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PLoS Genet May 2021 PMID 33999957

Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster.

Leslie M Stevens, Goheun Kim, Theodora Koromila, John W Steele, James McGehee, Angelike Stathopoulos, David S Stein

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Mentions

29
Peak: 15 in May 2021
across 2 sources since May 2021

Extended reach

239,256
potential audience

Total likes

86

Video views

516

Mentions per month

May 2021 – May 2024

Where it's discussed

  • X / Twitter22 posts
  • YouTube7 videos

AI synopsis

What people are saying

The discussions reveal strong support and excitement about the study's advancements, such as the development of the photoNdegron system, with users emphasizing its potential to enhance research modalities. Words like 'excited,' 'collaborate,' and 'game changer' illustrate enthusiasm, while mentions of specific applications showcase deep engagement and recognition of the article's significance.

AgreementModerate agreement

Most discussions express enthusiasm and support for the publication, highlighting its novelty and potential applications.

InterestHigh interest

Posts show high interest, with many users excited about the new light-controlled protein degradation techniques and their implications.

EngagementHigh engagement

Several comments reference technical details, collaborations, and future use cases, reflecting deep engagement.

ImpactModerate impact

While the enthusiasm is clear, the perceived immediate practical impact is positive but not groundbreaking for all users.

Abstract in brief

  • The text discusses the development of the photoNdegron, a peptide tag that allows for light-controlled degradation of proteins in living organisms like yeast and fruit flies, enabling precise research on protein function.
  • It explains how the photoNdegron works by changing shape under blue light to expose signals for degradation, and compares its effectiveness to other light-activated degrons, such as the BLID domain and the PSD, in targeting specific proteins like Cactus in Drosophila embryos.
  • The findings highlight that choosing the right light-dependent degron is crucial, as some are more effective than others in manipulating protein activity, and that both N-terminal and C-terminal fusions can be used successfully for in vivo protein regulation.]
Read the full abstract

Top posts

Most liked on X and Bluesky
Stathopoulos Lab
@stathlab · X

We've seen the light, and with great spatial and temporal resolution, too!

May 17, 2021 · 22 likes · 4 shares · 171 followers View post →
Theodora Koromila
@t_koromila · X

Excited that our @PLOSGenetics paper is out! A new photo-N-degron that can be used in Optogenetic in vivo studies is now available https://t.co/tGMu95pDFx! Fun to collaborate with the Stein lab! Also, thank you @Angela_Stathop, David Stein and James McGehee

May 17, 2021 · 14 likes · 5 shares · 295 followers View post →
PLOS Genetics
@PLOSGenetics · X

Stevens et al identify and describe a new #peptide degradation signal, the photo-N-degron When fused to the N-terminus of a protein, it can induce N-end rule-mediated degradation in response to blue-light illumination

Aug 1, 2021 · 12 likes · 4 shares · 34,736 followers View post →
Molecular Biosciences
@ut_mbs · X

Congratulations, @ut_mbs @TexasScience Dr. Leslie Stevens, Prof. David Stein & the Team for publication in @PLOSGenetics "Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster" https://t.co/yDW6vfIQVE https://t.co/l7Npt0gLz2

Nov 8, 2021 · 11 likes · 2 shares · 689 followers View post →
@luschnig.bsky.social
@StefanLuschnig · X

#PLOSGenetics: Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae ... https://t.co/htzq7kxxFM

May 18, 2021 · 2 likes · 2 shares · 1,158 followers View post →
Dougan Lab
@DouganLab · X

#PLOSGenetics: Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae ... https://t.co/sR2ANgzKh4

May 20, 2021 · 2 likes · 1 share · 933 followers View post →

All mentions

Integrative Biology
@texas_IB · X

RT @ut_mbs: Congratulations, @ut_mbs @TexasScience Dr. Leslie Stevens, Prof. David Stein & the Team for publication in @PLOSGenetics "Light…

Nov 9, 2021 · 2 shares · 1,419 followers View post →
PLOS Genetics
@PLOSGenetics · X

RT @ut_mbs: Congratulations, @ut_mbs @TexasScience Dr. Leslie Stevens, Prof. David Stein & the Team for publication in @PLOSGenetics "Light…

Nov 8, 2021 · 2 shares · 34,736 followers View post →
Molecular Biosciences
@ut_mbs · X

Congratulations, @ut_mbs @TexasScience Dr. Leslie Stevens, Prof. David Stein & the Team for publication in @PLOSGenetics "Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster" https://t.co/yDW6vfIQVE https://t.co/l7Npt0gLz2

