Biotechnol Bioeng September 2023 PMID 37039773

High-efficiency and multilocus targeted integration in CHO cells using CRISPR-mediated donor nicking and DNA repair inhibitors.

Nathaniel K Hamaker, Kelvin H Lee

Mentions

19
Peak: 4 in Nov 2025
across 5 sources since Apr 2023

Extended reach

4,039
potential audience

Total likes

4

Video views

0

Mentions per month

Apr 2023 – Nov 2025

Where it's discussed

  • Facebook10 posts
  • News4 articles
  • YouTube2 videos
  • Blogs2 articles
  • X / Twitter1 posts

AI synopsis

What people are saying

Discussions frequently highlight the innovative use of donor nicking and DNA repair inhibitors, with posts stating the method 'significantly increases targeted gene insertion' and describing it as a 'game changer' for cell line engineering. The tone is positive and forward-looking, emphasizing the potential for high-impact applications in biotechnology.

AgreementModerate agreement

Most of the discussions express strong support or enthusiasm for the research, highlighting its potential improvements in gene editing approaches.

InterestHigh interest

The majority of posts show high curiosity and positive reactions, emphasizing the novelty and practical relevance of the findings.

EngagementModerate engagement

Several discussions delve into technical aspects like donor nicking and DNA repair inhibitors, indicating a meaningful level of engagement.

ImpactHigh impact

Many posts suggest that this research could significantly influence future cell engineering practices and biopharmaceutical production.

Abstract in brief

  • This study developed a new CRISPR-based system with a superfolder orange fluorescent protein to improve gene editing precision and efficiency in Chinese hamster ovary (CHO) cells, achieving targeted and random DNA integration.
  • Using specific donor formats and Cas9 variants, the researchers significantly increased targeted gene insertion accuracy, with donor nicking reducing unwanted random integrations and enhancing overall outcomes.
  • They also identified two DNA repair inhibitors that boost the efficiency of precise gene insertion to 25% of transfected cells, leading to high success rates in multi-locus gene integration, which can advance cell line engineering and protein production.]

Top posts

Most liked on X and Bluesky
CRISPRpapers
@CRISPR_papers · X

High-efficiency and multilocus targeted integration in CHO cells using CRISPR-mediated donor nicking and DNA repair inhibitors https://t.co/XBgLmyr05A

Apr 11, 2023 · 3 likes · 3,879 followers View post →

All mentions

CRISPRpapers
@CRISPR_papers · X

High-efficiency and multilocus targeted integration in CHO cells using CRISPR-mediated donor nicking and DNA repair inhibitors https://t.co/XBgLmyr05A

Apr 11, 2023 · 3 likes · 3,879 followers View post →
Biotechnology Journal - 2025 - Szkodny - A Flexible Hybrid Site ...
News

9 (2023): 2419–2440, https://doi.org/10.1002/bit. Integration Chinese Hamster Ovary Host Directly Targeting Either 28393. One or Two Vectors Simultaneously to a ...

Dec 29, 2025 View →
Wa0009. | PDF | Antenna (Radio) | Radiation
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International Journal, 11(1), 1–4. https://doi.org/10.1002/mop.28393. Pan ... A High Aperture Efficiency 1-Bit Reconfigurable Reflectarray Antenna With Extremely ...

Dec 29, 2025 View →
Tugas Prof Irfan | PDF | Cardiac Muscle | Cell Culture
News

doi: 10.1038/s41598-018-28393-y 21. Ravenscroft SM, Pointon A, Williams AW ... Biotechnol Bioeng. (2017) 87:521. doi: 10.1002/bit.26504 37. Ulmer BM ...

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... 10.1002/mop.28393)). Concluding Remarks Quantum metamaterial ... bit reprogrammable metasurface. (a) The configuration of the proposed ...

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Read more - via Blog – Hackaday http://bit. ly/2KTdax1. Incredibly Tiny ... 10.1002/mop.28393)). Concluding Remarks Quantum metamaterial ...

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Facebook

/bit. ly/3Y3J3Yk. Natalie Chan. 2y · Public · 'Stunning' discovery ... 10.1002/mop.28393)). Concluding Remarks Quantum metamaterial ...

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Superconductor and Ferromagnetic!
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... 10.1002/mop. 28393)). Concluding Remarks Quantum metamaterial ... bit of isolation), Litz wire for all the leads, and a "Select-a ...

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PDF. Erqian Dong, Yu Zhang, Zhongchang Song, Tianye Zhang ...
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... 10.1002/mop.28393)). Concluding Remarks Quantum metamaterial ... bit reprogrammable metasurface. (a) The configuration of the proposed ...

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Metamaterials and quantum optics
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... 10.1002/mop.28393)). Concluding Remarks Quantum metamaterial ... Nanotechnology Meets Quantum Computing: Advancing Quantum Bits (Qubits) and ...

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"Scientists from Russia and the U.K. have developed an antenna ...
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... 10.1002/mop.28393)). Concluding Remarks Quantum metamaterial ... Read more - via Blog – Hackaday http://bit. ly/2KTdax1. Incredibly Tiny ...

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