Publications by authors named "David L Hacker"

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Large-Scale Transient Transfection of Suspension-Adapted Chinese Hamster Ovary Cells for the Production of the Trimeric SARS-CoV-2 Spike Protein.
David L Hacker, Kelvin Lau, Laurence Durrer, Soraya Quinche, Michael François

Methods Mol Biol· October 2024

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Small-Scale Cultivation and Transfection of ExpiCHO and HEK293E Cells in Single-Use Orbitally Shaken Bioreactors.
David L Hacker, Laurence Durrer, Soraya Quinche, Kelvin Lau, Florence Pojer

Methods Mol Biol· June 2024

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Large-Scale Production of Recombinant Noggin and R-Spondin1 Proteins Required for the Maintenance of Stem Cells in Intestinal Organoid Cultures.
David L Hacker, Paloma Ordóñez-Morán

Methods Mol Biol· March 2021

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Analysis of volumetric mass transfer coefficient ( ) in small- (250 mL) to large-scale (2500 L) orbitally shaken bioreactors.
Likuan Zhu, Bin Xu, Xiaoyu Wu, Jianguo Lei, David L Hacker

3 Biotech· September 2020

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CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors.
David L Hacker, Laurence Durrer, Soraya Quinche

Methods Mol Biol· May 2019

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Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension.
Yashas Rajendra, Sowmya Balasubramanian, David L Hacker

Methods Mol Biol· February 2018

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Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line.
Xiao Shen, Danijel Dojcinovic, Lucia Baldi, David L Hacker, Immanuel F Luescher

Biotechnol Lett· January 2018

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Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes.
Li-Kuan Zhu, Bo-Yan Song, Zhen-Long Wang, Dominique T Monteil, Xiao Shen, David L Hacker

Biotechnol Prog· January 2017

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Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents.
Yashas Rajendra, Sowmya Balasubramanian, Divor Kiseljak, Lucia Baldi, Florian M Wurm, David L Hacker

Biotechnol Prog· October 2016

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Multigene expression in stable CHO cell pools generated with the piggyBac transposon system.
Sowmya Balasubramanian, Florian M Wurm, David L Hacker

Biotechnol Prog· September 2016

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A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation.
Dominique T Monteil, Valentin Juvet, Jonathan Paz, Marc Moniatte, Lucia Baldi, David L Hacker

Biotechnol Prog· September 2016

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Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension.
Dominique T Monteil, Xiao Shen, Giulia Tontodonati, Lucia Baldi, David L Hacker

Anal Biochem· July 2016

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Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines.
Sowmya Balasubramanian, Yashas Rajendra, Lucia Baldi, David L Hacker, Florian M Wurm

Biotechnol Bioeng· June 2016

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Recombinant protein production from stable mammalian cell lines and pools.
David L Hacker, Sowmya Balasubramanian

Curr Opin Struct Biol· June 2016

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Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells.
Yashas Rajendra, Divor Kiseljak, Lucia Baldi, Florian M Wurm, David L Hacker

Biotechnol Prog· January 2016

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Production of active glycosylation-deficient γ-secretase complex for crystallization studies.
Andrés Ricardo López, Mitko Dimitrov, Hermeto Gerber, Virginie Braman, David L Hacker

Biotechnol Bioeng· December 2015

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A simple plasmid-based transient gene expression method using High Five cells.
Xiao Shen, Ana K Pitol, Virginie Bachmann, David L Hacker, Lucia Baldi

J Biotechnol· December 2015

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Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools.
Sowmya Balasubramanian, Mattia Matasci, Zuzana Kadlecova, Lucia Baldi, David L Hacker

J Biotechnol· April 2015

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Virus-free transient protein production in Sf9 cells.
Xiao Shen, David L Hacker, Lucia Baldi, Florian M Wurm

J Biotechnol· February 2014

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Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells.
David L Hacker, Divor Kiseljak, Yashas Rajendra, Sarah Thurnheer, Lucia Baldi

Protein Expr Purif· November 2013

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DNA delivery with hyperbranched polylysine: a comparative study with linear and dendritic polylysine.
Zuzana Kadlecova, Yashas Rajendra, Mattia Matasci, Lucia Baldi, David L Hacker

J Control Release· August 2013

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Highly efficient production of the Alzheimer's γ-secretase integral membrane protease complex by a multi-gene stable integration approach.
Jean-René Alattia, Mattia Matasci, Mitko Dimitrov, Lorène Aeschbach, Sowmya Balasubramanian, David L Hacker

Biotechnol Bioeng· July 2013

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A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells.
Patrik O Michel, Christophe Degen, Mélanie Hubert, Lucia Baldi, David L Hacker

Anal Biochem· November 2012

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Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells.
Yashas Rajendra, Divor Kiseljak, Sagar Manoli, Lucia Baldi, David L Hacker

Biotechnol Bioeng· September 2012

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Poly(ethyleneimine)-mediated large-scale transient gene expression: influence of molecular weight, polydispersity and N-propionyl groups.
Zuzana Kadlecova, Sophie Nallet, David L Hacker, Lucia Baldi, Harm-Anton Klok

Macromol Biosci· May 2012

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