Quantitative Recoveries of Exosomes and Monoclonal Antibodies from Chinese Hamster Ovary Cell Cultures by Use of a Single, Integrated Two-Dimensional Liquid Chromatography Method.

Abstract in brief

• This study introduces a new 2D liquid chromatography method that efficiently measures both exosomes and monoclonal antibodies (mAbs) from the same sample taken from Chinese hamster ovary (CHO) cell cultures, which are commonly used to produce therapeutic proteins.
• The method uses specialized polymer fibers to capture exosomes and mAbs separately within a 10-minute process, with exosomes identified by light scattering and mAbs by protein detection, highlighting its speed and effectiveness.
• Overall, this technique demonstrates the potential to streamline the production and analysis of multiple biotherapeutics, such as exosomes and antibodies, from a single bioreactor, which could improve manufacturing efficiency in biopharmaceuticals.