Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads.

Yohei Sasagawa, Hiroki Danno, Hitomi Takada, Masashi Ebisawa, Kaori Tanaka, Tetsutaro Hayashi, Akira Kurisaki, Itoshi Nikaido

March 2018 Genome Biol

Synopsis of Social media discussions

Discussions reflect a positive consensus on Quartz-Seq2, with users emphasizing its sensitivity and efficiency, such as mentions of its ability to analyze thousands of transcriptomes with fewer reads, and terms like 'high-throughput' and 'effective use of limited sequence reads' underline its impactful innovations.

A
Agreement
 Moderate agreement

Most discussions recognize Quartz-Seq2 as a highly sensitive and effective method, supporting the publication's claims.

I
Interest
 High level of interest

Many posts show strong curiosity about the technique, mentioning its high-throughput capability and potential applications.

E
Engagement
 High engagement

Several social discussions delve into specific features like read length effects and practical use cases, indicating in-depth engagement.

I
Impact
 High level of impact

Posts highlight the method's transformative potential in single-cell analysis and its utility with limited reads, suggesting significant impact.

Social Mentions

YouTube

2 Videos

Bluesky

1 Posts

Twitter

35 Posts

Blogs

2 Articles

News

2 Articles

Metrics

Video Views

2,700

Total Likes

62

Extended Reach

161,402

Social Features

42

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Quartz-Seq2: High-Throughput Single-Cell RNA Sequencing Method

Quartz-Seq2: High-Throughput Single-Cell RNA Sequencing Method

QuartzSeq2 is a new high-throughput single-cell RNA sequencing method designed to improve gene expression measurement accuracy using limited sequence reads. It efficiently converts initial reads into UMI counts, detecting more genes than previous approaches.

ecPDCNRA-work

December 26, 2017

1,775 views

Quartz-Seq2: Improved High-Throughput Single-Cell RNA Sequencing Technique

Quartz-Seq2: Improved High-Throughput Single-Cell RNA Sequencing Technique

QuartzSeq2 is a new high-throughput single-cell RNA sequencing method designed to improve the accuracy of gene expression measurement, especially when using limited sequence reads. It enhances gene detection efficiency, enabling detailed transcriptome analysis with fewer reads.

ecPDCNRA-work

May 8, 2018

926 views

  • Itoshi NIKAIDO
    @dritoshien (Twitter)

    We measured single-cell transcriptome data from the stomach of adult mice using Quartz-Seq2, a highly sensitive single-cell RNA-seq method. (2/n) https://t.co/JbojpDsahZ
    view full post

    July 3, 2023

  • Y-SASA
    @ecPDCNRA (Twitter)

    高田先生、栗崎先生は、我々が開発したQuartz-Seq2の技術開発論文 https://t.co/KODk7R58oi に参加していただいていて、今回はその技術を応用していただいたという形になりとても嬉しいです。 先生方のラボのHPはこちら。https://t.co/fbu4RN3pO8　[8/n]
    view full post

    June 29, 2023

    2

  • Y-SASA
    @ecPDCNRA (Twitter)

    @KKo1wa1 一応single-cell qPCRという手法があります。 https://t.co/KODk7QMZaa このFig6fとか。FACSつかうからハードル高めかもです。
    view full post

    May 31, 2022

    2

  • Itoshi NIKAIDO
    @dritoshien (Twitter)

    Quartz-Seq2 is a highly sensitive single-cell RNA-seq method. https://t.co/NPymcrlona https://t.co/JbojpDsahZ
    view full post

    February 15, 2022

  • Itoshi NIKAIDO
    @dritoshien (Twitter)

    RT @ecPDCNRA: The reaction solution can be recovered from each well of the 384PCR plate at once by spin-down. We have previously reported i…
    view full post

    July 12, 2021

    1

  • Y-SASA
    @ecPDCNRA (Twitter)

    The reaction solution can be recovered from each well of the 384PCR plate at once by spin-down. We have previously reported in Q2 paper at 2018 If you email the contact information in the link, they will make a metal frame for you. https://t.co/PvpFwENqeg https://t.co/6M6cxpT4YM
    view full post

    July 12, 2021

    1

    1

  • Y-SASA
    @ecPDCNRA (Twitter)

    @Tamura_Lab とても共感しました。 自分たちは、パナソニックのヘッドマウント型で撮ってます。↓ https://t.co/zppETjGSeP まだ試せていませんが、OBSBOT Tailだと ジェスチャーでの撮影開始と自動追尾ができるので、マクロでなければ、自分一人で実験を取れるかもしれません。 https://t.co/lZHC4t0RmC
    view full post

