N-Glycosylation modulators for targeted manipulation of glycosylation for monoclonal antibodies.

Abstract in brief

• This study focuses on how to control the glycosylation (sugar modifications) of monoclonal antibodies (mAbs), which is important because it affects their safety, effectiveness, and how long they last in the body.
• Researchers tested 20 different media additives like metal ions, vitamins, sugars, and nucleosides to see how they influence glycosylation, and then selected 6 that had the most impact to fine-tune the glycan profile of a lab-produced antibody to match a commercial reference.
• The optimized process produced a glycosylation pattern nearly identical to the reference, without affecting other quality aspects like charge, aggregates, or cell health, demonstrating a promising way to improve antibody quality through targeted media additives.]