Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies.

Abstract in brief

• The article discusses how fecal microbiota transplantation (FMT) is used in mouse studies to understand gut microbiome's role in disease and explores the variability in procedures used by different research groups.
• It emphasizes the importance of maintaining anaerobic conditions during sample collection and processing, as well as using methods like germ-free mice or antibiotics to prepare recipients for better microbiome engraftment.
• The review aims to identify best practices for FMT, including donor handling, dosage, administration routes, and success assessment, to help establish standardized, reproducible protocols in research.]