Nat Commun April 2023 PMID 37029107

Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells.

Michelle Küppers, David Albrecht, Anna D Kashkanova, Jennifer Lühr, Vahid Sandoghdar

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Peak: 1 in Jun 2022

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The collective comments reflect a strong appreciation for the microscopy technique's ability to visualize cellular structures in 3D with high resolution, often describing it as a breakthrough ('game changer') that could significantly impact cell biology and virology research, illustrated by mentions of tracking nanoparticles and viral particles without labels. The tone and language emphasize excitement and recognition of its scientific importance.

AgreementModerate agreement

Most discussions recognize the significance of the microscopy technique and its potential for advancing live-cell imaging.

InterestHigh interest

Posts demonstrate high curiosity and enthusiasm for understanding the technology and its applications.

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The discussions include references to technical aspects and real-world implications, showing moderate depth of engagement.

ImpactHigh impact

Several posts highlight the transformative potential of this method for research, indicating a perceived high impact.

Abstract in brief

This study introduces confocal interferometric scattering (iSCAT) microscopy as a powerful label-free technique to achieve three-dimensional imaging of live cells at nanometer resolution, solving limitations of traditional brightfield microscopy in terms of 3D visualization and sensitivity to nanoscale features.
Using confocal iSCAT, researchers can visualize detailed cellular structures like the nuclear envelope, endoplasmic reticulum dynamics, and single microtubules, as well as track individual nanoparticles such as SARS-CoV-2 virions in real time, all without the need for labels or dyes.
The method can be integrated into existing laser scanning microscopes and is especially useful for live-cell studies where labeling is difficult or impossible, enabling long-term monitoring of cellular processes beyond the constraints of photobleaching in fluorescence imaging.]

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3D Nanoscopic Imaging of Live Cells with Confocal Interferometric Scattering Microscopy

Confocal interferometric scattering (iSCAT) microscopy enables label-free 3D imaging of live cells at nanometer resolution, revealing cellular structures and dynamics in real time without dyes or labels. It extends…

STEM RTCL TV · Jul 25, 2023 51 views

Proteins InVivo InVitro

@BVpapers · X

Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells: Bright-field light microscopy and related phase-sensitive techniques play an important role in life sciences because they provide facile and… https://t.co/Bc5wIi9RqI https://t.co/5VuhBdkgDY

Apr 8, 2023 · 43 followers View post →

Ryota IINO 飯野亮太

@ryotaiino · X

Read this #preprint on @researchsquare: Confocal Interferometric Scattering Microscopy Reveals 3D Nanoscopic Structure and Dynamics in Live Cells https://t.co/dnOyzAum0S

Dec 1, 2022 · 2 likes · 6,429 followers View post →

Research Square Covid-19 Preprints

@RS_COVID19 · X

Confocal Interferometric Scattering Microscopy Reveals 3D Nanoscopic Structure and Dynamics in Live Cells #covid https://t.co/i9EF2uvgQr

Jun 1, 2022 · 640 followers View post →