October 2021 PMID 33164641

Measurement of autophagic flux in humans: an optimized method for blood samples.

Julien Bensalem, Kathryn J Hattersley, Leanne K Hein, Xiao Tong Teong, Julian M Carosi, Sofia Hassiotis, Randall H Grose, Célia Fourrier, Leonie K Heilbronn, Timothy J Sargeant

Mentions

38
Peak: 7 in Nov 2020
across 3 sources since Nov 2020

Extended reach

61,562
potential audience

Total likes

124

Video views

2,744

Mentions per month

Nov 2020 – May 2024

Where it's discussed

  • X / Twitter27 posts
  • News9 articles
  • YouTube2 videos

AI synopsis

What people are saying

Several posts celebrate the publication of the new method, with phrases like 'proud of our lab' and 'new assay for measuring autophagy in humans,' highlighting a sense of achievement and curiosity. The tone is positive and collaborative, emphasizing the method’s potential to advance understanding of human health, which underscores the perceived significance of the research's impact.

AgreementModerate agreement

Most discussions express support for the publication, highlighting pride in the new method and its potential applications, indicating general alignment with the significance of the research.

InterestHigh interest

The discussions show high interest, with several posts mentioning the clinical relevance and excitement about measuring autophagy in humans, suggesting engagement with practical implications.

EngagementModerate engagement

While some posts celebrate the publication and share enthusiasm, others reference the technical aspects or collaboration efforts, indicating moderate but focused engagement.

ImpactModerate impact

The posts imply that the method could have meaningful impact on health monitoring and disease research, as users mention its potential importance in understanding factors like aging and neurodegenerative diseases.

Abstract in brief

  • This study introduces a new method to measure autophagic flux directly from human blood samples by using a lysosomal inhibitor, chloroquine, to assess the activity of autophagy-related proteins like LC3BII, preserving individual genetic and nutritional factors.
  • The method was shown to be reproducible and effective in detecting changes in autophagy levels, especially after manipulating nutrition signaling with substances like leucine and insulin, highlighting its potential to monitor autophagic activity in humans.
  • Ultimately, this technique could help identify factors influencing autophagy in humans, serve as a biomarker for age-related diseases, and improve understanding of how autophagic processes impact human health and disease risk.]

Top posts

Most liked on X and Bluesky
Assoc. Prof. Tim Sargeant
@TimSargeant1 · X

Really proud of @JulienBensalem and the lab for devising a method for measuring #autophagy in human blood, with wonderful collaborator A/Prof Leonie Heilbronn! @sahmriAU Measurement of autophagic flux in humans: an optimized method for blood samples https://t.co/S6R6rvJKUa

Nov 9, 2020 · 24 likes · 3 shares · 1,115 followers View post →
Julian Carosi
@JulianMCarosi · X

Our lab set out to answer a simple question - how can we measure autophagy in people? And here it is. Honoured to be able to contribute to this work. Congratulations to @JulienBensalem @Celia_Fourrier and @TimSargeant1 etal,. https://t.co/fHajUs77OV

Nov 9, 2020 · 15 likes · 1 share · 412 followers View post →
Dr Célia Fourrier
@Celia_Fourrier · X

Accidentally deleted tweet

Jun 1, 2021 · 10 likes · 5 shares · 121 followers View post →
Julien Bensalem
@JulienBensalem · X

Last year we published a new method to measure #autophagy function in human blood: https://t.co/2f4ps5mpkp This week, Dr @TimSargeant1 presented one of its clinical applications to @10NewsFirstAdl. @sahmriAU https://t.co/LQm4SwYMWZ

Jul 7, 2021 · 7 likes · 4 shares · 132 followers View post →
SAHMRI
@sahmriAU · X

#WorldAlzheimersMonth Using a new SAHMRI-developed

Sep 23, 2021 · 6 likes · 4 shares · 6,510 followers View post →
Julian Carosi
@JulianMCarosi · X

.@PeterAttiaMD I think we have something you’d be interested in... an assay to measure autophagy in people. @TimSargeant1 @JulienBensalem https://t.co/sIVQqmLi7z

Nov 9, 2020 · 3 likes · 1 share · 412 followers View post →

All mentions

Marina García Macia
@Juanaloca2002 · X

RT @TimSargeant1: We took our protocol for measuring autophagic flux in whole blood culture that was published previously in Autophagy (htt…

Aug 25, 2023 · 1 share · 2,817 followers View post →
Assoc. Prof. Tim Sargeant
@TimSargeant1 · X

We took our protocol for measuring autophagic flux in whole blood culture that was published previously in Autophagy (https://t.co/uT7kWPwVOP) and we adapted this technique for use with an ELISA from @CellSignal. This enabled #autophagy measurement in larger human cohorts.

