Publications by authors named "Jason D Limberis"

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CountFU: Ditching CFUs for qPCR enumeration of Mycobacterium tuberculosis and a call to join the revolution.
Jason D Limberis, Alina Nalyvayko, Alexander Mohapatra, Rania Bouzeyen, Zach Howard

Tuberculosis (Edinb)· December 2025

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Impaired IFNγ responsiveness of monocyte-derived lung cells limits immunity to .
Weihao Zheng, Jason D Limberis, Zachary P Howard, Alex Mohapatra, Enzo Takagi

bioRxiv· November 2025

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Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow.
Jason D Limberis, Alina Nalyvayko, Janré Steyn, Jennifer Williams, Melanie Grobbelaar

J Vis Exp· June 2025

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URAdime - a tool for analyzing primer sequences in sequencing data to identify dimers and super-amplicons.
Jason D Limberis, John Z Metcalfe

bioRxiv· January 2025

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stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing.
Jason D Limberis, Roland J Nagel, Soumitesh Chakravorty, Dena Block, Scott Dewell

PLoS One· December 2024

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spotPCR: A Rapid and Efficient Approach for Indexing Individual Template Molecules using Unique Molecular Identifiers.
Jason D Limberis, Roland J Nagel, Soumitesh Chakravorty, Dena Block, Scot Dewell

bioRxiv· December 2024

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Turbolysis: A low-cost, small footprint alternative to commercial bead beaters for cell lysis.
Jason D Limberis, John Z Metcalfe

HardwareX· September 2024

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primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCR.
Jason D Limberis, John Z Metcalfe

BMC Bioinformatics· December 2023

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selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing.
Jason D Limberis, Alina Nalyvayko, Joel D Ernst, John Z Metcalfe

PLoS One· November 2023

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A simple, single-tube overlapping amplicon-targeted Illumina sequencing assay.
Jason D Limberis, Alina Nalyvayko, Joel D Ernst, John Z Metcalfe

PLoS One· September 2023

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primerJinn - a tool for rationally designing multiplex PCR primer sets and in silico PCR.
Jason D Limberis, John Z Metcalfe

Res Sq· June 2023

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Circularization of for Genotypic Bedaquiline Resistance Testing of Mycobacterium tuberculosis.
Jason D Limberis, Alina Nalyvayko, Joel D Ernst, John Z Metcalfe

Microbiol Spectr· March 2023

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Outcomes, infectiousness, and transmission dynamics of patients with extensively drug-resistant tuberculosis and home-discharged patients with programmatically incurable tuberculosis: a prospective cohort study.
Keertan Dheda, Jason D Limberis, Elize Pietersen, Jody Phelan, Aliasgar Esmail

Lancet Respir Med· April 2017

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