PubHawk
Biotechnol Bioeng June 2022 PMID 35180317

Modeling apoptosis resistance in CHO cells with CRISPR-mediated knockouts of Bak1, Bax, and Bok.

Michael A MacDonald, Craig Barry, Teddy Groves, Verónica S Martínez, Peter P Gray, Kym Baker, Evan Shave, Stephen Mahler, Trent Munro, Esteban Marcellin, Lars K Nielsen

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Mentions

19
Peak: 5 in Jun 2022
across 4 sources since Feb 2022

Extended reach

65,806
potential audience

Total likes

24

Video views

6

Mentions per month

Feb 2022 – Dec 2025

Where it's discussed

  • X / Twitter12 posts
  • Facebook3 posts
  • YouTube2 videos
  • News2 articles

AI synopsis

What people are saying

The discussions highlight the importance of the study by referencing specific techniques like Bayesian inference and genetic knockouts, with phrases like 'quantify improvements' and 'cool article I helped with' indicating active interest and appreciation. The tone is positive and collaborative, demonstrating recognition of the research's scientific and practical impact.

AgreementModerate agreement

Most discussions recognize the value and relevance of the research, indicating general agreement with its significance.

InterestHigh interest

The numerous positive reactions and calls to check out the article show high interest in the topic.

EngagementModerate engagement

Participants discuss technical aspects like modeling approaches and experimental methods, reflecting moderate engagement.

ImpactModerate impact

The focus on improving bioprocessing and cell survival suggests the research could have meaningful implications in the biopharmaceutical field.

Abstract in brief

  • The study used CRISPR-Cas9 to create CHO cell lines with knockouts of Bak1, Bax, and Bok to understand how these genes affect cell death (apoptosis) under stress, revealing that removing Bak1 and Bax delays apoptosis but deleting Bok doesn't have a significant effect.
  • The researchers developed a population-based model incorporating a delay function and death rate to accurately describe how these genetically modified cells respond to different cytotoxic treatments, allowing for better prediction of cell survival times.
  • These findings help connect genetic engineering of cell lines with biological modeling, providing insights that could improve biopharmaceutical manufacturing by increasing cell survival and product yields during bioprocessing.]
Read the full abstract

Top posts

Most liked on X and Bluesky
Craig
@craig_p_barry · X

Check out how we quantify improvements in bak1bax KO cell line survivability with a tidy new #bioprocess model, featuring some #bayesianinference for parameter estimation. Had a blast working with Michael, and learnt so much from @teddygroves https://t.co/OPTsH9cnP8

Jun 16, 2022 · 11 likes · 4 shares · 28 followers View post →
Liam Bright
@lastpositivist · X

In fact going by recent work: Howarth is in biostats (https://t.co/DRxYfmpp5q), Ronel in Infection and Immunity (https://t.co/hHh2rtSHxi), Hill is in public health (https://t.co/RhzHlqNq17), Groves in biosustainability (https://t.co/o2RD83HOfq) -- strong link to medical biology.

Dec 26, 2022 · 6 likes · 61,592 followers View post →
Teddy Groves
@teddygroves · X

Check out this cool article I helped with! https://t.co/83Vj7v4V8T

Mar 7, 2022 · 6 likes · 1 share · 241 followers View post →
CRISPRpapers
@CRISPR_Articles · X

Modelling Apoptosis Resistance in CHO cells with CRISPR-Mediated Knock-outs of Bak1, Bax, and Bok https://t.co/a6au8PhrDU https://t.co/8cCH15KX7t

Feb 18, 2022 · 1,449 followers View post →
Trent Munro
@trent_munro · X

RT @craig_p_barry: Check out how we quantify improvements in bak1bax KO cell line survivability with a tidy new #bioprocess model, featurin…

Jun 19, 2022 · 4 shares · 996 followers View post →
Marta Matos @marta_ra_matos@climatejustice.social
@marta_ra_matos · X

RT @teddygroves: Check out this cool article I helped with! https://t.co/83Vj7v4V8T

Mar 7, 2022 · 1 share · 374 followers View post →

All mentions

CHO Cell Papers
@CHOculturePaper · X

Dec 26, 2025 · 59 followers View post →
CHO Cell Papers
@CHOculturePaper · X

Sep 22, 2025 · 59 followers View post →
Liam Bright
@lastpositivist · X

In fact going by recent work: Howarth is in biostats (https://t.co/DRxYfmpp5q), Ronel in Infection and Immunity (https://t.co/hHh2rtSHxi), Hill is in public health (https://t.co/RhzHlqNq17), Groves in biosustainability (https://t.co/o2RD83HOfq) -- strong link to medical biology.

Dec 26, 2022 · 6 likes · 61,592 followers View post →
Aspen Media
@aspen_media_inc · X

Chinese hamster ovary (CHO) cells are the primary platform for the production of biopharmaceuticals. Learn More: https://t.co/s5qITTF7V3 #aspenalert https://t.co/rEqvQQmhiZ

Oct 26, 2022 · 311 followers View post →
Henry de Malmanche
@HenryDmal · X

RT @craig_p_barry: Check out how we quantify improvements in bak1bax KO cell line survivability with a tidy new #bioprocess model, featurin…

Jun 20, 2022 · 4 shares · 264 followers View post →
Trent Munro
@trent_munro · X

RT @craig_p_barry: Check out how we quantify improvements in bak1bax KO cell line survivability with a tidy new #bioprocess model, featurin…

Jun 19, 2022 · 4 shares · 996 followers View post →
CBI-ITTC
@CBI_ITTC · X

RT @craig_p_barry: Check out how we quantify improvements in bak1bax KO cell line survivability with a tidy new #bioprocess model, featurin…

Jun 19, 2022 · 4 shares · 93 followers View post →
Teddy Groves
@teddygroves · X

RT @craig_p_barry: Check out how we quantify improvements in bak1bax KO cell line survivability with a tidy new #bioprocess model, featurin…

Jun 18, 2022 · 4 shares · 241 followers View post →
Craig
@craig_p_barry · X

Check out how we quantify improvements in bak1bax KO cell line survivability with a tidy new #bioprocess model, featuring some #bayesianinference for parameter estimation. Had a blast working with Michael, and learnt so much from @teddygroves https://t.co/OPTsH9cnP8

Jun 16, 2022 · 11 likes · 4 shares · 28 followers View post →
Marta Matos @marta_ra_matos@climatejustice.social
@marta_ra_matos · X

RT @teddygroves: Check out this cool article I helped with! https://t.co/83Vj7v4V8T

Mar 7, 2022 · 1 share · 374 followers View post →
Teddy Groves
@teddygroves · X

Check out this cool article I helped with! https://t.co/83Vj7v4V8T

Mar 7, 2022 · 6 likes · 1 share · 241 followers View post →
CRISPRpapers
@CRISPR_Articles · X

Modelling Apoptosis Resistance in CHO cells with CRISPR-Mediated Knock-outs of Bak1, Bax, and Bok https://t.co/a6au8PhrDU https://t.co/8cCH15KX7t

Feb 18, 2022 · 1,449 followers View post →
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