Genome Biol February 2017 PMID 28143571

Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects.

Yuanpeng Gao, Haibo Wu, Yongsheng Wang, Xin Liu, Linlin Chen, Qian Li, Chenchen Cui, Xu Liu, Jingcheng Zhang, Yong Zhang

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Peak: 2 in Aug 2019

across 6 sources since Feb 2017

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Feb 2017 – Oct 2019

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AI synopsis

What people are saying

The discussions emphasize the significance of using precise gene editing tools to develop resilient cattle, with examples such as mentions of 'CRISPR' and 'TB resistant cows.' Words like 'interesting' and phrases like 'use of CRISPR' demonstrate curiosity and positive evaluations of the research's implications for both scientific progress and practical applications.

AgreementModerate agreement

Most discussions express support or approval for the research, highlighting its potential benefits.

InterestHigh interest

Participants show high curiosity, focusing on innovative techniques like CRISPR and their applications in disease resistance.

EngagementModerate engagement

The comments reflect active engagement, referencing the methods used and implications for agriculture and medicine.

ImpactModerate impact

Thoughts indicate that the research is seen as impactful, potentially advancing gene editing and combating antimicrobial resistance.

Abstract in brief

This study is about using a special gene editing tool called Cas9 nickase (Cas9n) to insert the NRAMP1 gene into cattle, which helps make them more resistant to tuberculosis, while reducing unwanted off-target effects compared to other methods.
The researchers first identified where the inactive form of Cas9 binds in bovine cells and then used Cas9n to make precise single-strand cuts, enabling the targeted insertion of the NRAMP1 gene in cattle cells.
The final outcome was successful production of transgenic cattle through cloning techniques, showing improved disease resistance, and demonstrating that this approach could be useful for agricultural improvements with fewer genetic editing mistakes.]

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All mentions

Advances in CRISPR-Based Gene Editing for Disease Resistance in Cattle

CRISPR gene editing technology is transforming how we enhance cattle disease resistance by using precise tools like Cas9 nickase to insert beneficial genes with fewer off-target effects, leading to improved livestock…

Hashem Al-Ghaili · Aug 8, 2017 16,013 views

CRISPR Advances in Genetic Editing and Ethical Considerations

This episode discusses recent CRISPR applications in plants, animals, and human health, highlighting ongoing advancements and ethical issues surrounding genetic modification, including bioterrorism and GMOs.

PETRI DISH · Oct 21, 2019 51 views

@Bios22B · X

RT @BinMohammed07: Refs; https://t.co/xMCdef5EHp https://t.co/9bol3biF4v https://t.co/VdQ48uq69l https://t.co/FvKcx3aUnj https://t.co…

Aug 13, 2019 · 1 share · 374 followers View post →

ALI MUHAMMED ~

@BinMohammed07 · X

Aug 13, 2019 · 6 likes · 1 share · 2,319 followers View post →

Brigitte Nerlich

@bnerlich.bsky.social · Bluesky

Use of #CRISPR to make TB resistant cows. Interesting from both a #synbio and #AMR perspective http://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-1144-4

Feb 1, 2017 View post →