PubHawk
Protein Sci November 2016 PMID 27515297

Side effects of extra tRNA supplied in a typical bacterial protein production scenario.

Karina Marie Søgaard, Morten H H Nørholm

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Mentions

106
Peak: 10 in Dec 2025
across 7 sources since May 2017

Extended reach

33,785
potential audience

Total likes

69

Video views

3,341

Mentions per month

May 2017 – Jan 2026

Where it's discussed

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AI synopsis

What people are saying

The group expresses a fair level of agreement with the publication, with discussions pointing to the complexity of bacterial expression systems, such as mentions of plasmid antibiotic resistance and specific strains. The tone reflects curiosity and technical engagement, indicating that the findings impact researchers' understanding rather than immediate application.

AgreementModerate agreement

Most discussions recognize the importance of the findings, with some noting additional factors like plasmid characteristics and broader implications, reflecting a general agreement with the publication's significance.

InterestModerate interest

Participants show moderate interest, referencing specific strains, plasmids, and related research, indicating a curiosity about the nuances of bacterial protein production.

EngagementModerate engagement

The comments include technical details, such as plasmid types and gene expression mechanisms, demonstrating a moderate to high level of engagement and familiarity with molecular biology concepts.

ImpactNeutral impact

The discussions suggest the findings could influence experimental design and understanding of bacterial expression systems, but no comments indicate immediate or widespread practical change.

Abstract in brief

  • Supplying extra tRNA using the pLysSRARE2 plasmid in bacterial protein production can negatively impact bacterial growth and gene expression.
  • Some observed positive effects attributed to extra tRNA may actually be due to differential expression of the lysozyme gene on the same plasmid, not the tRNAs themselves.
  • These side effects and potential misunderstandings have been largely overlooked despite the widespread use of T7pET-based systems in biotechnology.]
Read the full abstract

Top posts

Most liked on X and Bluesky
Kresten Lindorff-Larsen
@LindorffLarsen · X

@DrJamesTorpey @kamilgoretsky You might enjoy this paper from @norholm_m Side effects of extra tRNA supplied in a typical bacterial protein production scenario https://t.co/Q8WRlqQsaG

May 21, 2022 · 5 likes · 14,711 followers View post →
Kresten Lindorff-Larsen
@LindorffLarsen · X

@klausenhauser There’s more to pLysSRARE2 than tRNA as shown in this work from @norholm_m Side effects of extra tRNA supplied in a typical bacterial protein production scenario https://t.co/Q8WRlqQsaG

Feb 5, 2023 · 2 likes · 14,711 followers View post →
The bumbling biochemist (Bri Bibel)
@bumblingbiochemist.bsky.social · Bluesky

Does the plasmid you ordered have 2 antibiotics listed? One might actually be for cells that plasmid is in (e.g. BL21(DE3)-RIL) which themselves have a plasmid, containing genes for tRNAs for rare codons… youtu.be/uUFuyu0gQ9k

Dec 2, 2025 · 1 like · 1 share View post →
Invictus Abu (Nicholas Phiri-Jaschke - Yahusha)
@AbuInvictus · X

Bacterial expression strains - esp BL21 strains with plasmids containing tRNAs for rare codons, https://t.co/b1WNmDZSBV

Nov 7, 2022 · 21 followers View post →

All mentions

The bumbling biochemist (Bri Bibel)
@bumblingbiochemist.bsky.social · Bluesky

Does the plasmid you ordered have 2 antibiotics listed? One might actually be for cells that plasmid is in (e.g. BL21(DE3)-RIL) which themselves have a plasmid, containing genes for tRNAs for rare codons… youtu.be/uUFuyu0gQ9k

Dec 2, 2025 · 1 like · 1 share View post →
Kresten Lindorff-Larsen
@LindorffLarsen · X

@klausenhauser There’s more to pLysSRARE2 than tRNA as shown in this work from @norholm_m Side effects of extra tRNA supplied in a typical bacterial protein production scenario https://t.co/Q8WRlqQsaG

Feb 5, 2023 · 2 likes · 14,711 followers View post →
Invictus Abu (Nicholas Phiri-Jaschke - Yahusha)
@AbuInvictus · X

Bacterial expression strains - esp BL21 strains with plasmids containing tRNAs for rare codons, https://t.co/b1WNmDZSBV

Nov 7, 2022 · 21 followers View post →
Kresten Lindorff-Larsen
@LindorffLarsen · X

@DrJamesTorpey @kamilgoretsky You might enjoy this paper from @norholm_m Side effects of extra tRNA supplied in a typical bacterial protein production scenario https://t.co/Q8WRlqQsaG

May 21, 2022 · 5 likes · 14,711 followers View post →
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Cui L, Guo Q, Wang X, Duffy KJ, Dai X (2021) Midgut bacterial ...
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DOI: 10.1002/prot.26142 . Martel V., Thireau J.C., Régnière J ... pro- and mesotibiae of male very weakly widened to apex, tarsi not ...

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Language conflict in DRC with English learning implications
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Birds are classified as reptiles in modern cladistic systems
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PDF. Motani, R. and Vermeij, G.J. (2021/in press), Ecophysiological ...
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According to Maclver ''conceptual" what's the basis of social ...
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Hello everyone! I am looking for some references that I can´t find ...
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Por que piscamos muito? O que significa? | TikTok
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https://doi.org/10.1002/jip.150 Hirschberg, J., & Mitchell, S. (2019) ... pro meu canal e dormia mau e pouco! E vc sabe mais qual outro ...

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Maximum diversity of reptiles was during??? a) Jurassic b ...
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A Hearty Congratulations to the Caribbean Heart Care Medcorp ...
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3,011 noncardiac surgical procedures 2008 to 2014 Median age 4 ... https://onlinelibrary.wiley.com/doi/10.1002/ejhf. 3388?fbclid ...

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Atopodentatus, a genus of marine reptile from middle Triassic China ...
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Which of the following is not true about the Taiga Forests? (A) The ...
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... (3,011 m). * Maximum N->S distance: 2,500 km. * Maximum E->W distance ... https://esajournals.onlinelibrary.wiley. com/doi/abs/10.1002/eap.

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Good resource from Harvard.
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What is the application of impulse & momentam
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Why are reptiles more advance than amphibians in evolutionary ...
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I've coauthored with Prof. Mu-Hyun (Mookie) Baik, a tutorial of ...
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... 10.1002/jcc. 26758](https://onlinelibrary.wiley. com/doi/10.1002/jcc ... Buying: EMA 3011: Physical Chemistry: A Molecular Approach ...

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Today chemisrty is used in every walk of life, some passionate ...
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What is the diversity of crocodilian forms?
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My 13 year old son Daniel died in August from osteosarcoma. When ...
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