Science April 2018 PMID 29449508

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6.

Jonathan S Gootenberg, Omar O Abudayyeh, Max J Kellner, Julia Joung, James J Collins, Feng Zhang

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Mentions

Peak: 12 in Apr 2020

across 5 sources since Apr 2018

Extended reach

97,037

potential audience

Total likes

813

Video views

53,375

Mentions per month

Apr 2018 – Nov 2024

Where it's discussed

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AI synopsis

What people are saying

The ample use of phrases like 'excellent explanation' and mentions of CRISPR's role in diagnostics demonstrate enthusiasm and curiosity, while references to videos and potential mainstream use highlight high interest. The tone suggests a recognition of the publication's significance in advancing field diagnostics, with some posts engaging more deeply through technical details and implications.

AgreementModerate agreement

Most discussions acknowledge the importance and potential of CRISPR-based diagnostic platforms, reflecting general support.

InterestHigh interest

Participants are highly interested, as evidenced by their references to educational videos and discussions about mainstream adoption.

EngagementModerate engagement

The engagement is moderate; some posts share detailed insights or raise questions about clinical applications, but others are simple appreciations or references.

ImpactModerate impact

The discussions suggest that the article could influence future diagnostics, especially in rapid and portable testing, though the impact remains speculative at this stage.

Abstract in brief

SHERLOCKv2 is an advanced nucleic acid detection platform that combines isothermal preamplification with CRISPR enzymes like Cas13, Cas12a, and Csm6 for high-sensitivity, multiplexed, and portable detection of RNA and DNA.
It offers four key improvements: multi-channel multiplexing in a single reaction, ultra-sensitive quantification down to 2 attomolar, a 35-fold increase in signal sensitivity through enzyme combination, and a lateral flow readout for easy, rapid results.
The platform effectively detects viruses such as Dengue and Zika, as well as genetic mutations from patient samples, demonstrating its potential for clinical diagnostics, especially in field settings requiring quick, accurate, and multiplexed nucleic acid testing.]

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All mentions

CRISPR-based Multiplexed Diagnostics for Rapid Nucleic Acid Detection

A home test for Zika CRISPR may make it possible. This video explores how CRISPR-Cas systems are used in diagnostics for viruses like Zika and Dengue, highlighting the advanced SHERLOCKv2 platform that enables highly…

Science Magazine · Apr 27, 2018 53,262 views

Advanced CRISPR-Based Nucleic Acid Detection Platform for Diagnostics

SHERLOCKv2 combines isothermal amplification with CRISPR enzymes like Cas13, Cas12a, and Csm6 for highly sensitive, multiplexed, and portable RNA and DNA detection, enabling rapid diagnostics for viruses and genetic…

Alfred Patisenah · May 26, 2021 113 views

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Science explains: CRISPR diagnostics https://t.co/8uBSdegT3i via @YouTube

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