Scand J Med Sci Sports January 2017 PMID 27263489

An overview of technical considerations for Western blotting applications to physiological research.

J J Bass, D J Wilkinson, D Rankin, B E Phillips, N J Szewczyk, K Smith, P J Atherton

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Mentions

31
Peak: 6 in Feb 2019
across 5 sources since Jul 2018

Extended reach

34,318
potential audience

Total likes

344

Video views

28,166

Mentions per month

Jul 2018 – Aug 2025

Where it's discussed

  • News18 articles
  • X / Twitter9 posts
  • YouTube2 videos
  • Facebook1 posts
  • Blogs1 articles

AI synopsis

What people are saying

The discussions emphasize the article's practical value, with phrases like 'important overview' and mentions of specific procedural steps such as electrophoresis and protein quantification, which reflect a respectful and engaged tone. The recognition of the publication as a leading and highly downloaded work highlights its significance and potential influence on research techniques.

AgreementModerate agreement

Most posts acknowledge the importance and usefulness of the publication, especially highlighting its comprehensive overview and technical guidance.

InterestModerate interest

The discussions show a moderate level of interest, mainly appreciating the article’s relevance to laboratory techniques and research practices.

EngagementModerate engagement

Several posts demonstrate engagement by referencing specific methods like electrophoresis and Western blotting, and celebrating the achievement of the authors.

ImpactModerate impact

The publication appears to have a notable effect within the research community, as evidenced by its recognition as a top-cited and highly downloaded article.

Abstract in brief

  • Western immunoblotting (WB) techniques are widely used in physiology research, especially for studying skeletal muscle responses to exercise, and are now a standard method for understanding regulatory mechanisms.
  • The quality and reliability of WB data can vary due to differences in laboratory techniques and a lack of understanding of critical procedural steps, potentially leading to misleading conclusions.
  • This review offers a detailed guide on WB procedures, from sample collection to data analysis, helping researchers improve their skills, ensure reproducibility, and troubleshoot issues for more accurate results.]

Top posts

Most liked on X and Bluesky
Centre Of Metabolism, Ageing & Physiology (COMAP)
@UNAD_Research · X

Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applications to physiological research" being #TopCitedArticle (2nd most downloaded) in Scandinavian Journal of Medicine & Science In Sports. Well done all!

Feb 26, 2019 · 14 likes · 5 shares · 584 followers View post →
Colleen Deane
@csbd7 · X

RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…

Feb 27, 2019 · 5 shares · 1,623 followers View post →
Ryan Marshall, PhD
@RNMarshallPhD · X

RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…

Feb 26, 2019 · 5 shares · 1,234 followers View post →
Beth Phillips
@MogwaiBeth · X

RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…

Feb 27, 2019 · 5 shares · 820 followers View post →
freebird
@ifreecell · X

An overview of technical considerations for Western blotting applications to physiological research. https://t.co/IqQo739J0p https://t.co/Y8HOf47JLH

Nov 25, 2020 · 413 followers View post →
Jonny Quinlan
@Jonnyquinlan · X

RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…

Feb 26, 2019 · 5 shares · 391 followers View post →

All mentions

freebird
@ifreecell · X

An overview of technical considerations for Western blotting applications to physiological research. https://t.co/IqQo739J0p https://t.co/Y8HOf47JLH

Nov 25, 2020 · 413 followers View post →
Joan
@resabut · X

RT @Narcofa: Como resumen, podeis repasar en la foto todos los pasos llevados para la cuantificacion de proteinas. Electroforesis, western-…

May 2, 2019 · 2 shares · 285 followers View post →
Fran Espejo
@Narcofa · X

Como resumen, podeis repasar en la foto todos los pasos llevados para la cuantificacion de proteinas. Electroforesis, western-blotting, etc. Sacada de: An overview of technical considerations for Western blotting applications to physiological research https://t.co/b8KS7wuyxV https://t.co/LXQXdwxDQQ

May 2, 2019 · 2 shares · 352 followers View post →
Colleen Deane
@csbd7 · X

RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…

Feb 27, 2019 · 5 shares · 1,623 followers View post →
Beth Phillips
@MogwaiBeth · X

RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…

Feb 27, 2019 · 5 shares · 820 followers View post →
Salaheddin Sharif, MBChB, PhD, MS
@salaymo · X

"An overview of technical considerations for Western blotting applications to physiological research" https://t.co/pVvP1ybcXU

Feb 26, 2019 · 193 followers View post →
Jonny Quinlan
@Jonnyquinlan · X

RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…

Feb 26, 2019 · 5 shares · 391 followers View post →
Ryan Marshall, PhD
@RNMarshallPhD · X

RT @UNAD_Research: Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applic…

Feb 26, 2019 · 5 shares · 1,234 followers View post →
Centre Of Metabolism, Ageing & Physiology (COMAP)
@UNAD_Research · X

Great achievement for @JosephJBass and our lab with "An overview of technical considerations for Western blotting applications to physiological research" being #TopCitedArticle (2nd most downloaded) in Scandinavian Journal of Medicine & Science In Sports. Well done all!

Feb 26, 2019 · 14 likes · 5 shares · 584 followers View post →
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