Quantitative proteomics reveals cellular responses to individual mAb expression and tunicamycin in CHO cells.

Abstract in brief

• Quantitative proteomic analysis of CHO cells revealed that protein folding and UPR-related proteins are differentially expressed in high versus low antibody-producing cell lines and under tunicamycin-induced ER stress.
• Key proteins such as Hspa9, Dnaja3, Nedd8, and Lgmn are involved in stress responses and protein folding processes, indicating their potential role in enhancing antibody production.
• The study highlights that tunicamycin treatment activates the unfolded protein response at a low level, providing insights into how cellular stress mechanisms impact bioproduction in CHO cells.]