Synergistic anti-aging effect of Dendrobium officinale polysaccharide and spermidine: A metabolomics analysis focusing on the regulation of lipid, nucleotide and energy metabolism.

Abstract in brief

• * Combining DOP and Spd showed a strong synergistic effect in extending the lifespan of C. elegans, with the optimal doses being 250 mg/L DOP and 29.0 mg/L Spd, indicated by high combination indices in stress tolerance assays.
• * Metabolomic analysis revealed that these compounds significantly alter lipid metabolism, suggesting that their combined anti-aging benefits primarily result from modifications in lipid metabolic processes, further validated using a high-fat diet mouse model.