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Curr Protoc February 2021 PMID 33534198

Generation of Neurosphere-Derived Organoid-Like-Aggregates (NEDAS) from Neural Stem Cells.

Fumihiro Watanabe, Austin Schoeffler, Summer R Fair, Mark E Hester, Josh Fedorko, Jaime Imitola

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Mentions

5
Peak: 3 in Feb 2021
across 2 sources since Feb 2021

Extended reach

5,373
potential audience

Total likes

19

Video views

2,471

Mentions per month

Feb 2021 – Apr 2022

Where it's discussed

  • X / Twitter3 posts
  • YouTube2 videos

AI synopsis

What people are saying

Discussions highlight the innovative aspects of NEDAS, such as its potential to improve neural stem cell cultures, with phrases like 'alternative' and 'complementary' demonstrating openness. Words like 'proud' and 'congratulations' convey a tone of respect and recognition, emphasizing the significance of the research while indicating a cautious optimism about its future impact.

AgreementModerate agreement

Most comments acknowledge the new NEDAS method as a valuable development, with some noting it as an alternative or complement to existing techniques, indicating general support.

InterestHigh interest

Discussions show high enthusiasm for the research, especially with mentions of interest in neural progenitor behavior and potential applications.

EngagementModerate engagement

The comments include expressions of pride and congratulations, reflecting active recognition, but without in-depth technical debates.

ImpactModerate impact

The posts suggest the research could influence future culture methods and neural studies, though they do not state it will revolutionize the field.

Abstract in brief

  • Neurosphere cultures have been used to study neural stem cells (NSCs) for over 20 years but come with limitations, like fusion behavior which affects long-term results.
  • The Neurosphere-derived organoid-like aggregates (NEDAS) method improves on traditional cultures by allowing NSCs to fuse in a high-density environment, creating 3D organoid-like structures without using Matrigel, and maintaining them for long periods.
  • NEDAS organoids exhibit proliferation and self-organization into neural structures, presenting diverse cell types, and offer a new protocol for studying NSC dynamics and morphogenesis, potentially complementing existing cerebral organoid methods.
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Top posts

Most liked on X and Bluesky
Austin
@AustinSchoeff · X

Congratulations @DrJim4MS and team for creating a new method for culturing neural stem cells in 3-Dimensions! Proud to have contributed to this amazing research -https://t.co/qFwsqTNXze

Feb 3, 2021 · 6 likes · 432 followers View post →
Jaime Imitola MD., FAAN
@DrJim4MS · X

We present our NEDAS method, which can be an alternative to the traditional neurospheres method and complementary to other 3D culture methods (not a replacement). We are interested in how non-iPSCs neural progenitors behave in 3D. https://t.co/lVPwApfBMM @UConnResearch #organoids https://t.co/XANu3tIerK

Feb 3, 2021 · 2 likes · 2,014 followers View post →

All mentions

Paperbirds_Neurology
@PaperbirdsN · X

New article: Generation of Neurosphere-Derived Organoid-Like-Aggregates (NEDAS) from Neural Stem Cells. https://t.co/KgXr3kDiqM #MS #multiplesclerosis #neurology https://t.co/88zXEj1c4h

Feb 4, 2021 · 457 followers View post →
Jaime Imitola MD., FAAN
@DrJim4MS · X

We present our NEDAS method, which can be an alternative to the traditional neurospheres method and complementary to other 3D culture methods (not a replacement). We are interested in how non-iPSCs neural progenitors behave in 3D. https://t.co/lVPwApfBMM @UConnResearch #organoids https://t.co/XANu3tIerK

Feb 3, 2021 · 2 likes · 2,014 followers View post →
Austin
@AustinSchoeff · X

Congratulations @DrJim4MS and team for creating a new method for culturing neural stem cells in 3-Dimensions! Proud to have contributed to this amazing research -https://t.co/qFwsqTNXze

Feb 3, 2021 · 6 likes · 432 followers View post →