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Opt Lett April 2019 PMID 30933127

Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging.

Manish Kumar, Yevgenia Kozorovitskiy

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Mentions

35
Peak: 15 in Mar 2019
across 2 sources since Jan 2019

Extended reach

111,451
potential audience

Total likes

98

Video views

171

Mentions per month

Jan 2019 – Jan 2022

Where it's discussed

  • X / Twitter33 posts
  • YouTube2 videos

AI synopsis

What people are saying

The overall discussion highlights appreciation for the novel approach of tilt-invariance in large-scale volumetric imaging, as seen in phrases like 'important developments' and references to improved depth penetration and imaging quality. The tone varies from technical enthusiasm to practical considerations, such as camera choices, which reveals a blend of deep engagement and adaptive interest within the community. Words like 'hope,' 'support,' and mentions of 'published' work underscore the perceived importance and relevance of the publication.

AgreementModerate agreement

Most discussions express support or recognition of the publication's significance, with many emphasizing advancements and positive implications.

InterestHigh interest

Posts demonstrate high curiosity, referencing specific techniques like 1P light-sheet microscopy and depth penetration, indicating strong engagement with the topic.

EngagementModerate engagement

Several contributions share technical details, personal experiences, and commentary on camera quality and software, reflecting reasonable depth of discussion.

ImpactModerate impact

The discussions recognize the technological improvements and potential applications, suggesting users see moderate to high impact in these methods, though not universally or athoroughly.

Abstract in brief

  • This paper introduces a new method for large-scale 3D imaging in lightsheet microscopy, called multitile stitching, which allows for undistorted, high-resolution imaging of entire zebrafish larvae and thick mouse brain sections.
  • The researchers use remote galvo scanning to generate the lightsheet and develop a specialized processing pipeline that enables seamless tiling across different axes for comprehensive volumetric images.
  • Their improved one-photon, tilt-invariant SOPi microscope achieves cellular resolution at depths over 330 micrometers in scattering mouse brain tissue and captures detailed dendritic structures in more superficial layers.]
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Top posts

Most liked on X and Bluesky
Manish Kumar
@OptoManishK · X

Our next #SOPimicroscopy paper is published

Mar 22, 2019 · 51 likes · 13 shares · 1,187 followers View post →
Manish Kumar
@OptoManishK · X

@RetoPaul I believe we do enough science with cheap point grey cameras. Does this image quality satisfy your needs? Done with $1k point grey / FLIR camera [https://t.co/pWriaPm0To] https://t.co/FwFIr16FUB

Jun 18, 2019 · 11 likes · 1,187 followers View post →
Manish Kumar
@OptoManishK · X

Is death beautiful? This was an unintentional accidental recording (raw unprocessed data) done 2 years ago in zebrafish when our #SOPi microscope was first aligned. https://t.co/sZuOTaH0Ut https://t.co/pWriaPm0To https://t.co/Y8dkSSI7qb #lightsheet #SOPimicroscopy https://t.co/gGuls21lAN

Mar 14, 2020 · 10 likes · 4 shares · 1,187 followers View post →
Manish Kumar
@OptoManishK · X

Playing with #3Dscript plugin from @OICE_Imaging . One more dataset from the recent publication by me and @NUro_science. Imaging done with 1-photon #lightsheet using #SOPimicroscopy. Sample is Thy1GFP section fixed and uncleared. Further reading: https://t.co/pWriaPm0To https://t.co/wv6OJkgxwc

Mar 27, 2019 · 7 likes · 3 shares · 1,187 followers View post →
Genia Kozorovitskiy
@NUro_science · X

This builds on important prior developments by @OptoManishK for OPM style scopes, generating tilt invariance (https://t.co/HVDBPXdMUq), scaling up lower NA applications (https://t.co/MAyKfMsCjc), and implementing simple 1P and 2P light-sheet variants (https://t.co/yLvxHg3YbS).

