Curr Protoc August 2025 PMID 40843909

Preparation of Cuvette-Based Sorters for Sorting Submicron Microbial Cells and Viruses from Environmental and Biological Samples.

Jamie C Tijerina, Francisco Martinez-Hernandez, Vera Beilinson, Olivia Finney, Victoria J Orphan, Rochelle A Diamond

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Peak: 5 in Aug 2025

across 3 sources since Sep 2024

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Sep 2024 – Aug 2025

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The discussions reflect a generally positive reception with examples like congratulatory notes to the authors and sharing of the publication link, emphasizing community interest and recognition of the research's significance. The tone and choice of words like 'useful resource' and 'hope for this to be helpful' indicate a respectful and supportive attitude towards the publication.

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Posts demonstrate moderate interest, highlighting enthusiasm for sharing the research and its relevance to the scientific community.

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The comments reference specific details of the research and acknowledge the authors, showing active engagement without deep analytical discussion.

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The discussions suggest the article is seen as a beneficial resource, likely to influence further research in microbial cell sorting.

Abstract in brief

This protocol explains how to prepare the BD FACSAria III/IIIIFusion cell sorter for accurately sorting very small particles like viruses and ultramicrobacteria from environmental or biological samples, emphasizing proper cleaning and sample prep to ensure reliable results.
The guide is useful for labs using similar cuvette-based flow cytometry machines, providing a standardized workflow to enhance detection, prevent contamination, and improve downstream DNA sequencing of tiny particles.
It highlights the importance of cleaning the instrument thoroughly, preparing filtered sheath fluid without preservatives, and handling samples carefully to avoid noise, contamination, and issues that can compromise sorting efficiency and DNA analysis.]

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All mentions

Preparing Sheath Tank for Autoclaving in Flow Cytometry Operations

A demonstration of the procedure to prepare your BD FACSAria III/IIIIFusion sheath tank for autoclaving. This essential maintenance step ensures clean, reproducible small particle flow cytometry, especially for sorting…

Jamie · Sep 25, 2024 77 views

Karie Holtermann

@KarieHoltermann · X

RT @jcamthrash: Preparation of Cuvette‐Based Sorters for Sorting Submicron Microbial Cells and Viruses from Environmental and Biological Sa…

Aug 29, 2025 · 2 shares · 2,056 followers View post →

Ruiwen Hu, PhD

@MicrobeCookie · X

RT @jcamthrash: Preparation of Cuvette‐Based Sorters for Sorting Submicron Microbial Cells and Viruses from Environmental and Biological Sa…

Aug 28, 2025 · 2 shares · 113 followers View post →

Cameron Thrash

@jcamthrash.bsky.social · Bluesky

Preparation of Cuvette‐Based Sorters for Sorting Submicron Microbial Cells and Viruses from Environmental and Biological Samples currentprotocols.onlinelibrary.wiley.com/doi/10.1002/... #jcampubs

Aug 28, 2025 · 6 likes · 2 shares View post →

Cameron Thrash

@jcamthrash · X

Preparation of Cuvette‐Based Sorters for Sorting Submicron Microbial Cells and Viruses from Environmental and Biological Samples https://t.co/xvZuhUfpe7

Aug 28, 2025 · 9 likes · 2 shares · 17,488 followers View post →

Jamie C. Tijerina

@jamietijerina · X

Aug 25, 2025 · 311 followers View post →