Nov 8, 2021 · 11 likes · 2 shares · 689 followers View post →
Sylvia Varland
@SylviaVarland · X

RT @PLOSGenetics: Stevens et al identify and describe a new #peptide degradation signal, the photo-N-degron When fused to the N-terminus o…

Aug 12, 2021 · 4 shares · 312 followers View post →
Julie Huang
@jhuang215 · X

RT @PLOSGenetics: Stevens et al identify and describe a new #peptide degradation signal, the photo-N-degron When fused to the N-terminus o…

Aug 2, 2021 · 4 shares · 186 followers View post →
Linda
@linda_brunotte · X

RT @PLOSGenetics: Stevens et al identify and describe a new #peptide degradation signal, the photo-N-degron When fused to the N-terminus o…

Aug 2, 2021 · 4 shares · 324 followers View post →
PLOS Genetics
@PLOSGenetics · X

Stevens et al identify and describe a new #peptide degradation signal, the photo-N-degron When fused to the N-terminus of a protein, it can induce N-end rule-mediated degradation in response to blue-light illumination

Aug 1, 2021 · 12 likes · 4 shares · 34,736 followers View post →
David Solecki (pronounced so_lets_ki
@So_lets_kiLab70 · X

RT @t_koromila: Excited that our @PLOSGenetics paper is out! A new photo-N-degron that can be used in Optogenetic in vivo studies is now av…

May 26, 2021 · 5 shares · 3,987 followers View post →
PLOS Genetics
@PLOSGenetics · X

RT @DouganLab: #PLOSGenetics: Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae ... https://t.…

May 21, 2021 · 1 share · 34,736 followers View post →
Ah-Ram Kim
@ahramkim1128 · X

Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster https://t.co/rn8Jyg0j46

May 20, 2021 · 283 followers View post →
Dougan Lab
@DouganLab · X

#PLOSGenetics: Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae ... https://t.co/sR2ANgzKh4

May 20, 2021 · 2 likes · 1 share · 933 followers View post →
PLOS Genetics
@PLOSGenetics · X

RT @StefanLuschnig: #PLOSGenetics: Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae ... https…

May 19, 2021 · 2 shares · 34,736 followers View post →
PLOS Genetics
@PLOSGenetics · X

RT @stathlab: We've seen the light, and with great spatial and temporal resolution, too!

May 18, 2021 · 4 shares · 34,736 followers View post →
PLOS Genetics
@PLOSGenetics · X

RT @t_koromila: Excited that our @PLOSGenetics paper is out! A new photo-N-degron that can be used in Optogenetic in vivo studies is now av…

May 18, 2021 · 5 shares · 34,736 followers View post →
Jia
@chenjiajiachen · X

RT @stathlab: We've seen the light, and with great spatial and temporal resolution, too!

May 18, 2021 · 4 shares · 174 followers View post →
the Node
@the_Node · X

RT @stathlab: We've seen the light, and with great spatial and temporal resolution, too!

May 18, 2021 · 4 shares · 18,755 followers View post →
@luschnig.bsky.social
@StefanLuschnig · X

#PLOSGenetics: Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae ... https://t.co/htzq7kxxFM

May 18, 2021 · 2 likes · 2 shares · 1,158 followers View post →
Frank Macabenta
@FrankMacabenta · X

RT @stathlab: We've seen the light, and with great spatial and temporal resolution, too!

May 17, 2021 · 4 shares · 118 followers View post →
Stathopoulos Lab
@stathlab · X

We've seen the light, and with great spatial and temporal resolution, too!

May 17, 2021 · 22 likes · 4 shares · 171 followers View post →
Amjad Askary
@AskaryLab · X

RT @t_koromila: Excited that our @PLOSGenetics paper is out! A new photo-N-degron that can be used in Optogenetic in vivo studies is now av…

May 17, 2021 · 5 shares · 912 followers View post →
Jeremy
@Dufourt34 · X

RT @t_koromila: Excited that our @PLOSGenetics paper is out! A new photo-N-degron that can be used in Optogenetic in vivo studies is now av…

May 17, 2021 · 5 shares · 464 followers View post →
Theodora Koromila
@t_koromila · X

Excited that our @PLOSGenetics paper is out! A new photo-N-degron that can be used in Optogenetic in vivo studies is now available https://t.co/tGMu95pDFx! Fun to collaborate with the Stein lab! Also, thank you @Angela_Stathop, David Stein and James McGehee

May 17, 2021 · 14 likes · 5 shares · 295 followers View post →