May 3, 2021 · 4 likes · 2,353 followers View post →
Manish Kumar
@OptoManishK · X

@schoppik @pfapostolides @mjhigley @AndrewHires @mnitabach @analog_ashley I love FLIR cameras for my lightsheet stuff. As @schoppik mentioned, you need to consider the software for the camera as well before settling for one. Image quality wise here is what Grasshopper from FLIR did for us https://t.co/pWriaPm0To https://t.co/uBxStnkzsv

Dec 18, 2019 · 4 likes · 1,187 followers View post →

All mentions

Manish Kumar
@OptoManishK · X

@Nano_Juli @RetoPaul @AndrewGYork @kD3AN @loicaroyer @Bin_YANG_Optics @DougPShepherd @kschink @florianstroehl @Maurice_Y_Lee @CellJeffe @fspasqualini @IlariaTesta4 @Bodeeen1 @cyclocrossguy You may see some 2P results with side by side comparison in our 2018 paper https://t.co/sZuOTaH0Ut How much resolution do you need at 60 µm? We are happy with 1P light-sheet for many applications: > 300 µm deep cellular resolution (fixed mouse brain) https://t.co/pWriaPm0To

Jun 9, 2021 · 3 likes · 1,187 followers View post →
Genia Kozorovitskiy
@NUro_science · X

This builds on important prior developments by @OptoManishK for OPM style scopes, generating tilt invariance (https://t.co/HVDBPXdMUq), scaling up lower NA applications (https://t.co/MAyKfMsCjc), and implementing simple 1P and 2P light-sheet variants (https://t.co/yLvxHg3YbS).

May 3, 2021 · 4 likes · 2,353 followers View post →
Manish Kumar
@OptoManishK · X

@JacquesPaysan Depends on configuration, and resolution requirements. https://t.co/sZuOTaH0Ut (1P + 2P at > 0.7 mm FOV) https://t.co/pWriaPm0To (deeper than 300 μm for cells in 2 stitches)

Feb 28, 2021 · 1,187 followers View post →
Manish Kumar
@OptoManishK · X

@wiebkejahr @kD3AN @RetoPaul @lorenzo_corinne @FahrbachFlo This is true even in our personal experience. With Gaussian light-sheet (DSLM implementation) we gained on depth penetration and contrast of imaging in scattering samples. (left- cylindrical lens based light-sheet, right- DSLM Gaussian light-sheet) https://t.co/pWriaPm0To https://t.co/5NUEjDnxBC

Jun 14, 2020 · 1 like · 1,187 followers View post →
Manish Kumar
@OptoManishK · X

@schoppik @Maurice_Y_Lee @ScopeKurt This blog had given me hope to try Grasshopper camera from point grey/FLIR which then made to our publication https://t.co/pWriaPm0To

May 9, 2020 · 2 likes · 1 share · 1,187 followers View post →
Özge Çark
@ozuozge · X

RT @OptoManishK: Is death beautiful? This was an unintentional accidental recording (raw unprocessed data) done 2 years ago in zebrafish…

Mar 15, 2020 · 4 shares · 779 followers View post →
Zebrafish Rock!
@ZebrafishRock · X

RT @OptoManishK: Is death beautiful? This was an unintentional accidental recording (raw unprocessed data) done 2 years ago in zebrafish…

Mar 15, 2020 · 4 shares · 11,772 followers View post →
⚡️GREG_CORDER⚡️
@FlyBottleEscape · X

RT @OptoManishK: Is death beautiful? This was an unintentional accidental recording (raw unprocessed data) done 2 years ago in zebrafish…

Mar 14, 2020 · 4 shares · 3,654 followers View post →
Panos Symvoulidis
@symvou · X

RT @OptoManishK: Is death beautiful? This was an unintentional accidental recording (raw unprocessed data) done 2 years ago in zebrafish…

Mar 14, 2020 · 4 shares · 1,256 followers View post →
Manish Kumar
@OptoManishK · X

Is death beautiful? This was an unintentional accidental recording (raw unprocessed data) done 2 years ago in zebrafish when our #SOPi microscope was first aligned. https://t.co/sZuOTaH0Ut https://t.co/pWriaPm0To https://t.co/Y8dkSSI7qb #lightsheet #SOPimicroscopy https://t.co/gGuls21lAN

Mar 14, 2020 · 10 likes · 4 shares · 1,187 followers View post →
Science Alerts
@Science_Alerts · X

RT @EurekaMag: Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging https://t.co/5wPwXymZBB

Jan 26, 2020 · 2 shares · 1,234 followers View post →
POPLINE
@POPLINE_org · X

RT @EurekaMag: Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging https://t.co/5wPwXymZBB

Jan 26, 2020 · 2 shares · 5,238 followers View post →
EurekaMag
@EurekaMag · X

Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging https://t.co/5wPwXymZBB

Jan 26, 2020 · 2 shares · 6,543 followers View post →
Manish Kumar
@OptoManishK · X

