Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.

Abstract in brief

• This text discusses the development of improved monomeric fluorescent proteins from the Discosoma sp red fluorescent protein, aimed at reducing toxicity and disruption compared to earlier tetrameric versions.
• The scientists created a new generation of fluorescent proteins, including a more stable red version that matures faster, tolerates fusions better, and is over ten times more photostable than the previous mRFP1.
• These advances include three new monomers with different hues from yellow-orange to red-orange, all with higher quantum efficiencies, expanding the usefulness of fluorescent proteins in biological research.]