Neuron August 2019 PMID 31272828

Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering.

Yudong Gao, Erin Hisey, Tyler W A Bradshaw, Eda Erata, Walter E Brown, Jamie L Courtland, Akiyoshi Uezu, Yu Xiang, Yarui Diao, Scott H Soderling

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Mentions

Peak: 5 in Jul 2019

across 2 sources since Jul 2019

Extended reach

11,905

potential audience

Total likes

Video views

1,491

Mentions per month

Jul 2019 – Mar 2022

Where it's discussed

X / Twitter6 posts
YouTube1 videos

AI synopsis

What people are saying

Discussions strongly support the potential of HiUGE, with phrases like 'hope it will be huge' and 'high-throughput gene editing,' emphasizing excitement and optimism. The tone is enthusiastic, and examples include mentions of rapid modifications, neural applications, and the method's versatility, reflecting high engagement and belief in its transformative impact.

AgreementModerate agreement

Most discussions express support or excitement for the new genome editing technique, highlighting its potential benefits.

InterestHigh interest

The discussions demonstrate high curiosity, particularly focusing on the method's speed, versatility, and applications in neural research.

EngagementHigh engagement

Participants actively share their thoughts, referencing the publication's content, uses, and implications, showing deep involvement.

ImpactHigh impact

The enthusiasm and detailed references suggest the method could be a significant advancement in molecular biology and neuroscience.

Abstract in brief

The study highlights the importance of analyzing protein localization and function in various cell types, especially in the brain.
Current methods are often inefficient and resource-consuming, leading to the development of a new CRISPR-Cas9-based technique called HiUGE for easier gene manipulation.
HiUGE allows for rapid and flexible modifications to proteins using AAV vectors, facilitating a range of applications like protein labeling, neural modifications, and structure-function analyses in a high-throughput manner.

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All mentions

Efficient Protein Modification with Homology-independent Genome Engineering

Gao et al. introduce HiUGE, a CRISPR-Cas9-based method for rapid, flexible protein modifications using AAV vectors, enabling high-throughput analysis of protein function and localization, particularly in neural studies.

AJE Video Bytes · Jul 1, 2019 1,491 views

Don Indispaunible

@Indispaunible · X

RT @dukeresearch: This is HiUGE, and we hope it will be huge too. High-throughput gene editing with "plug and play" #CRISPR sequences. (Neu…

Mar 1, 2022 · 2 shares · 149 followers View post →

CRISP_SCIENCE

@ScienceCrisp · X

HiUGE "Plug-and-Play Protein Modification Using HomologyIndependent Universal Genome Engineering" Gao Y [..] Soderling SH. Neuron 2019-07-01. https://t.co/mhFc6IffoU https://t.co/fyjjUlWLPE

Apr 11, 2020 · 1,918 followers View post →

Duke OTC

@DukeOTC · X

RT @dukeresearch: This is HiUGE, and we hope it will be huge too. High-throughput gene editing with "plug and play" #CRISPR sequences. (Neu…

Jul 2, 2019 · 2 shares · 674 followers View post →

Duke Research & Innovation

@dukeresearch · X

This is HiUGE, and we hope it will be huge too. High-throughput gene editing with "plug and play" #CRISPR sequences. (Neuron, July 1, 2019.) VIDEO https://t.co/mxMv936Eyw https://t.co/Suv1QULt9b

Jul 2, 2019 · 4 likes · 2 shares · 4,752 followers View post →

Alex Chamessian

@achamess · X

Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering: Neuron https://t.co/Ft5aiDDJ8s

Jul 1, 2019 · 3 likes · 2,742 followers View post →

MyNeuroNews.com

@MyNeuroNewscom · X

(Plug-and-Play Protein Modification Using Homology-Independent Universal Genome ...) has been published on MyN ... - https://t.co/nHf9c4CdRQ

Jul 1, 2019 · 167 followers View post →