Publications by authors named "Kazuhito Fujiyama"

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CRISPR/Cas9-Mediated Knockouts of the ALG3 and GNTI in N. benthamiana and Their Application to Pharmaceutical Production.
Dolgormaa Bataa, Hiroyuki Kajiura, Reimi Lai Sang Sawada-Choi, Yukino Yamashita, Takeshi Ishimizu, Kazuhito Fujiyama

Plant Biotechnol J· December 2025

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Protein-Derived Signal Peptides Induced by Agrobacterium Infection Promote the Secretion of Recombinant Proteins in Nicotiana benthamiana.
Hiroyuki Kajiura, Kana Yamamoto, Ryo Misaki, Kazuhito Fujiyama

Plant Biotechnol J· December 2025

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Production and purification of tag-free recombinant human acid sphingomyelinase in Nicotiana benthamiana.
Kulachatr Panyawechamontri, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

Plant Cell Rep· October 2025

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In vitro and in silico analyses of amino acid substitution effects at the conserved N-linked glycosylation site in hepatitis B virus surface protein on antigenicity, immunogenicity, HBV replication and secretion.
Patcharin Tepjanta, Thammakorn Saethang, Kazuhito Fujiyama, Ryo Misaki, Ingorn Kimkong

PLoS One· January 2025

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Optimization of the culture medium for an iron-sensitive oleaginous yeast, Rhodotorula toruloides NBRC 0559, through functional iron deficiency.
Minsung Kim, Yuri Tanaka, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

FEMS Yeast Res· January 2025

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Absolute quantification of eight human milk oligosaccharides in breast milk to evaluate their concentration profiles and associations with infants' neurodevelopmental outcomes.
Keigo Sato, Yoshitaka Nakamura, Kazuhito Fujiyama, Kinuko Ohneda, Takahiro Nobukuni

J Food Sci· December 2024

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Recent advances in plant-based bioproduction.
Kazuhito Fujiyama, Toshiya Muranaka, Atsushi Okazawa, Hikaru Seki, Goro Taguchi

J Biosci Bioeng· July 2024

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Expression of an endo-rhamnogalacturonase from Aspergillus aculeatus enhances release of Arabidopsis transparent mucilage.
Takao Ohashi, Yurika Mabira, Yutaro Mitsuyoshi, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

J Biosci Bioeng· July 2024

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The N-linked glycosylation modifications in the hepatitis B surface protein impact cellular autophagy, HBV replication, and HBV secretion.
Patcharin Tepjanta, Kazuhito Fujiyama, Ryo Misaki, Ingorn Kimkong

PLoS One· March 2024

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Reconstitution of (1→3)-β-D-glucans measurement system using recombinant Limulus polyphemus Factor G.
Yotaro Yamamoto, Hiroyuki Kajiura, Hiroki Fukuchi, Takahiro Nishibu, Kazuhito Fujiyama

Appl Microbiol Biotechnol· December 2023

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High accumulation of the ManGlcNAc structure by combining N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1.
Keigo Sato, Hitomi Yumioka, Junko Isoyama, Koji Dohi, Akihiro Yamanaka, Kazuhito Fujiyama

J Biosci Bioeng· August 2023

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Production and -glycan engineering of Varlilumab in .
Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama

Front Plant Sci· August 2023

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Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells.
John Benson D Choa, Tadahiro Sasaki, Hiroyuki Kajiura, Kazuyoshi Ikuta, Kazuhito Fujiyama

Cytotechnology· June 2023

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Current advances in alteration of fatty acid profile in Rhodotorula toruloides: a mini-review.
Chih-Chan Wu, Kohsuke Honda, Fujiyama Kazuhito

World J Microbiol Biotechnol· June 2023

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Impact of glycoengineering and antidrug antibodies on the anticancer activity of a plant-made lectin-Fc fusion protein.
Matthew Dent, Katarina L Mayer, Noel Verjan Garcia, Haixun Guo, Hiroyuki Kajiura, Kazuhito Fujiyama

Plant Biotechnol J· November 2022

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Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression.
Yotaro Yamamoto, Hiroyuki Kajiura, Takahiro Nishibu, Kazuhito Fujiyama

J Biosci Bioeng· November 2022

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Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems.
Hiroyuki Kajiura, Ken-Ichiro Tatematsu, Tsuyoshi Nomura, Mitsuhiro Miyazawa, Akihiro Usami, Kazuhito Fujiyama

Sci Rep· November 2022

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High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in leaves.
Edjohn Aaron Macauyag, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Plant Biotechnol (Tokyo)· September 2022

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Temporal analysis of N-acetylglucosamine extension of N-glycans in the middle silk gland of silkworm Bombyx mori.
Hiroyuki Kajiura, Takanori Eguchi, Keiro Uchino, Ken-Ichiro Tatematsu, Toshiki Tamura, Kazuhito Fujiyama

J Biosci Bioeng· June 2022

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Production of recombinant β-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles.
Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

J Biosci Bioeng· May 2022

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Effect of fruit maturation on N-glycosylation of plant-derived native and recombinant miraculin.
Hiroyuki Kajiura, Kyoko Hiwasa-Tanase, Hiroshi Ezura, Kazuhito Fujiyama

Plant Physiol Biochem· May 2022

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Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter.
Ryo Misaki, Masashi Iwasaki, Hiroki Takechi, Noriko Yamano-Adachi, Takao Ohashi, Kazuhito Fujiyama

Cytotechnology· February 2022

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Biochemical characterization of Arabidopsis clade F polygalacturonase shows a substrate preference toward oligogalacturonic acids.
Takao Ohashi, Nabilah Sari, Ryo Misaki, Kazuhito Fujiyama

J Biosci Bioeng· January 2022

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Improved assay system for acidic peptide: N-glycanase (aPNGase) activity in plant extracts.
Chiharu Yamamoto, Mikako Ogura, Ryota Uemura, Maeda Megumi, Hiroyuki Kajiura, Kazuhito Fujiyama

Anal Biochem· December 2021

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Analysis of -glycan profile of Arabidopsis cell culture.
Ratna Sariyatun, Hiroyuki Kajiura, Juthamard Limkul, Ryo Misaki, Kazuhito Fujiyama

Plant Biotechnol (Tokyo)· December 2021

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