Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi.

Sine Yaganoglu, Konstantinos Kalyviotis, Christina Vagena-Pantoula, Dörthe Jülich, Benjamin M Gaub, Maaike Welling, Tatiana Lopes, Dariusz Lachowski, See Swee Tang, Armando Del Rio Hernandez, Victoria Salem, Daniel J Müller, Scott A Holley, Julien Vermot, Jian Shi

July 2023 Nat Commun

Synopsis of Social media discussions

Discussions highlight the novelty and applicability of GenEPi, with expressions like 'highly specific and non-invasive' emphasizing its technological advancement, and mentions of applications in zebrafish demonstrate its broad potential in biological research. The tone is enthusiastic and recognizes the study's significance, indicating deep engagement and perceived high impact.

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Agreement
 Moderate agreement

Most discussions praise the publication’s innovative approach, as seen in comments like 'Thank you for making your #methodsmatter,' indicating strong support for the significance of the research.

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Interest
 High level of interest

The discussions generate high interest, with mentions of the technique being 'highly specific and non-invasive' and its application across biological scales, capturing attention of those interested in imaging and mechanobiology.

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Engagement
 Moderate level of engagement

Participants actively discuss the methods and implications, such as referencing transgenic zebrafish models and the potential to study mechanotransduction, showing thoughtful engagement.

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Impact
 High level of impact

The publication is viewed as a breakthrough, especially in offering new tools (

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3 Articles

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10 Articles

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9

Total Likes

50

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20,598

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Posts referencing the article

Non-Invasive Imaging of Piezo1 Activity Using GenEPi Across Biological Scales

Non-Invasive Imaging of Piezo1 Activity Using GenEPi Across Biological Scales

GenEPi is a genetically encoded fluorescent reporter enabling high-resolution, non-invasive imaging of Piezo1 ion channel activity in living tissues, from single cells to whole organisms, facilitating the study of mechanotransduction in development, homeostasis, and disease.

STEM RTCL TV

October 16, 2023

9 views

  • Marie Held
    @MaKaefer (Twitter)

    Rapidly followed by Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi presented by Periklis (Laki) Pantazis #elmi2024 https://t.co/KdrvqCOl3c
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    June 7, 2024

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  • @KKalyviotis (Twitter)

    RT @SocDevBio: .@KKalyviotis of @imperialcollege will round out the #SDBPostdocSeminar with his talk "Highly specific and non-invasive imag…
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    May 8, 2024

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  • Society for Developmental Biology
    @SocDevBio (Twitter)

    .@KKalyviotis of @imperialcollege will round out the #SDBPostdocSeminar with his talk "Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi" Image: Transgenic zebrafish expressing GenEPi, the Piezo1-dependent activity biosensor https://t.co/Gv0X3UEIbj
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    May 8, 2024

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  • RRID Robot
    @RobotRrid (Twitter)

    RRIDs were included in the Nature Communications paper "Highly specific and non-invasive imaging of Piezo1…" (https://t.co/G01GAfkC50). Thank you for making your #methodsmatter. #RRID
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    July 23, 2023

  • Yi Feng
    @YiFeng_Fishlab (Twitter)

    RT @Pantazis_Lab:
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    July 21, 2023

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  • Christina Vagena-Pantoula
    @CVagenaPantoula (Twitter)

    RT @Pantazis_Lab:
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    July 21, 2023

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  • Inyoung Jeong (Jung)
    @InyoungJeong2 (Twitter)

    RT @Pantazis_Lab:
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    July 21, 2023

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  • A del Rio Hernandez
    @adriohernandez (Twitter)

    RT @Pantazis_Lab:
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    July 20, 2023

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  • Clara Gordillo Pi
    @claragordillopi (Twitter)

    RT @Pantazis_Lab:
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    July 20, 2023

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  • Marie Breau
    @MarieBreau1 (Twitter)

    RT @Pantazis_Lab:
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    July 20, 2023

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  • @KKalyviotis (Twitter)

    RT @Pantazis_Lab:
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    July 20, 2023

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    July 20, 2023

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Abstract Synopsis

  • The text introduces GenEPi, a genetically encoded fluorescent reporter that allows for noninvasive, high-resolution imaging of Piezo1 ion channel activity across different biological scales, from single cells to whole organisms.
  • Unlike traditional methods that are invasive or indirect (such as electrophysiology or calcium imaging), GenEPi provides a real-time, optical way to monitor mechanical stimuli and Piezo1 activity in living tissues.
  • This tool can be used to study mechanotransduction processes involved in development, homeostasis, and disease by revealing how cells respond to mechanical forces in various biological contexts.]