Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration.

Abstract in brief

• This study focuses on improving acellular nerve allografts (ANAs) for peripheral nerve repair by developing an optimized multichannel design using a modified decellularization method, which helps retain critical extracellular matrix components and regenerative factors.
• The new ANAs feature microchannels with larger pores that increase porosity and swelling, improve internal structure, and make their mechanical properties more similar to natural nerves, leading to better biocompatibility and support for Schwann cell growth.
• In vivo testing showed that these optimized ANAs significantly enhance nerve regeneration, axon growth, myelination, and muscle function recovery compared to traditional ANAs, indicating their promising potential for clinical use.]