Publications by authors named "Mitsuhiro Itaya"

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Versatile Methodology for Efficient Large-sized DNA Delivery Between Microorganisms Without In vitro Purification.
Shinya Kaneko, Hiromi Fukushima, Misako Nakahama, Kenji Tsuge, Jun Ishii, Mitsuhiro Itaya

J Mol Biol· September 2025

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Development of a Bacillus subtilis genome vector system that can transmit synthesized genomes.
Mitsuhiro Itaya

Biosci Biotechnol Biochem· February 2025

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Integrated conjugal plasmid pLS20 in the Bacillus subtilis genome produced 850-kbp circular subgenomes transmissible to another B. subtilis.
Mitsuhiro Itaya, Masakazu Kataoka

Genes Cells· July 2024

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Effective plasmid delivery to a plasmid-free Bacillus natto strain by a conjugational transfer system.
Mitsuhiro Itaya, Mitsuru Sato, Satoru Watanabe, Masakazu Kataoka

J Biochem· October 2022

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Bacillus subtilis 168 as a unique platform enabling synthesis and dissemination of genomes.
Mitsuhiro Itaya

J Gen Appl Microbiol· September 2022

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Heterologous production of novel and rare C-carotenoids using Planococcus carotenoid biosynthesis genes.
Miho Takemura, Chiharu Takagi, Mayuri Aikawa, Kanaho Araki, Seon-Kang Choi, Mitsuhiro Itaya

Microb Cell Fact· October 2021

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Optimization of RK2-based gene introduction system for Bacillus subtilis.
Takahiro Yokoi, Mitsuhiro Itaya, Hirotada Mori, Masakazu Kataoka

J Gen Appl Microbiol· December 2019

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Stable mutants of restriction-deficient/modification-proficient Bacillus subtilis 168: hub strains for giant DNA engineering.
Mitsuhiro Itaya, Mitsuru Sato, Satoru Watanabe, Hirofumi Yoshikawa, Masaru Tomita

J Biochem· September 2019

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Stable and efficient delivery of DNA to Bacillus subtilis (natto) using pLS20 conjugational transfer plasmids.
Mitsuhiro Itaya, Mayumi Nagasaku, Tomoe Shimada, Naoto Ohtani, Yuh Shiwa

FEMS Microbiol Lett· February 2019

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Efficient delivery of large DNA from Escherichia coli to Synechococcus elongatus PCC7942 by broad-host-range conjugal plasmid pUB307.
Mitsuhiro Itaya, Hiroko Kusakabe, Mitsuru Sato, Masaru Tomita, Rintaro Sato

J Biochem· July 2018

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Far rapid synthesis of giant DNA in the Bacillus subtilis genome by a conjugation transfer system.
Mitsuhiro Itaya, Mitsuru Sato, Miki Hasegawa, Nobuaki Kono, Masaru Tomita

Sci Rep· June 2018

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DNA synthesis by fragment assembly using extra-cellular DNA delivered by artificial controlled horizontal transfer.
Shinya Kaneko, Hiromi Fukushima, Misako Nakahama, Satomi Asano, Yasumasa Miyazaki, Mitsuhiro Itaya

J Biochem· April 2018

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The first high frequency of recombination-like conjugal transfer from an integrated origin of transfer sequence in Bacillus subtilis 168.
Mitsuhiro Itaya, Miki Hasegawa, Masaru Tomita, Mitsuru Sato

Biosci Biotechnol Biochem· February 2018

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Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain.
Naoto Ohtani, Masaru Tomita, Mitsuhiro Itaya

Appl Environ Microbiol· December 2015

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Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments.
Kenji Tsuge, Yukari Sato, Yuka Kobayashi, Maiko Gondo, Masako Hasebe, Mitsuhiro Itaya

Sci Rep· May 2015

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An inducible recA expression Bacillus subtilis genome vector for stable manipulation of large DNA fragments.
Takafumi Ogawa, Tetsuo Iwata, Shinya Kaneko, Mitsuhiro Itaya, Junji Hirota

BMC Genomics· March 2015

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Undesigned selection for replication termination of bacterial chromosomes.
Nobuaki Kono, Kazuharu Arakawa, Mitsuru Sato, Hirofumi Yoshikawa, Masaru Tomita, Mitsuhiro Itaya

J Mol Biol· August 2014

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Identification of a replication initiation protein of the pVV8 plasmid from Thermus thermophilus HB8.
Naoto Ohtani, Masaru Tomita, Mitsuhiro Itaya

Extremophiles· January 2013

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A simple method to provide a shuttling plasmid for delivery to other host ascertained by prolonged stability of extracellular plasmid DNA released from Escherichia coli K12 endA mutant, deficient in major endonuclease.
Mitsuhiro Itaya, Yoko Kawata, Mitsuru Sato, Masaru Tomita, Kenji Nakahigashi

J Biochem· December 2012

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Complete sequence of the first chimera genome constructed by cloning the whole genome of Synechocystis strain PCC6803 into the Bacillus subtilis 168 genome.
Satoru Watanabe, Yuh Shiwa, Mitsuhiro Itaya, Hirofumi Yoshikawa

J Bacteriol· December 2012

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Coupling of σG activation to completion of engulfment during sporulation of Bacillus subtilis survives large perturbations to DNA translocation and replication.
Genevieve Regan, Mitsuhiro Itaya, Patrick J Piggot

J Bacteriol· November 2012

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Serial assembly of Thermus megaplasmid DNA in the genome of Bacillus subtilis 168: a BAC-based domino method applied to DNA with a high GC content.
Naoto Ohtani, Miki Hasegawa, Mitsuru Sato, Masaru Tomita, Shinya Kaneko, Mitsuhiro Itaya

Biotechnol J· July 2012

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Sequential insertion of multiple I-SceI recognition sites at designed loci of the Bacillus subtilis 168 genome.
Mitsuhiro Itaya

Biosci Biotechnol Biochem· May 2012

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Rearrangement of gene order in the phaCAB operon leads to effective production of ultrahigh-molecular-weight poly[(R)-3-hydroxybutyrate] in genetically engineered Escherichia coli.
Ayaka Hiroe, Kenji Tsuge, Christopher T Nomura, Mitsuhiro Itaya, Takeharu Tsuge

Appl Environ Microbiol· May 2012

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The third plasmid pVV8 from Thermus thermophilus HB8: isolation, characterization, and sequence determination.
Naoto Ohtani, Masaru Tomita, Mitsuhiro Itaya

Extremophiles· March 2012

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