Publications by authors named "Tomoko Akutsu"

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Metagenomic sequencing of CRISPRs as a new marker to aid in personal identification with low-biomass samples.
Kochi Toyomane, Yuri Kimura, Takashi Fukagawa, Takayuki Yamagishi, Ken Watanabe, Tomoko Akutsu

mSystems· November 2024

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Quantitative evaluation of endometrium-expressed mRNAs for the purpose of discriminating between menstruation and traumatic vaginal injury in sexual assault cases.
Tomoko Akutsu, Saki Minegishi, Ken Watanabe, Kochi Toyomane, Takayuki Yamagishi

Int J Legal Med· July 2024

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Development and comparison of forensic interval age prediction models by statistical and machine learning methods based on the methylation rates of ELOVL2 in blood DNA.
Takayuki Yamagishi, Wataru Sakurai, Ken Watanabe, Kochi Toyomane, Tomoko Akutsu

Forensic Sci Int Genet· March 2024

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Potential application of Staphylococcus species detection in the specific identification of saliva.
Kochi Toyomane, Tomoko Akutsu, Ken Watanabe, Takayuki Yamagishi, Satoshi Kubota

Leg Med (Tokyo)· November 2023

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Validation of a novel fluorescent probe-based real-time PCR assay to detect saliva-specific unmethylated CpG sites for saliva identification.
Ken Watanabe, Takayuki Yamagishi, Kochi Toyomane, Tomoko Akutsu

Leg Med (Tokyo)· July 2023

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Precise and comprehensive determination of multiple body fluids by applying statistical cutoff values to a multiplex reverse transcription-PCR and capillary electrophoresis procedure for forensic purposes.
Tomoko Akutsu, Isao Yokota, Ken Watanabe, Kochi Toyomane, Takayuki Yamagishi

Leg Med (Tokyo)· September 2022

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Evaluation and simultaneous determination of rectal mucosa markers by multiplex reverse transcription-PCR for biological evidence of sexual assault with anal penetration.
Tomoko Akutsu, Hisako Saito, Ken Watanabe, Kochi Toyomane, Takayuki Yamagishi

Forensic Sci Int Genet· July 2022

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A new approach for forensic analysis of saliva-containing body fluid mixtures based on SNPs and methylation patterns of nearby CpGs.
Ken Watanabe, Kei Taniguchi, Kochi Toyomane, Tomoko Akutsu

Forensic Sci Int Genet· January 2022

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A vertebrate-specific qPCR assay as an endogenous internal control for robust species identification.
Kei Taniguchi, Tomoko Akutsu, Ken Watanabe, Yoshinori Ogawa, Kazuhiko Imaizumi

Forensic Sci Int Genet· January 2022

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Evaluation of CRISPR Diversity in the Human Skin Microbiome for Personal Identification.
Kochi Toyomane, Ryo Yokota, Ken Watanabe, Tomoko Akutsu, Ai Asahi

mSystems· February 2021

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Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid.
Koichi Sakurada, Ken Watanabe, Tomoko Akutsu

Diagnostics (Basel)· September 2020

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Development of a multiplex RT-PCR assay and statistical evaluation of its use in forensic identification of vaginal fluid.
Tomoko Akutsu, Isao Yokota, Ken Watanabe, Koichi Sakurada

Leg Med (Tokyo)· July 2020

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A Proposed Procedure for Discriminating between Nasal Secretion and Saliva by RT-qPCR.
Tomoko Akutsu, Ken Watanabe

Diagnostics (Basel)· July 2020

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Evaluation of a co-extraction kit for mRNA, miRNA and DNA methylation-based body fluid identification.
Ken Watanabe, Tomoko Akutsu

Leg Med (Tokyo)· February 2020

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Immunohistochemical staining of skin-expressed proteins to identify exfoliated epidermal cells for forensic purposes.
Tomoko Akutsu, Hiroshi Ikegaya, Ken Watanabe, Sachio Miyasaka

Forensic Sci Int· October 2019

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Development of a DNA methylation-based semen-specific SNP typing method: A new approach for genotyping from a mixture of body fluids.
Ken Watanabe, Kei Taniguchi, Tomoko Akutsu

Forensic Sci Int Genet· November 2018

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Evaluation of skin- or sweat-characteristic mRNAs for inferring the human origin of touched contact traces.
Tomoko Akutsu, Ken Watanabe, Ayari Takamura, Koichi Sakurada

Leg Med (Tokyo)· May 2018

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Soft and Robust Identification of Body Fluid Using Fourier Transform Infrared Spectroscopy and Chemometric Strategies for Forensic Analysis.
Ayari Takamura, Ken Watanabe, Tomoko Akutsu, Takeaki Ozawa

Sci Rep· May 2018

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Practical evaluation of an RNA-based saliva identification method.
Ken Watanabe, Tomoko Akutsu, Ayari Takamura, Koichi Sakurada

Sci Justice· November 2017

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Spectral Mining for Discriminating Blood Origins in the Presence of Substrate Interference via Attenuated Total Reflection Fourier Transform Infrared Spectroscopy: Postmortem or Antemortem Blood?
Ayari Takamura, Ken Watanabe, Tomoko Akutsu, Hiroshi Ikegaya, Takeaki Ozawa

Anal Chem· September 2017

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Advanced forensic validation for human spermatozoa identification using SPERM HY-LITER™ Express with quantitative image analysis.
Ayari Takamura, Ken Watanabe, Tomoko Akutsu

Int J Legal Med· July 2017

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The applicability of ELISA detection of gastric mucosa-expressing proteins for the identification of vomit.
Tomoko Akutsu, Hisako Saito, Hirotaro Iwase, Ken Watanabe, Ayari Takamura

Int J Legal Med· March 2017

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Development of a quantitative validation method for forensic investigation of human spermatozoa using a commercial fluorescence staining kit (SPERM HY-LITER™ Express).
Ayari Takamura, Ken Watanabe, Tomoko Akutsu

Int J Legal Med· November 2016

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Evaluation of a blood-specific DNA methylated region and trial for allele-specific blood identification from mixed body fluid DNA.
Ken Watanabe, Tomoko Akutsu, Ayari Takamura, Koichi Sakurada

Leg Med (Tokyo)· September 2016

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Development of a Real-Time PCR-Based Method for Analyzing Semen-Specific Unmethylated DNA Regions and Methylation Status in Aged Body Fluid Stains.
Ken Watanabe, Tomoko Akutsu, Koichi Sakurada

J Forensic Sci· January 2016

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