An overview of technical considerations for Western blotting applications to physiological research.

J J Bass, D J Wilkinson, D Rankin, B E Phillips, N J Szewczyk, K Smith, P J Atherton

January 2017 Scand J Med Sci Sports

Synopsis of Social media discussions

The discussions emphasize the article's practical value, with phrases like 'important overview' and mentions of specific procedural steps such as electrophoresis and protein quantification, which reflect a respectful and engaged tone. The recognition of the publication as a leading and highly downloaded work highlights its significance and potential influence on research techniques.

A
Agreement
 Moderate agreement

Most posts acknowledge the importance and usefulness of the publication, especially highlighting its comprehensive overview and technical guidance.

I
Interest
 Moderate level of interest

The discussions show a moderate level of interest, mainly appreciating the article’s relevance to laboratory techniques and research practices.

E
Engagement
 Moderate level of engagement

Several posts demonstrate engagement by referencing specific methods like electrophoresis and Western blotting, and celebrating the achievement of the authors.

I
Impact
 Moderate level of impact

The publication appears to have a notable effect within the research community, as evidenced by its recognition as a top-cited and highly downloaded article.

Social Mentions

YouTube

2 Videos

Facebook

1 Posts

Twitter

9 Posts

Blogs

1 Articles

News

18 Articles

Metrics

Video Views

28,166

Total Likes

344

Extended Reach

34,318

Social Features

31

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Western Blot Analysis and Result Interpretation in Protein Research

Western Blot Analysis and Result Interpretation in Protein Research

The western blot is a widely used research technique that detects proteins using antibodies. This video covers analysis and interpretation of results, including immunoblotting methods, band analysis, quantification, and normalization, enhanced with laboratory footage and diagrams.

Demystifying Research McMaster

September 28, 2022

14,633 views

Western Blot Wet Transfer Method for Protein Analysis

Western Blot Wet Transfer Method for Protein Analysis

The western blot is a widely used research technique that detects proteins using antibodies. This video focuses on the wet transfer method for protein transfer onto membranes, listing materials, reagents, and procedural steps to ensure accurate and reliable results.

Demystifying Research McMaster

August 31, 2022

13,533 views

  • freebird
    @ifreecell (Twitter)

    An overview of technical considerations for Western blotting applications to physiological research. https://t.co/IqQo739J0p https://t.co/Y8HOf47JLH
    view full post

    November 25, 2020

  • Joan
    @resabut (Twitter)

    RT @Narcofa: Como resumen, podeis repasar en la foto todos los pasos llevados para la cuantificacion de proteinas. Electroforesis, western-…
    view full post

    May 2, 2019

    2

  • Fran Espejo
    @Narcofa (Twitter)

    Como resumen, podeis repasar en la foto todos los pasos llevados para la cuantificacion de proteinas. Electroforesis, western-blotting, etc. Sacada de: An overview of technical considerations for Western blotting applications to physiological research https://t.co/b8KS7wuyxV https://t.co/LXQXdwxDQQ
    view full post

    May 2, 2019

    2

  • Colleen Deane
    @csbd7 (Twitter)

    RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…
    view full post

    February 27, 2019

    5

  • Beth Phillips
    @MogwaiBeth (Twitter)

    RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…
    view full post

    February 27, 2019

    5

  • Salaheddin Sharif, MBChB, PhD, MS
    @salaymo (Twitter)

    "An overview of technical considerations for Western blotting applications to physiological research" https://t.co/pVvP1ybcXU
    view full post

    February 26, 2019

  • Jonny Quinlan
    @Jonnyquinlan (Twitter)

    RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…
    view full post

    February 26, 2019

    5

  • Ryan Marshall, PhD
    @RNMarshallPhD (Twitter)

    RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…
    view full post

    February 26, 2019

    5

  • Centre Of Metabolism, Ageing & Physiology (COMAP)
    @UNAD_Research (Twitter)

    Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applications to physiological research" being #TopCitedArticle (2nd most downloaded) in Scandinavian Journal of Medicine & Science In Sports. Well done all!
    view full post

    February 26, 2019

    14

    5

  • Vol. 52.5 State of Technology Report 2018 | PDF | Artificial Intelligence

    ... SMS messages ponent is a Coast Guard–regulated incidents but contains a ... 12702/2016-07- at-saudi-aramco/. AIAA/Utah State University. 07/32rrbk. 54 ...
    view full post

    December 21, 2025

    News

  • Recent Progress On Uric Acid Detection: A Review: Critical Reviews ...

    DOI: 10.1111/hepr.12734 ... Immobilized Uricase for Ultrasensitive Uric Acid Detection. [123] Krishnamoorthy, K.; Sudha, V.; Kumar, S. M. S.; Sens.
    view full post

    December 21, 2025

    News

  • R-SWITCH: Enhancing SAM Treatment in Ethiopia | PDF ...

    home surveillance of children with uncomplicated severe acute malnutrition. Matern Child Nutr. 2020;16(1):e12876.doi:10.1111/mcn.1287. Kebede, G. G., Dawed, ...
    view full post

    December 21, 2025

    News

  • Asteraceae Vernonieae Campo Rupestre | PDF | Leaf | Plant ...

    ); 12702 (XV.6.); 13024 (VI.6.); 13286 (III.2.). Guerreiro, S.M.C. et al. 32 ... S.M.S. et al. s.n. (UEC66129) (VI.13.). Verdi, M. et al. 6469 (XI.18 ...
    view full post

    December 21, 2025

    News

  • Henrique Cabral, Mario Lepage, Jérémy Lobry, Olivier Le Pape ...

