Publications by authors named "Greg A Kirchenbaum"

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Gene discovery and expression analysis of the B cell receptor repertoire in the domestic ferret model.
Luke S Hebert, Whitney Pickens, Ed Satterwhite, Gabriel B Soto, Franziska M Pflaum, Greg A Kirchenbaum

Vaccine· October 2025

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SARS-CoV-2 Infection or COVID-19 mRNA Vaccination Elicits Partially Different Spike-Reactive Memory B Cell Responses in Naïve Individuals.
Lingling Yao, Noémi Becza, Georgia Stylianou, Magdalena Tary-Lehmann, Stephen M Todryk, Greg A Kirchenbaum

Vaccines (Basel)· September 2025

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Optimizing PBMC Cryopreservation and Utilization for ImmunoSpot Analysis of Antigen-Specific Memory B Cells.
Noémi Becza, Lingling Yao, Paul V Lehmann, Greg A Kirchenbaum

Vaccines (Basel)· July 2025

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Measuring Human Memory B Cells in Autoimmunity Using Enzyme-Linked ImmunoSpot.
Georgia Stylianou, Greg A Kirchenbaum, Paul V Lehmann, Simon Pearce, Stephen Todryk

Biomolecules· April 2025

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Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays.
Paul V Lehmann, Alexey Y Karulin, Noémi Becza, Lingling Yao, Zhigang Liu, Greg A Kirchenbaum

J Immunol Methods· February 2025

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The Importance of Monitoring Antigen-Specific Memory B Cells, and How ImmunoSpot Assays Are Suitable for This Task.
Greg A Kirchenbaum, Graham Pawelec, Paul V Lehmann

Cells· February 2025

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Retraction for Carter et al., "Elicitation of Protective Antibodies against a Broad Panel of H1N1 Viruses in Ferrets Preimmune to Historical H1N1 Influenza Viruses".
Donald M Carter, Christopher A Darby, Scott K Johnson, Michael A Carlock, Greg A Kirchenbaum

J Virol· July 2024

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Artificial Intelligence-Based Counting Algorithm Enables Accurate and Detailed Analysis of the Broad Spectrum of Spot Morphologies Observed in Antigen-Specific B-Cell ELISPOT and FluoroSpot Assays.
Alexey Y Karulin, Melinda Katona, Zoltán Megyesi, Greg A Kirchenbaum, Paul V Lehmann

Methods Mol Biol· March 2024

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Monitoring Memory B Cells by Next-Generation ImmunoSpot Provides Insights into Humoral Immunity that Measurements of Circulating Antibodies Do Not Reveal.
Paul V Lehmann, Zhigang Liu, Noémi Becza, Alexis V Valente, Junbo Wang, Greg A Kirchenbaum

Methods Mol Biol· March 2024

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Assessing the Affinity Spectrum of the Antigen-Specific B Cell Repertoire via ImmunoSpot.
Noémi Becza, Zhigang Liu, Jack Chepke, Xing-Huang Gao, Paul V Lehmann, Greg A Kirchenbaum

Methods Mol Biol· March 2024

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Four-Color ImmunoSpot Assays Requiring Only 1-3 mL of Blood Permit Precise Frequency Measurements of Antigen-Specific B Cells-Secreting Immunoglobulins of All Four Classes and Subclasses.
Lingling Yao, Noémi Becza, Andrea Maul-Pavicic, Jack Chepke, Greg A Kirchenbaum

Methods Mol Biol· March 2024

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Antibody Levels Poorly Reflect on the Frequency of Memory B Cells Generated following SARS-CoV-2, Seasonal Influenza, or EBV Infection.
Carla Wolf, Sebastian Köppert, Noémi Becza, Stefanie Kuerten, Greg A Kirchenbaum

Cells· November 2022

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Impaired memory B-cell recall responses in the elderly following recurrent influenza vaccination.
Rodrigo B Abreu, Greg A Kirchenbaum, Giuseppe A Sautto, Emily F Clutter, Ted M Ross

PLoS One· November 2021

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A Competitive Hemagglutination Inhibition Assay for Dissecting Functional Antibody Activity against Influenza Virus.
Greg A Kirchenbaum, Giuseppe A Sautto, Robert A Richardson, Jeffrey W Ecker, Ted M Ross

J Virol· November 2021

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Convergent antibody evolution and clonotype expansion following influenza virus vaccination.
David Forgacs, Rodrigo B Abreu, Giuseppe A Sautto, Greg A Kirchenbaum, Elliott Drabek

PLoS One· August 2021

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Deconvoluting the T Cell Response to SARS-CoV-2: Specificity Versus Chance and Cognate Cross-Reactivity.
Alexander A Lehmann, Greg A Kirchenbaum, Ting Zhang, Pedro A Reche, Paul V Lehmann

Front Immunol· July 2021

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Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays.
Sebastian Köppert, Carla Wolf, Noémi Becza, Giuseppe A Sautto, Fridolin Franke, Greg A Kirchenbaum

Cells· July 2021

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Functional T Cell Reactivity to Melanocyte Antigens Is Lost during the Progression of Malignant Melanoma, but Is Restored by Immunization.
Anna Przybyla, Alexander A Lehmann, Ting Zhang, Jacek Mackiewicz, Łukasz Galus, Greg A Kirchenbaum

Cancers (Basel)· January 2021

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Comprehensive Evaluation of the Expressed CD8+ T Cell Epitope Space Using High-Throughput Epitope Mapping.
Paul V Lehmann, Maneewan Suwansaard, Ting Zhang, Diana R Roen, Greg A Kirchenbaum

Front Immunol· August 2020

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High-Yield Expression and Purification of Recombinant Influenza Virus Proteins from Stably-Transfected Mammalian Cell Lines.
Jeffrey W Ecker, Greg A Kirchenbaum, Spencer R Pierce, Amanda L Skarlupka, Rodrigo B Abreu

Vaccines (Basel)· August 2020

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IL-21 in Conjunction with Anti-CD40 and IL-4 Constitutes a Potent Polyclonal B Cell Stimulator for Monitoring Antigen-Specific Memory B Cells.
Fridolin Franke, Greg A Kirchenbaum, Stefanie Kuerten, Paul V Lehmann

Cells· February 2020

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Preexisting subtype immunodominance shapes memory B cell recall response to influenza vaccination.
Rodrigo B Abreu, Greg A Kirchenbaum, Emily F Clutter, Giuseppe A Sautto, Ted M Ross

JCI Insight· January 2020

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A Computationally Optimized Broadly Reactive Antigen Subtype-Specific Influenza Vaccine Strategy Elicits Unique Potent Broadly Neutralizing Antibodies against Hemagglutinin.
Giuseppe A Sautto, Greg A Kirchenbaum, Rodrigo B Abreu, Jeffrey W Ecker, Spencer R Pierce

J Immunol· January 2020

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Next Generation Vaccines for Infectious Diseases.
Giuseppe A Sautto, Greg A Kirchenbaum, Roberta A Diotti, Elena Criscuolo, Francesca Ferrara

J Immunol Res· December 2019

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Elicitation of Broadly Protective Antibodies following Infection with Influenza Viruses Expressing H1N1 Computationally Optimized Broadly Reactive Hemagglutinin Antigens.
Giuseppe A Sautto, Greg A Kirchenbaum, Jeffrey W Ecker, Anne-Gaelle Bebin-Blackwell, Spencer R Pierce

Immunohorizons· August 2018

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