@schoppik @pfapostolides @mjhigley @AndrewHires @mnitabach @analog_ashley I love FLIR cameras for my lightsheet stuff. As @schoppik mentioned, you need to consider the software for the camera as well before settling for one. Image quality wise here is what Grasshopper from FLIR did for us https://t.co/pWriaPm0To https://t.co/uBxStnkzsv

Dec 18, 2019 · 4 likes · 1,187 followers View post →
Beckman Foundation
@BeckmanFnd · X

READ: 2015 #BYI Yevgenia Kozorovitskiy @NUro_science was published in the journal Optics Letters: "Tilt-invariant Scanned Oblique Plane Illumination Microscopy for Large-scale Volumetric Imaging." https://t.co/cGMaRVo9Fq

Sep 20, 2019 · 3 likes · 2,331 followers View post →
N Wanaverbecq
@N_Wanaverbecq · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Jun 29, 2019 · 13 shares · 170 followers View post →
Manish Kumar
@OptoManishK · X

@RetoPaul I believe we do enough science with cheap point grey cameras. Does this image quality satisfy your needs? Done with $1k point grey / FLIR camera [https://t.co/pWriaPm0To] https://t.co/FwFIr16FUB

Jun 18, 2019 · 11 likes · 1,187 followers View post →
Beckman Foundation
@BeckmanFnd · X

READ: ‘15 #BYI Yevgenia Kozorovitskiy was published in the journal Optics Letters: "Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging." https://t.co/RWyFZxrFT7 https://t.co/A5veR06fNt

Apr 17, 2019 · 2 likes · 2,331 followers View post →
Volodymyr Nechyporuk-Zloy
@vnzloy · X

Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging Manish Kumar and Yevgenia Kozorovitskiy https://t.co/x2NQuBYv5f https://t.co/WBxyEyh8Q5

Apr 8, 2019 · 1,287 followers View post →
BioloGeek
@Nico_BioloGeek · X

RT @OptoManishK: Playing with #3Dscript plugin from @OICE_Imaging . One more dataset from the recent publication by me and @NUro_science. I…

Mar 28, 2019 · 3 shares · 1,732 followers View post →
Muralidhar B
@bmurali1987 · X

RT @OptoManishK: Playing with #3Dscript plugin from @OICE_Imaging . One more dataset from the recent publication by me and @NUro_science. I…

Mar 27, 2019 · 3 shares · 321 followers View post →
Manish Kumar
@OptoManishK · X

Playing with #3Dscript plugin from @OICE_Imaging . One more dataset from the recent publication by me and @NUro_science. Imaging done with 1-photon #lightsheet using #SOPimicroscopy. Sample is Thy1GFP section fixed and uncleared. Further reading: https://t.co/pWriaPm0To https://t.co/wv6OJkgxwc

Mar 27, 2019 · 7 likes · 3 shares · 1,187 followers View post →
Jun-Liszt Li
@poppingjun · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Mar 24, 2019 · 13 shares · 1,030 followers View post →
Mark Kittisopikul
@markkitti · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Mar 23, 2019 · 13 shares · 1,258 followers View post →
BioloGeek
@Nico_BioloGeek · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Mar 23, 2019 · 13 shares · 1,732 followers View post →
Erlich Lab
@erlichlab · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Mar 22, 2019 · 13 shares · 759 followers View post →
gerald pao
@PLURplus · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Mar 22, 2019 · 13 shares · 990 followers View post →
Genia Kozorovitskiy
@NUro_science · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Mar 22, 2019 · 13 shares · 2,353 followers View post →
Wilson Adams
@wradamsphd · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Mar 22, 2019 · 13 shares · 2,057 followers View post →
Rita Strack
@rita_strack · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Mar 22, 2019 · 13 shares · 21,532 followers View post →
OpenSPIM
@openspim · X

RT @OptoManishK: Our next #SOPimicroscopy paper is published

Mar 22, 2019 · 13 shares · 2,447 followers View post →
Manish Kumar
@OptoManishK · X

Our next #SOPimicroscopy paper is published

Mar 22, 2019 · 51 likes · 13 shares · 1,187 followers View post →
BlackPhysicists
@BlackPhysicists · X

Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging. (arXiv:1901.00498v1 [physics.optics]) https://t.co/EH1cjm7f4g #biophysics

Jan 4, 2019 · 25,080 followers View post →