paralysis. That seems pretty conclusive
Cuando la posibilidad de sintetizar un agente infeccioso únicamente siguiendo instrucciones de una secuencia escrita pasa de la teoría a la práctica:
https://t.co/mr4EDs7lSA
Science 297: 10161018. doi:10.1126/science.1072266. PMID 12114528. [53] Wimmer, E.; Mueller, S.; Tumpey, T. M.; Taubenberger, J. K. (2009). Synthetic ...
Biological Warfare - The History of Microbial Pathogens | PDF ...
News
Science 297, 1016–1018. doi:10.1126/science.1072266. Conclusion 14. Bjørn Jensen, L. et al. (2018) The delivery of health promotion and environ- mental ...
Diffusion of Synthetic Biology A Challenge To Biosafety | PDF ...
News
Science 297(5583):10161018. doi:10.1126/science. Klotz L (2007) Casting a wider net for countermeasure R&D funding 1072266 decisions. Biosecurity and ...
Teremtés a semmiből: az első mesterséges vírus - ORIGO
News
Science published online, doi: 10.1126/science.1072266 - 2002). Ajánlat: State University of New York in Stony Brook · Google News A legfrissebb hírekért ...
Jeronimo Cello et al. Science 297, 1016 (2002); DOI: 10.1126/science.1072266. This copy is for your personal, non-commercial use only. If you wish to ...
Chemical Synthesis of Poliovirus cDNA: Generation of Infectious ...
News
... 10.1126/science.1072266. Full-length poliovirus cDNA was synthesized by assembling oligonucleotides of plus and minus strand polarity. The synthetic ...
Chemical Synthesis of Poliovirus cDNA: Generation of Infectious ...
News
Science. 11 Jul 2002. Vol 297, Issue 5583. pp. 1016-1018. DOI: 10.1126/science.1072266 · PREVIOUS ARTICLE. Microbial Reefs in the Black Sea Fueled by Anaerobic ...
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