Neuron November 2017 PMID 29056297

Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain.

Jun Nishiyama, Takayasu Mikuni, Ryohei Yasuda

Mentions

28
Peak: 3 in Jan 2024
across 5 sources since Oct 2017

Extended reach

100,074
potential audience

Total likes

207

Video views

21,951

Mentions per month

Oct 2017 – Dec 2025

Where it's discussed

  • X / Twitter11 posts
  • News8 articles
  • Facebook5 posts
  • YouTube2 videos
  • TikTok2 posts

AI synopsis

What people are saying

The overall tone balances scientific curiosity with skepticism, illustrated by comments like 'Cas9 project crispr. Lekker blijven prikken' and mentions of the technique's versatility, such as tagging proteins in the brain. The use of informal language and references to further research, including RRIDs and reproducibility, reflect both engagement with technical details and cautious optimism about its significance.

AgreementNeither agree nor disagree

The posts display mixed reactions, with some expressing curiosity and others being skeptical or dismissive of the technique's implications.

InterestModerate interest

There is a moderate level of interest, with users showing curiosity about the research and contemplating applications beyond neuroscience.

EngagementModerate engagement

Discussions include references to methods, such as the use of CRISPR-Cas9 and AAV, and mention practical considerations, indicating some depth of engagement.

ImpactModerate impact

The social discussions highlight potential impact in research and medicine, though some posts focus on the technological novelty rather than broader societal effects.

Abstract in brief

  • This study demonstrates that homology-directed repair (HDR) can be effectively used for precise genome editing not only in dividing cells but also in mature, non-dividing neurons in the mouse brain by combining CRISPR-Cas9 with adeno-associated virus (AAV) for delivery.
  • The researchers successfully tagged endogenous proteins in various brain cell types both in vitro (cell culture and brain slices) and in vivo (developing, adult, and diseased brains), highlighting the technique's versatility.
  • This advancement suggests that AAV and CRISPR-Cas9 mediated HDR could become valuable tools for detailed genetic modifications in neuroscience research, including studies on brain function and disease.]

Top posts

Most liked on X and Bluesky
Neuroweapon Forensics
@neuroweapons · X

This tool allows a virus to be deployed to the brain and changes the genetic structure of a neurological cell. https://t.co/sqASWmrhjp

Jan 3, 2023 · 5 likes · 2 shares · 73 followers View post →
Thcisok
@Thcisok2 · X

Cas9 project crispr. Lekker blijven prikken. En gewoon doorscrollen. https://t.co/CwK1YcyJWY

Oct 20, 2021 · 3 likes · 3 shares · 1,574 followers View post →
Nathan VanDusen
@njvdn · X

@MayaTalukdar Yes, there's been some work with neurons. See this paper. This was what motivated us to try cardiomyocytes. https://t.co/co93Vj2Lwf

Mar 12, 2022 · 2 likes · 71 followers View post →
Özer
@OzerKhalid · X

RT @neuroweapons: This tool allows a virus to be deployed to the brain and changes the genetic structure of a neurological cell. https://…

Dec 10, 2025 · 2 shares · 39,404 followers View post →
Lifeboat Foundation
@LifeboatHQ · X

Making CRISPR-Cas9 work in the brain: Nishiyama et al. "Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain." Neuron (2017). doi:10․1016/j․neuron․2017․10. Video produced by https://t.co/QvamC2PQxN

Jun 10, 2023 · 30,750 followers View post →
Feiko ⚖️
@Psycho111177 · X

RT @Thcisok2: Cas9 project crispr. Lekker blijven prikken. En gewoon doorscrollen. https://t.co/CwK1YcyJWY

Jan 6, 2024 · 3 shares · 2,690 followers View post →

All mentions

Özer
@OzerKhalid · X

RT @neuroweapons: This tool allows a virus to be deployed to the brain and changes the genetic structure of a neurological cell. https://…

Dec 10, 2025 · 2 shares · 39,404 followers View post →
anja
@anja69203118 · X

RT @Thcisok2: Cas9 project crispr. Lekker blijven prikken. En gewoon doorscrollen. https://t.co/CwK1YcyJWY

Jan 6, 2024 · 3 shares · 576 followers View post →
MaxReid241120
@MaxReid241120 · X

RT @Thcisok2: Cas9 project crispr. Lekker blijven prikken. En gewoon doorscrollen. https://t.co/CwK1YcyJWY

Jan 6, 2024 · 3 shares · 910 followers View post →
Feiko ⚖️
@Psycho111177 · X

RT @Thcisok2: Cas9 project crispr. Lekker blijven prikken. En gewoon doorscrollen. https://t.co/CwK1YcyJWY

Jan 6, 2024 · 3 shares · 2,690 followers View post →
Lifeboat Foundation
@LifeboatHQ · X

Making CRISPR-Cas9 work in the brain: Nishiyama et al. "Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain." Neuron (2017). doi:10․1016/j․neuron․2017․10. Video produced by https://t.co/QvamC2PQxN

Jun 10, 2023 · 30,750 followers View post →
Neuroweapon Forensics
@neuroweapons · X

This tool allows a virus to be deployed to the brain and changes the genetic structure of a neurological cell. https://t.co/sqASWmrhjp

Jan 3, 2023 · 5 likes · 2 shares · 73 followers View post →
Nathan VanDusen
@njvdn · X

@MayaTalukdar Yes, there's been some work with neurons. See this paper. This was what motivated us to try cardiomyocytes. https://t.co/co93Vj2Lwf

Mar 12, 2022 · 2 likes · 71 followers View post →
Thcisok
@Thcisok2 · X

Cas9 project crispr. Lekker blijven prikken. En gewoon doorscrollen. https://t.co/CwK1YcyJWY

Oct 20, 2021 · 3 likes · 3 shares · 1,574 followers View post →
Thcisok
@Thcisok2 · X

Valt hier niets te zien, gewoon doorscrollen en lekker blijven prikken. https://t.co/CwK1YcyJWY

Oct 20, 2021 · 1,574 followers View post →
RRID Robot
@RobotRrid · X

RRIDs were included in the Neuron paper "Virus-Mediated Genome Editing via Homology-Directe…" (https://t.co/W1c9e5Pg9m). RRIDs like this improve reproducibility in scientific research. #methodsmatter #OpenScience

Aug 14, 2021 · 160 followers View post →
RRID Robot
@RobotRrid · X

The authors of "Virus-Mediated Genome Editing via Homology-Directe…" (https://t.co/W1c9e5Pg9m) included RRIDs in their paper! RRIDs like this improve reproducibility in scientific research. #accelerateopenscience #ReproducibleResearch

Jul 31, 2021 · 160 followers View post →
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