Publications by authors named "Jaap Broos"

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Synthesis and Antimicrobial Specificities of Halogenated Tryptophan-Containing Nisin Variants.
Chenhui Wang, Sanne Tervoort, Oscar P Kuipers, Jaap Broos

ACS Chem Biol· October 2025

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Functionalizing Nisin with a Sugar Moiety Improves Its Solubility and Results in an Altered Antibacterial Spectrum and Mode of Action.
Longcheng Guo, Oscar P Kuipers, Jaap Broos

ACS Synth Biol· September 2025

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Heterologous Expression and Characterization of Estercin A, a Class II Lanthipeptide Derived from CF016, with Antimicrobial Activity against Clinically Relevant Pathogens.
Chenhui Wang, Joseph Wambui, Maria Victoria Fernandez-Cantos, Simon Jurt, Jaap Broos

J Nat Prod· February 2025

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An Engineered Nisin Analogue with a Hydrophobic Moiety Attached at Position 17 Selectively Inhibits Strains.
Longcheng Guo, Oscar P Kuipers, Jaap Broos

ACS Chem Biol· September 2024

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Facile Halogenation of Antimicrobial Peptides As Demonstrated by Producing Bromotryptophan-Labeled Nisin Variants with Enhanced Antimicrobial Activity.
Longcheng Guo, Oscar P Kuipers, Jaap Broos

J Nat Prod· June 2024

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Engineering hybrid lantibiotics yields the highly stable and bacteriocidal peptide cerocin V.
Longcheng Guo, Konstantin Stoffels, Jaap Broos, Oscar P Kuipers

Microbiol Res· May 2024

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Altering Specificity and Enhancing Stability of the Antimicrobial Peptides Nisin and Rombocin through Dehydrated Amino Acid Residue Engineering.
Longcheng Guo, Konstantin Stoffels, Jaap Broos, Oscar P Kuipers

Peptides· April 2024

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Rombocin, a Short Stable Natural Nisin Variant, Displays Selective Antimicrobial Activity against and Employs a Dual Mode of Action to Kill Target Bacterial Strains.
Longcheng Guo, Joseph Wambui, Chenhui Wang, Jaap Broos, Roger Stephan

ACS Synth Biol· January 2024

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Cesin, a short natural variant of nisin, displays potent antimicrobial activity against major pathogens despite lacking two C-terminal macrocycles.
Longcheng Guo, Joseph Wambui, Chenhui Wang, Francis Muchaamba, Maria Victoria Fernandez-Cantos, Jaap Broos

Microbiol Spectr· September 2023

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Lipidated variants of the antimicrobial peptide nisin produced via incorporation of methionine analogs for click chemistry show improved bioactivity.
Longcheng Guo, Chenhui Wang, Jaap Broos, Oscar P Kuipers

J Biol Chem· July 2023

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The Role of Tryptophan in π Interactions in Proteins: An Experimental Approach.
Jinfeng Shao, Bastiaan P Kuiper, Andy-Mark W H Thunnissen, Robbert H Cool, Liang Zhou, Jaap Broos

J Am Chem Soc· August 2022

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Probing the Conformational States of Thimet Oligopeptidase in Solution.
Marcelo F M Marcondes, Gabriel S Santos, Fellipe Bronze, Mauricio F M Machado, Kátia R Perez, Jaap Broos

Int J Mol Sci· June 2022

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On the efficient bio-incorporation of 5-hydroxy-tryptophan in recombinant proteins expressed in Escherichia coli with T7 RNA polymerase-based vectors.
Wellington P Oliveira-Souza, Fellipe Bronze, Jaap Broos, Marcelo F M Marcondes, Vitor Oliveira

Biochem Biophys Res Commun· October 2017

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Development of Chemically Defined Media to Express Trp-Analog-Labeled Proteins in a Lactococcus lactis Trp Auxotroph.
Jinfeng Shao, Marcelo F M Marcondes, Vitor Oliveira, Jaap Broos

J Mol Microbiol Biotechnol· May 2017

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Incorporation of tryptophan analogues into the lantibiotic nisin.
Liang Zhou, Jinfeng Shao, Qian Li, Auke J van Heel, Marcel P de Vries, Jaap Broos

Amino Acids· May 2016

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Picosecond fluorescence dynamics of tryptophan and 5-fluorotryptophan in monellin: slow water-protein relaxation unmasked.
Jianhua Xu, Binbin Chen, Patrik Callis, Pedro L Muiño, Henriëtte Rozeboom, Jaap Broos

J Phys Chem B· March 2015

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Biosynthetic incorporation of the azulene moiety in proteins with high efficiency.
Jinfeng Shao, Ivan V Korendovych, Jaap Broos

Amino Acids· January 2015

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Isolation of Escherichia coli mannitol permease, EIImtl, trapped in amphipol A8-35 and fluorescein-labeled A8-35.
Milena Opačić, Fabrice Giusti, Jean-Luc Popot, Jaap Broos

J Membr Biol· October 2014

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Biosynthetic incorporation of tryptophan analogs in proteins.
Jaap Broos

Methods Mol Biol· May 2014

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Emitting state of 5-hydroxyindole, 5-hydroxytryptophan, and 5-hydroxytryptophan incorporated in proteins.
Dejan M Petrović, Ben H Hesp, Jaap Broos

J Phys Chem B· September 2013

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An expression system for the efficient incorporation of an expanded set of tryptophan analogues.
Dejan M Petrović, Kees Leenhouts, Maarten L van Roosmalen, Jaap Broos

Amino Acids· May 2013

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Monitoring lysin motif-ligand interactions via tryptophan analog fluorescence spectroscopy.
Dejan M Petrović, Kees Leenhouts, Maarten L van Roosmalen, Fenneke Kleinjan, Jaap Broos

Anal Biochem· September 2012

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Structural investigation of the transmembrane C domain of the mannitol permease from Escherichia coli using 5-FTrp fluorescence spectroscopy.
Milena Opačić, Ben H Hesp, Fabrizia Fusetti, Bauke W Dijkstra, Jaap Broos

Biochim Biophys Acta· March 2012

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Localization of the substrate-binding site in the homodimeric mannitol transporter, EIImtl, of Escherichia coli.
Milena Opacić, Erwin P P Vos, Ben H Hesp, Jaap Broos

J Biol Chem· August 2010

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Structure of the cytoplasmic loop between putative helices II and III of the mannitol permease of Escherichia coli: a tryptophan and 5-fluorotryptophan spectroscopy study.
Erwin P P Vos, Marcel Bokhove, Ben H Hesp, Jaap Broos

Biochemistry· June 2009

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