Water Res X May 2021 PMID 33490943

Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications - A systematic review.

Mats Leifels, Dan Cheng, Emanuele Sozzi, David C Shoults, Stefan Wuertz, Skorn Mongkolsuk, Kwanrawee Sirikanchana

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Mentions

12
Peak: 5 in May 2020
across 2 sources since May 2020

Extended reach

35,953
potential audience

Total likes

4

Video views

27

Mentions per month

May 2020 – Jan 2024

Where it's discussed

  • X / Twitter9 posts
  • YouTube3 videos

AI synopsis

What people are saying

The social discussions reflect a generally positive and interested attitude toward the article, with examples like researchers sharing preprints and calling for reviews, and hashtags emphasizing its importance for water quality and viral detection. The tone is informative and collaborative, showing recognition of its potential impact on environmental monitoring and outbreak response.

AgreementModerate agreement

Most discussions acknowledge the relevance of the systematic review, especially in the context of viral detection and public health.

InterestModerate interest

Users show moderate interest by sharing the publication and related preprints, indicating engagement but not deep critique or exploration.

EngagementModerate engagement

Comments show active participation through sharing, retweeting, and requesting reviews, reflecting ongoing consideration of the topic.

ImpactModerate impact

The discussions suggest the publication influences ongoing research and public health strategies by highlighting innovative molecular techniques.

Abstract in brief

  • The review summarizes how capsid integrity quantitative PCR (qPCR) combined with dye pretreatment is used to detect infectious viruses in food safety and environmental settings, noting that protocol variations can affect accuracy.
  • It highlights that dyes like ethidium monoazide are effective in identifying viruses with intact capsids, but less so for viruses inactivated by other means, suggesting the need for virus-specific protocol optimization.
  • The authors propose a general framework for assay conditions, including dye concentrations, incubation times, and light activation methods, to improve virus detection during water monitoring and viral outbreak responses such as COVID-19 and dengue fever.]

Top posts

Most liked on X and Bluesky
medRxiv
@medrxivpreprint · X

Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications; a systematic review https://t.co/qb7275MPz5 #medRxiv

May 12, 2020 · 2 likes · 17,460 followers View post →
Outbreak Science
@outbreaksci · X

Contribute a rapid review or request one for "Capsid integrity quantitative PCR to determine virus infectivity in environmental and ..." (https://t.co/m63U0O7p7r). "Add" or "Request" w/DOI (10.1101/2020.05.08.20095364) at https://t.co/RnurTL3DyM @outbreaksci @PREreview_

May 13, 2020 · 1 like · 1 share · 1,377 followers View post →
K.Koiwai
@KKo1wa1 · X

とりあえずこの方法がPCRとか噛ますなら現実的かも?! Here is the link to "Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications – A systematic review": https://t.co/c3iYWdU9f1

Jan 17, 2024 · 1 like · 1,098 followers View post →
SystematicReviewBot
@EvidenceRobot · X

RT @COVID19arXiv:

May 12, 2020 · 1 share · 6,710 followers View post →
SystematicReviewBot
@EvidenceRobot · X

RT @outbreaksci: New #dengue preprint: Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applicati…

May 13, 2020 · 1 share · 6,710 followers View post →
Outbreak Science
@outbreaksci · X

New #dengue preprint: Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications; a systematic review https://t.co/m63U0O7p7r

May 13, 2020 · 1 share · 1,377 followers View post →

All mentions

K.Koiwai
@KKo1wa1 · X

とりあえずこの方法がPCRとか噛ますなら現実的かも?! Here is the link to "Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications – A systematic review": https://t.co/c3iYWdU9f1

Jan 17, 2024 · 1 like · 1,098 followers View post →
Imambiental
@Imambiental · X

RT @Imambiental: Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications – A systematic rev…

Nov 13, 2023 · 2 shares · 343 followers View post →
Henry A. Maury
@hamaurytw · X

RT @Imambiental: Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications – A systematic rev…

Nov 13, 2023 · 2 shares · 489 followers View post →
Imambiental
@Imambiental · X

Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications – A systematic review. #6azodye #EMA #PMA #Microbialcontamination #virusinfectivity #Waterquality https://t.co/TzuFRhWcWa

Nov 13, 2023 · 2 shares · 343 followers View post →
SystematicReviewBot
@EvidenceRobot · X

RT @outbreaksci: New #dengue preprint: Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applicati…

May 13, 2020 · 1 share · 6,710 followers View post →
Outbreak Science
@outbreaksci · X

Contribute a rapid review or request one for "Capsid integrity quantitative PCR to determine virus infectivity in environmental and ..." (https://t.co/m63U0O7p7r). "Add" or "Request" w/DOI (10.1101/2020.05.08.20095364) at https://t.co/RnurTL3DyM @outbreaksci @PREreview_

May 13, 2020 · 1 like · 1 share · 1,377 followers View post →
Outbreak Science
@outbreaksci · X

New #dengue preprint: Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications; a systematic review https://t.co/m63U0O7p7r

May 13, 2020 · 1 share · 1,377 followers View post →
SystematicReviewBot
@EvidenceRobot · X

RT @COVID19arXiv:

May 12, 2020 · 1 share · 6,710 followers View post →
medRxiv
@medrxivpreprint · X

Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications; a systematic review https://t.co/qb7275MPz5 #medRxiv

May 12, 2020 · 2 likes · 17,460 followers View post →