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The discussions highlight key elements like lactose's advantages over IPTG and how glucose can prevent premature induction, demonstrating active interest in applying this research; phrases like 'useful food for thought' and 'probably play around' suggest engagement and exploration of practical techniques, while mentions of cost and media composition reflect an understanding of the study's broader implications.
Most discussions acknowledge the usefulness of the publication, especially regarding lactose and glucose effects on protein induction effectiveness.
Participants show high interest by exploring various implications, such as media composition and cost-effectiveness.
The conversations reflect moderate engagement, with comments on practical applications and methodological considerations.
Thoughts indicate recognition of the study's impact on improving protein production protocols, though not universally transformative.
@alchemytoday @samuelconnell @joachimgoedhart @TalleyJLambert @mpbruchez @erin_rod_phd @MillerChemBio @NathanShaner @HenriquesLab For low-fluorescent E. coli growth media (https://t.co/X64RVx9OgN) one can probably play around with some of the defined media for protein expression from the awesome Studier's paper (https://t.co/SAvRXrUCHH). Never tried this though so far. https://t.co/t3L9JtVxyt
@ATinyGreenCell @nelke_IGB Studier wrote a lot of information (for E coli) how different food sources affect the media pH (and can be used to counter each other) during protein production. Can be a useful food for thought in general. https://t.co/SAvRXrUCHH
@yogesh_jsharma @ATinyGreenCell Presence of glucose prevents the induction with lactose (import of lactose into E. coli), so include it or not according to your aims. https://t.co/SAvRXrUCHH
@revealplant @ATinyGreenCell No, reserve lactose for the auto-induction. https://t.co/SAvRXrUCHH
RT @galosgann: @ATinyGreenCell @nelke_IGB Studier wrote a lot of information (for E coli) how different food sources affect the media pH (a…
RT @galosgann: @GoldBio Usually lactose is better than IPTG in every way imaginable and is very cheap
Autoinduction offers an alternative to IPTG-based methods by using a glucose and lactose mix to control gene expression. Bacteria first consume glucose, then switch to lactose, turning on protein production efficiently…
Autoinduction offers an alternative to IPTG-based methods for gene expression under lac promoter control. It uses glucose and lactose media, allowing high-density cultures and reliable, high-yield protein production…
RT @galosgann: @ATinyGreenCell @nelke_IGB Studier wrote a lot of information (for E coli) how different food sources affect the media pH (a…
@ATinyGreenCell @nelke_IGB Studier wrote a lot of information (for E coli) how different food sources affect the media pH (and can be used to counter each other) during protein production. Can be a useful food for thought in general. https://t.co/SAvRXrUCHH
@yogesh_jsharma @ATinyGreenCell Presence of glucose prevents the induction with lactose (import of lactose into E. coli), so include it or not according to your aims. https://t.co/SAvRXrUCHH
RT @galosgann: @GoldBio Usually lactose is better than IPTG in every way imaginable and is very cheap
@revealplant @ATinyGreenCell No, reserve lactose for the auto-induction. https://t.co/SAvRXrUCHH
@alchemytoday @samuelconnell @joachimgoedhart @TalleyJLambert @mpbruchez @erin_rod_phd @MillerChemBio @NathanShaner @HenriquesLab For low-fluorescent E. coli growth media (https://t.co/X64RVx9OgN) one can probably play around with some of the defined media for protein expression from the awesome Studier's paper (https://t.co/SAvRXrUCHH). Never tried this though so far. https://t.co/t3L9JtVxyt
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