Jul 27, 2023 · 2 likes · 1 share · 1,115 followers View post →
Lee Stevenson
@bashuser · X

Measurement of autophagic flux in humans: an optimized method for blood samples https://t.co/8ygsZdXNc3

Nov 13, 2022 · 1,683 followers View post →
Jin Li,Ph.D.
@AnnysTibbers · X

RT @JulianMCarosi: .@PeterAttiaMD I think we have something you’d be interested in... an assay to measure autophagy in people. @TimSargeant…

Nov 5, 2021 · 1 share · 12 followers View post →
BrightFocus Foundation
@_BrightFocus · X

RT @sahmriAU: #WorldAlzheimersMonth Using a new SAHMRI-developed

Sep 23, 2021 · 4 shares · 17,151 followers View post →
sea-geo.bsky.social
@SeanGeoghegan · X

RT @sahmriAU: #WorldAlzheimersMonth Using a new SAHMRI-developed

Sep 23, 2021 · 4 shares · 2,756 followers View post →
SAHMRI
@sahmriAU · X

#WorldAlzheimersMonth Using a new SAHMRI-developed

Sep 23, 2021 · 6 likes · 4 shares · 6,510 followers View post →
Sophie Layé
@NutriNeuro · X

RT @JulienBensalem: Last year we published a new method to measure #autophagy function in human blood: https://t.co/2f4ps5mpkp This week, D…

Jul 31, 2021 · 4 shares · 1,046 followers View post →
Food4BrainHealth
@food4_brain · X

RT @JulienBensalem: Last year we published a new method to measure #autophagy function in human blood: https://t.co/2f4ps5mpkp This week, D…

Jul 31, 2021 · 4 shares · 215 followers View post →
Dr Célia Fourrier
@Celia_Fourrier · X

RT @JulienBensalem: Last year we published a new method to measure #autophagy function in human blood: https://t.co/2f4ps5mpkp This week, D…

Jul 7, 2021 · 4 shares · 121 followers View post →
Assoc. Prof. Tim Sargeant
@TimSargeant1 · X

RT @JulienBensalem: Last year we published a new method to measure #autophagy function in human blood: https://t.co/2f4ps5mpkp This week, D…

Jul 7, 2021 · 4 shares · 1,115 followers View post →
Julien Bensalem
@JulienBensalem · X

Last year we published a new method to measure #autophagy function in human blood: https://t.co/2f4ps5mpkp This week, Dr @TimSargeant1 presented one of its clinical applications to @10NewsFirstAdl. @sahmriAU https://t.co/LQm4SwYMWZ

Jul 7, 2021 · 7 likes · 4 shares · 132 followers View post →
Marina García Macia
@Juanaloca2002 · X

RT @Celia_Fourrier: Accidentally deleted tweet

Jun 2, 2021 · 5 shares · 2,817 followers View post →
Women in Autophagy
@WAutophagy · X

RT @Celia_Fourrier: Accidentally deleted tweet

Jun 2, 2021 · 5 shares · 2,648 followers View post →
Rapamycin News
@RapaNews · X

RT @Celia_Fourrier: Accidentally deleted tweet

Jun 1, 2021 · 5 shares · 5,345 followers View post →
Julien Bensalem
@JulienBensalem · X

RT @Celia_Fourrier: Accidentally deleted tweet

Jun 1, 2021 · 5 shares · 132 followers View post →
Assoc. Prof. Tim Sargeant
@TimSargeant1 · X

RT @Celia_Fourrier: Accidentally deleted tweet

Jun 1, 2021 · 5 shares · 1,115 followers View post →
Dr Célia Fourrier
@Celia_Fourrier · X

Accidentally deleted tweet

Jun 1, 2021 · 10 likes · 5 shares · 121 followers View post →
Sophie Layé
@NutriNeuro · X

RT @TimSargeant1: Really proud of @JulienBensalem and the lab for devising a method for measuring #autophagy in human blood, with wonderful…

Apr 29, 2021 · 3 shares · 1,046 followers View post →
Mahmoud Abdellatif
@Mah_abdellatif · X

Measurement of autophagic flux in humans: an optimized method for blood samples. https://t.co/AwKsKDSu10

Mar 13, 2021 · 1 like · 624 followers View post →
Rapamycin News
@RapaNews · X

RT @TimSargeant1: Really proud of @JulienBensalem and the lab for devising a method for measuring #autophagy in human blood, with wonderful…

Nov 30, 2020 · 3 shares · 5,345 followers View post →
T&F Bioscience
@tandfbiosci · X

RT @JulienBensalem: Very proud to see our new paper published in Autophagy where we provide a new method for measuring #autophagy in human…

Nov 10, 2020 · 1 share · 2,605 followers View post →
John Sargeant
@Artfromnz · X

RT @TimSargeant1: Really proud of @JulienBensalem and the lab for devising a method for measuring #autophagy in human blood, with wonderful…

Nov 9, 2020 · 3 shares · 59 followers View post →
Julian Carosi
@JulianMCarosi · X

.@PeterAttiaMD I think we have something you’d be interested in... an assay to measure autophagy in people. @TimSargeant1 @JulienBensalem https://t.co/sIVQqmLi7z

Nov 9, 2020 · 3 likes · 1 share · 412 followers View post →
Kat Stevens
@KatStevens_au · X

RT @JulianMCarosi: Our lab set out to answer a simple question - how can we measure autophagy in people? And here it is. Honoured to be abl…

Nov 9, 2020 · 1 share · 188 followers View post →
Julian Carosi
@JulianMCarosi · X

Our lab set out to answer a simple question - how can we measure autophagy in people? And here it is. Honoured to be able to contribute to this work. Congratulations to @JulienBensalem @Celia_Fourrier and @TimSargeant1 etal,. https://t.co/fHajUs77OV

Nov 9, 2020 · 15 likes · 1 share · 412 followers View post →
Assoc. Prof. Tim Sargeant
@TimSargeant1 · X

Really proud of @JulienBensalem and the lab for devising a method for measuring #autophagy in human blood, with wonderful collaborator A/Prof Leonie Heilbronn! @sahmriAU Measurement of autophagic flux in humans: an optimized method for blood samples https://t.co/S6R6rvJKUa

Nov 9, 2020 · 24 likes · 3 shares · 1,115 followers View post →