Science February 2013 PMID 23287718

Multiplex genome engineering using CRISPR/Cas systems.

Le Cong, F Ann Ran, David Cox, Shuailiang Lin, Robert Barretto, Naomi Habib, Patrick D Hsu, Xuebing Wu, Wenyan Jiang, Luciano A Marraffini, Feng Zhang

Mentions

235
Peak: 2 in Oct 2020
across 5 sources since Oct 2019

Extended reach

202,083
potential audience

Total likes

2,871

Video views

150,381

Mentions per month

Oct 2019 – Jul 2025

Where it's discussed

  • News153 articles
  • Blogs64 articles
  • X / Twitter10 posts
  • Facebook6 posts
  • YouTube2 videos

AI synopsis

What people are saying

The discussions express clear interest and recognition of the publication’s importance, often mentioning its role as a 'game-changer' in genome editing, and referencing examples like the use of Cas9 in human cells, highlighting both the scientific achievements and the potential for future medical applications. The tone varies from acknowledgment of its foundational status to curiosity about ongoing developments.

AgreementModerate agreement

Most discussions recognize the significance of the 2013 publication, with some highlighting its foundational role in CRISPR research.

InterestHigh interest

The topic garners high interest, especially considering the potential applications in medicine and genetics.

EngagementModerate engagement

Participants reference specific aspects of the research, such as the use of RNA guides and gene editing accuracy, indicating moderate depth of discussion.

ImpactModerate impact

Many see the publication as a pivotal moment that influences future research and therapeutics, though some note it is not a recent development.

Abstract in brief

  • CRISPR-Cas systems are powerful tools for editing genomes precisely, and they work by using RNA guides to direct the Cas9 enzyme to specific DNA sequences in human and mouse cells.
  • Researchers have shown that Cas9 can be modified to make single-strand cuts instead of double-strand breaks, which helps improve gene editing accuracy and reduces unwanted mutations.
  • Multiple guide RNAs can be combined in a single array, allowing the simultaneous editing of several genes at once, making CRISPR a flexible and versatile technology for genetic research.]

Top posts

Most liked on X and Bluesky
Ryan sikorski
@Ryansikorski10 · X

RNA-Guided Human Genome Engineering via Cas9 https://t.co/YyUjeDpgdA Multiplex genome engineering using CRISPR/Cas systems https://t.co/pAQ1VySRZW https://t.co/frGjZBBWIZ

Dec 11, 2024 · 6 likes · 1 share · 36,155 followers View post →
SEVAplasmids
@SEVAplasmids · X

So… Cas9 acts like a DNA surgeon in prokaryotes, but can CRISPR be used in mammalian cells?

Oct 1, 2021 · 2 likes · 5,297 followers View post →
GeneSoc
@GeneSoc1983 · X

Cong, L., Ran, F., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P., Wu, X., Jiang, W., Marraffini, L., & Zhang, F. (2013). Multiplex Genome Engineering using CRISPR/Cas Systems.

Nov 17, 2021 · 2 likes · 933 followers View post →
• Emily Therese •
@ActuallyMeEmily · X

@VAMNit Also: Cong, L., et al. (2013). Multiplex genome engineering using CRISPR/Cas systems. Super interesting reads. It's potential future use in hereditary diseases and other areas of gene selection is very interesting

Oct 10, 2020 · 1 like · 230 followers View post →
Isatou Sarr
@IsatouSarr4 · X

Multiplex genome engineering using CRISPR/Cas systems https://t.co/8Wc8U6cWNd

Sep 1, 2021 · 1 like · 46 followers View post →
TheFringe
@TheActaFist777 · X

RT @Ryansikorski10: RNA-Guided Human Genome Engineering via Cas9 https://t.co/YyUjeDpgdA Multiplex genome engineering using CRISPR/Cas sys…

Dec 11, 2024 · 1 share · 8,274 followers View post →

All mentions

Diya Borundiya
@DiyaBorundiya · X

Multiplex Genome Engineering Using CRISPR/Cas Systems: Dissecting the Paper https://t.co/yXsyeEv7xS

Jul 20, 2025 · 4 followers View post →
TheFringe
@TheActaFist777 · X

RT @Ryansikorski10: RNA-Guided Human Genome Engineering via Cas9 https://t.co/YyUjeDpgdA Multiplex genome engineering using CRISPR/Cas sys…

Dec 11, 2024 · 1 share · 8,274 followers View post →
Ryan sikorski
@Ryansikorski10 · X

RNA-Guided Human Genome Engineering via Cas9 https://t.co/YyUjeDpgdA Multiplex genome engineering using CRISPR/Cas systems https://t.co/pAQ1VySRZW https://t.co/frGjZBBWIZ

Dec 11, 2024 · 6 likes · 1 share · 36,155 followers View post →
Eduardo Suarez
@EduardoSuarez25 · X

@jblanca42 Parece que el 2013 fue súper "cine-genético". En enero se publicaba en Science "Multiplex Genome Engineering Using CRISPR/Cas Systems".

May 13, 2024 · 735 followers View post →
Gregor Huyskens
@Dr_Emyune · X

@IntuitMachine This is not new. 2013: Cong L, et al. Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013;339:819–823. [PMC free article] [PubMed] [Google Scholar]

Jul 3, 2023 · 25 followers View post →
GeneSoc
@GeneSoc1983 · X

Cong, L., Ran, F., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P., Wu, X., Jiang, W., Marraffini, L., & Zhang, F. (2013). Multiplex Genome Engineering using CRISPR/Cas Systems.

Nov 17, 2021 · 2 likes · 933 followers View post →
SEVAplasmids
@SEVAplasmids · X

So… Cas9 acts like a DNA surgeon in prokaryotes, but can CRISPR be used in mammalian cells?

Oct 1, 2021 · 2 likes · 5,297 followers View post →
Isatou Sarr
@IsatouSarr4 · X

Multiplex genome engineering using CRISPR/Cas systems https://t.co/8Wc8U6cWNd

Sep 1, 2021 · 1 like · 46 followers View post →
シュウ モトマツリコト
@BizarreZhouYZ · X

Multiplex Genome Engineering Using CRISPR/Cas Systems https://t.co/QbG8rZ3Mjq

Oct 26, 2020 View post →
• Emily Therese •
@ActuallyMeEmily · X

@VAMNit Also: Cong, L., et al. (2013). Multiplex genome engineering using CRISPR/Cas systems. Super interesting reads. It's potential future use in hereditary diseases and other areas of gene selection is very interesting

Oct 10, 2020 · 1 like · 230 followers View post →