The overall tone balances scientific curiosity with skepticism, illustrated by comments like 'Cas9 project crispr. Lekker blijven prikken' and mentions of the technique's versatility, such as tagging proteins in the brain. The use of informal language and references to further research, including RRIDs and reproducibility, reflect both engagement with technical details and cautious optimism about its significance.
AgreementNeither agree nor disagree
The posts display mixed reactions, with some expressing curiosity and others being skeptical or dismissive of the technique's implications.
InterestModerate interest
There is a moderate level of interest, with users showing curiosity about the research and contemplating applications beyond neuroscience.
EngagementModerate engagement
Discussions include references to methods, such as the use of CRISPR-Cas9 and AAV, and mention practical considerations, indicating some depth of engagement.
ImpactModerate impact
The social discussions highlight potential impact in research and medicine, though some posts focus on the technological novelty rather than broader societal effects.
Abstract in brief
This study demonstrates that homology-directed repair (HDR) can be effectively used for precise genome editing not only in dividing cells but also in mature, non-dividing neurons in the mouse brain by combining CRISPR-Cas9 with adeno-associated virus (AAV) for delivery.
The researchers successfully tagged endogenous proteins in various brain cell types both in vitro (cell culture and brain slices) and in vivo (developing, adult, and diseased brains), highlighting the technique's versatility.
This advancement suggests that AAV and CRISPR-Cas9 mediated HDR could become valuable tools for detailed genetic modifications in neuroscience research, including studies on brain function and disease.]
RT @neuroweapons: This tool allows a virus to be deployed to the brain and changes the genetic structure of a neurological cell.
https://…
Dec 10, 2025 · 2 shares · 39,404 followersView post →
LF
Lifeboat Foundation
@LifeboatHQ · X
Making CRISPR-Cas9 work in the brain: Nishiyama et al. "Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain." Neuron (2017). doi:10․1016/j․neuron․2017․10.
Video produced by https://t.co/QvamC2PQxN
This video explains how homology-directed repair achieves accurate genome editing in both dividing and mature neurons in the mouse brain, using CRISPR-Cas9 combined with adeno-associated virus for delivery, enabling…
This study demonstrates that homology-directed repair can be used for precise genome editing in both dividing and mature neurons in the mouse brain by combining CRISPR-Cas9 with AAV delivery. It highlights the…
Jan 6, 2024 · 3 shares · 2,690 followersView post →
LF
Lifeboat Foundation
@LifeboatHQ · X
Making CRISPR-Cas9 work in the brain: Nishiyama et al. "Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain." Neuron (2017). doi:10․1016/j․neuron․2017․10.
Video produced by https://t.co/QvamC2PQxN
RRIDs were included in the Neuron paper "Virus-Mediated Genome Editing via Homology-Directe…" (https://t.co/W1c9e5Pg9m). RRIDs like this improve reproducibility in scientific research. #methodsmatter #OpenScience
The authors of "Virus-Mediated Genome Editing via Homology-Directe…" (https://t.co/W1c9e5Pg9m) included RRIDs in their paper! RRIDs like this improve reproducibility in scientific research. #accelerateopenscience #ReproducibleResearch
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