Publications by authors named "Koki Makabe"

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Regulating the orientation of homobivalent small binders through 3D domain-swapping design.
Shota Shiga, Hideki Watanabe, Koki Makabe, Shinya Honda

J Biol Chem· December 2025

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Bispecific antibody-antigen complex structures reveal activity enhancement by domain rearrangement.
Kyohei Sato, Shiro Uehara, Atsushi Tsugita, Mayuka Ishii, Shieru Ishiyama, Koki Makabe

Cell Rep· July 2025

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Evaluation of an anti-CD3 VHH and construction of an anti-CD3/anti-EGFR bispecific tandem VHH as a cancer cell targeting drug construct.
Takuya Asanuma, Peiwei Ding, Yuki Kato, Takeshi Nakanishi, Ryutaro Asano, Koki Makabe

Biochem Biophys Rep· June 2025

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Crystal structure of GH71 α-1,3-glucanase Agn1p from Schizosaccharomyces pombe: an enzyme regulating cell division in fission yeast.
Yui Horaguchi, Honoka Saitoh, Hiroyuki Konno, Koki Makabe, Shigekazu Yano

Biochem Biophys Res Commun· June 2025

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Unfolding and refolding of GH19 chitinase Chi19MK with antifungal activity from Lysobacter sp. MK9-1 at low pH and high temperature.
Moe Yokomichi, Haruki Kanno, Hiroyuki Konno, Koki Makabe, Shigekazu Yano

J Biosci Bioeng· March 2025

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Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units.
Jyunna Yoshida, Yuki Kato, Ai Isogawa, Yoshikazu Tanaka, Izumi Kumagai, Koki Makabe

J Biol Eng· October 2024

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Addition of α-1,3-glucan-binding domains to α-1,3-glucanase Agn1p from　 Schizosaccharomyces pombe enhances hydrolytic activity of insoluble α-1,3-glucan.
Yui Horaguchi, Moe Yokomichi, Masaki Takahashi, Fusheng Xu, Hiroyuki Konno, Koki Makabe

J Gen Appl Microbiol· September 2024

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Domain structure and function of α-1,3-glucanase Agl-EK14 from the gram-negative bacterium Flavobacterium sp. EK-14.
Masaki Takahashi, Moe Yokomichi, Yuki Takei, Yui Horaguchi, Koki Makabe

J Biosci Bioeng· August 2024

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Synthesis and evaluation of catecholamine derivatives as amyloid-beta aggregation inhibitors.
Fusheng Xu, Yuya Takiguchi, Koki Makabe, Hiroyuki Konno

Bioorg Med Chem Lett· July 2024

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Redesign of a thioflavin-T-binding protein with a flat β-sheet to evaluate a thioflavin-T-derived photocatalyst with enhanced affinity.
Yuina Miura, Sae Namioka, Atsushi Iwai, Norio Yoshida, Hiroyuki Konno, Koki Makabe

Int J Biol Macromol· June 2024

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SpyTag Peptide with Alkoxyl Aspartic Acids for pH-Dependent Activation of the SpyCatcher/Tag System.
Sonji Che, Hiroyuki Konno, Koki Makabe

Bioconjug Chem· May 2024

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Aspergillus oryzae α-l-rhamnosidase: Crystal structure and insight into the substrate specificity.
Koki Makabe, Naoki Ishida, Nanako Kanezaki, Yoshihito Shiono, Takuya Koseki

Proteins· February 2024

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β-Strand-mediated Domain-swapping in the Absence of Hydrophobic Core Repacking.
Mikoto Kiya, Shota Shiga, Peiwei Ding, Shohei Koide, Koki Makabe

J Mol Biol· January 2024

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α-1,3-Glucanase from the gram-negative bacterium Flavobacterium sp. EK-14 hydrolyzes fungal cell wall α-1,3-glucan.
Masaki Takahashi, Shigekazu Yano, Yui Horaguchi, Yuitsu Otsuka, Wasana Suyotha, Koki Makabe

Sci Rep· December 2023

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Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies.
Atsushi Maejima, Saori Suzuki, Koki Makabe, Izumi Kumagai, Ryutaro Asano

N Biotechnol· November 2023

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Heterologous expression of α-1,3-glucanase Agn1p from Schizosaccharomyces pombe, and efficient production of nigero-oligosaccharides by enzymatic hydrolysis from solubilized α-1,3;1,6-glucan.
Yui Horaguchi, Masaki Takahashi, Keigo Takamatsu, Hiroyuki Konno, Koki Makabe

Biosci Biotechnol Biochem· September 2023

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Construction of IgG-Fab bispecific antibody via intein-mediated protein trans-splicing reaction.
Risa Yamada, Ishin Nakahara, Izumi Kumagai, Ryutaro Asano, Takeshi Nakanishi, Koki Makabe

Sci Rep· September 2023

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Cloning and structural basis of fluorescent protein color variants from identical species of sea anemone, Diadumene lineata.
Yuki Horiuchi, Koki Makabe, Danai Laskaratou, Kuniyuki Hatori, Michel Sliwa

Photochem Photobiol Sci· July 2023

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Mutational analysis of the effects of N-glycosylation sites on the activity and thermal stability of rutinosidase from Aspergillus oryzae.
Takuya Koseki, Naoki Ishida, Ruka Hirota, Yoshihito Shiono, Koki Makabe

Enzyme Microb Technol· November 2022

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GH-16 Type β-1,3-Glucanase from sp. MK9-1 Enhances Antifungal Activity of GH-19 Type Chitinase, and Its Glucan-binding Domain Binds to Fungal Cell-wall.
Yuitsu Otsuka, Koki Sato, Shigekazu Yano, Haruki Kanno, Wasana Suyotha, Koki Makabe

J Appl Glycosci (1999)· August 2022

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Surface Engineering of Top7 to Facilitate Structure Determination.
Yuki Ito, Takuya Araki, Shota Shiga, Hiroyuki Konno, Koki Makabe

Int J Mol Sci· January 2022

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Structural analysis of the β-sheet edge of peptide self-assembly using a model protein.
Shota Shiga, Koki Makabe

Proteins· July 2021

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Cloning, expression, and characterization of a GH 19-type chitinase with antifungal activity from Lysobacter sp. MK9-1.
Shigekazu Yano, Haruki Kanno, Haruna Tsuhako, Sonoka Ogasawara, Wasana Suyotha, Koki Makabe

J Biosci Bioeng· April 2021

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The total synthesis of berberine and selected analogues, and their evaluation as amyloid beta aggregation inhibitors.
Misato Tajiri, Ryo Yamada, Mayumi Hotsumi, Koki Makabe, Hiroyuki Konno

Eur J Med Chem· April 2021

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Anti-EGFR antibody 528 binds to domain III of EGFR at a site shifted from the cetuximab epitope.
Koki Makabe, Takeshi Yokoyama, Shiro Uehara, Tomomi Uchikubo-Kamo, Mikako Shirouzu

Sci Rep· March 2021

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