    10.1111/bij.12635. Princeton University Press, Princeton, NJ. Inoue, J.G. ... 12702. dark. Front. Mar. Sci. 3 (March). https://doi.org/10.3389/FMARS ...
    view full post

    December 21, 2025

    News

  • You Mares | PDF | Conservation Biology | Ecological Resilience

    Cronin MA, McConnell BJ (2008) SMS seal: a new technique to mea- 21:1024–1032. ... org/10.1111/ddi.12702 Shoji A, Aris-Brosou S, Owen E et al (2016) Foraging ...
    view full post

    December 21, 2025

    News

  • Rice Protein Expression via Western Blot | PDF | Western Blot ...

    10.1111/sms.12702 Gorr TA, Vogel J (2015) Western blotting. Chatterjee M, Gupta S, Bhar A, Das S (2012) revisited: Critical perusal of. Optimization of an ...
    view full post

    December 21, 2025

    News

  • Scandinavian Med Sci Sports - 2016 - Bass - An Overview of ...

    or membrane bound? for proteins ~75 kDa, MES for proteins <36 kDa)?. 5 16000838, 2017, 1, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/sms.12702, ...
    view full post

    December 21, 2025

    News

  • From_single_cell_maps_to_diagnostics_ena | PDF | Proteomics ...

    10.1111/sms.12702 s.org/licenses/by/4.0/). 12. Meier-Soelch J, Mayr-Buro C, Juli J, Leib L, Linne U, Dreute J, et al. Monitoring the levels of cellular NF ...
    view full post

    December 21, 2025

    News

  • Neurología | PDF | Cerebellum | Dendrite

    2017;27:4—25, 6. Akhlaghpasand M, Tizro M, Raoofi A, Meymand AZ, http://dx.doi.org/10.1111/sms.12702.
    view full post

    December 21, 2025

    News

  • Exploring Group 1 Metabotropic Glutamate Receptors in The Patholo

    https://doi.org/10.1111/sms.12702. Bauer, D., Haroutunian, V., Meador-Woodruff, J.H., McCullumsmith, R.E., 2010. Abnormal glycosylation of EAAT1 and EAAT2 in ...
    view full post

    December 21, 2025

    News

  • Western Blotting Guide - Part 2, Protein Separation by SDS-PAGE ...

    10.1111/sms.12702 7. Lewis M. (2018). A guide to selecting control and blocking reagents. Jackson ImmunoResearch. Laboratories Inc. https://www ...
    view full post

    December 21, 2025

    News

  • Methods | PDF | Southern Blot | Microscope

    (so you can see it) that will stick only to the first antibody (not to the target protein). Image: http://onlinelibrary.wiley.com/doi/10.1111/sms.12702/full. 49
    view full post

    December 21, 2025

    News

  • Regulation and Dialogue on Social Media: Exploring Democratic ...

    ... (SMS@MIT): "Social media has become a dominant force for information ... doi: 10.1111/j.1468-2885.2006.00280.x Kate Kenski and Talia ...
    view full post

    August 23, 2025

    Facebook

  • Western Blot Procedures, Analysis and Purpose | Technology ...

    doi:10.1111/sms.12702. 5. Welinder C, Ekblad L. Coomassie staining as loading control in western blot analysis. J Proteome Res. 2011;10(3): ...
    view full post

    December 18, 2023

    News

  • WB实验步骤、分析和目的_搜狐网

    doi:10.1111/sms.127025. Welinder C, Ekblad L.Coomassie staining as loading control in western blot analysis. J Proteome Res.2011;10(3):1416 ...
    view full post

    February 6, 2023

    News

  • Comorbid Conditions of Attention deficit / hyperactivity disorder ...

    Editor: Blackwell Publishing Inc. DOI: 10.1111/sms.13356. Genetic and ... Editor: Blackwell Publishing Inc. DOI: 10.1111/jcpp.12702.
    view full post

    August 24, 2022

    News

  • Comorbid Conditions of Attention deficit / hyperactivity disorder ...

    Publisher: Blackwell Publishing Inc. DOI: 10.1111/sms.13356. Genetic ... Publisher: Blackwell Publishing Inc. DOI: 10.1111/jcpp.12702.
    view full post

    August 24, 2022

    News

  • Crisis Support Lines: What Are We Missing? | Psychology Today ...

    https://doi.org/10.1111/sltb.12702. Kalafat, J., Gould, M. S. ... Evaluation of Outcomes for Help Seekers Accessing a Pilot SMS-Based Crisis ...
    view full post

    June 16, 2022

    Blogs

  • MONASH SCHOOL OF PUBLIC HEALTH AND PREVENTIVE ...

    DOI: 10.1111/sms.12698. Firth J, Stubbs B, Sarris J, Rosenbaum S ... DOI: 10.1111/jep.12702. Hay SI, Abajobir AA, Abate KH, et al ...
    view full post

    July 17, 2018

    News

Abstract Synopsis

  • Western immunoblotting (WB) techniques are widely used in physiology research, especially for studying skeletal muscle responses to exercise, and are now a standard method for understanding regulatory mechanisms.
  • The quality and reliability of WB data can vary due to differences in laboratory techniques and a lack of understanding of critical procedural steps, potentially leading to misleading conclusions.
  • This review offers a detailed guide on WB procedures, from sample collection to data analysis, helping researchers improve their skills, ensure reproducibility, and troubleshoot issues for more accurate results.]