Publications by authors named "Wayne Barnes"

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Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors.
Milko B Kermekchiev, Zhian Zhang, Wayne M Barnes

Front Bioeng Biotechnol· September 2025

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A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement.
Wayne M Barnes, Zhian Zhang, Milko B Kermekchiev

Front Bioeng Biotechnol· January 2021

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Knowledge, attitudes and practice for Ebola virus disease in the national rapidly deployable isolation and treatment facility in Sierra Leone.
Pelagia Reidy, Alie Wurie, Wayne Barnes, Emily Carr, David Kamau

J Infect· June 2017

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A conservative isoleucine to leucine mutation causes major rearrangements and cold sensitivity in KlenTaq1 DNA polymerase.
Eugene Y Wu, Amanda R Walsh, Emma C Materne, Emily P Hiltner, Bryan Zielinski, Wayne M Barnes

Biochemistry· January 2015

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Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq.
Zhian Zhang, Milko B Kermekchiev, Wayne M Barnes

J Mol Diagn· March 2010

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Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples.
Milko B Kermekchiev, Lyubka I Kirilova, Erika E Vail, Wayne M Barnes

Nucleic Acids Res· April 2009

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Streamlined gene assembly PCR.
Wayne M Barnes, Elaine R Frawley

CSH Protoc· March 2008

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Mass spectrometric and physiological validation of a sensitive, automated, direct immunoassay for serum estradiol using the Architect.
Patrick M Sluss, Frances J Hayes, Judith M Adams, Wayne Barnes, Gregg Williams

Clin Chim Acta· February 2008

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Long and accurate PCR.
Wayne M Barnes

CSH Protoc· June 2006

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Ribocloning: DNA cloning and gene construction using PCR primers terminated with a ribonucleotide.
Wayne M Barnes

CSH Protoc· June 2006

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The ETT2 gene cluster, encoding a second type III secretion system from Escherichia coli, is present in the majority of strains but has undergone widespread mutational attrition.
Chuan-Peng Ren, Roy R Chaudhuri, Amanda Fivian, Christopher M Bailey, Martin Antonio, Wayne M Barnes

J Bacteriol· June 2004

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Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR.
Milko B Kermekchiev, Anatoly Tzekov, Wayne M Barnes

Nucleic Acids Res· November 2003

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Universal PCR genotyping assay that achieves single copy sensitivity with any primer pair.
Jennifer L Stratman, Wayne M Barnes, Theodore C Simon

Transgenic Res· August 2003

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Mammalian microevolution: Rapid change in mouse mitochondrial DNA.
Oliver R W Pergams, Wayne M Barnes, Dennis Nyberg

Nature· May 2003

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Magnesium precipitate hot start method for PCR.
Wayne M Barnes, Katherine R Rowlyk

Mol Cell Probes· June 2002

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