    June 7, 2020

    1

  • Joshua Ho
    @joshuawkho (Twitter)

    RT @LGMartelotto: A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads…
    view full post

    April 18, 2020

    7

  • Carlos Canchaya
    @aukicha (Twitter)

    RT @LGMartelotto: A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads…
    view full post

    April 17, 2020

    7

  • Ming "Tommy" Tang
    @tangming2005 (Twitter)

    RT @LGMartelotto: A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads…
    view full post

    April 17, 2020

    7

  • Single Cell Media
    @SingleCellMedia (Twitter)

    RT @LGMartelotto: A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads…
    view full post

    April 17, 2020

    7

  • Luciano Martelotto
    @LGMartelotto (Twitter)

    A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads | Genome Biology | Full Text https://t.co/0oak65TCRS
    view full post

    April 17, 2020

    22

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  • realAkiraWatanabe
    @sfwaesr (Twitter)

    RT @ecPDCNRA: Metal frameの情報をupdateしてますが、周知できていなかったことに気づきました。改めてアナウンス。https://t.co/zTeY2aAFgL 薬研社の上條さんに連絡ください。96, 384 well plateから、遠心力により、一…
    view full post

    August 31, 2019

    1

  • Y-SASA
    @ecPDCNRA (Twitter)

    Metal frameの情報をupdateしてますが、周知できていなかったことに気づきました。改めてアナウンス。https://t.co/zTeY2aAFgL 薬研社の上條さんに連絡ください。96, 384 well plateから、遠心力により、一気に中身の溶液を一つに集めるためのものです。#SCGJ2019 https://t.co/gnZlpkR5PG
    view full post

    August 31, 2019

    1

    1

  • Dr. Neil Ashley
    @NotWIMM (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 21, 2019

    13

  • RIKEN BDR
    @BDR_RIKEN (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • Haruka Ozaki (尾崎遼)
    @yuifu (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • Tom Kelly ケリー・トム
    @tomkXY (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • steven hoffman
    @hoffman_steven (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • know thyself（子豚のオリバー)
    @thyself_know (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • Ming "Tommy" Tang
    @tangming2005 (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • Holger Heyn
    @hoheyn (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • Elisabetta Mereu
    @eli_mereu (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • キケ•ケロナンデス
    @suimye (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • もじゃもじゃのほう@求職中
    @antiplastics (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • ゲノムのほうの愛ちゃん
    @dritoshi (Twitter)

    RT @dritoshien: If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ http…
    view full post

    May 20, 2019

    13

  • Itoshi NIKAIDO
    @dritoshien (Twitter)

    If you are interested in Quartz-Seq2, please visit the following URLs: https://t.co/KRy0Ktq7vx https://t.co/JbojpDJdjZ https://t.co/aaloIAR5gg https://t.co/knbOkTF0KL
    view full post

    May 20, 2019

    18

    13

  • サクセスリバーコーテシーinエイトプリンス
    @rei_nari (Twitter)

    RT @dritoshi: なぜ検出遺伝子数に優れるのかは以下をどうぞ。https://t.co/8C6ISmDy1m https://t.co/n8hrE65oqa https://t.co/8UUG2vhFXj
    view full post

    May 16, 2019

    6

  • Kemtaro
    @oxt23 (Twitter)

    RT @dritoshi: なぜ検出遺伝子数に優れるのかは以下をどうぞ。https://t.co/8C6ISmDy1m https://t.co/n8hrE65oqa https://t.co/8UUG2vhFXj
    view full post

    May 14, 2019

    6

  • もり
    @mad_molix (Twitter)

    RT @dritoshi: なぜ検出遺伝子数に優れるのかは以下をどうぞ。https://t.co/8C6ISmDy1m https://t.co/n8hrE65oqa https://t.co/8UUG2vhFXj
    view full post

    May 14, 2019

    6

  • wvk1
    @wvk110 (Twitter)

    RT @dritoshi: なぜ検出遺伝子数に優れるのかは以下をどうぞ。https://t.co/8C6ISmDy1m https://t.co/n8hrE65oqa https://t.co/8UUG2vhFXj
    view full post

    May 14, 2019

    6

  • TSK
    @TSK010 (Twitter)

    RT @dritoshi: なぜ検出遺伝子数に優れるのかは以下をどうぞ。https://t.co/8C6ISmDy1m https://t.co/n8hrE65oqa https://t.co/8UUG2vhFXj
    view full post

    May 14, 2019

    6

  • ゲノムのほうの愛ちゃん
    @dritoshi (Twitter)

    なぜ検出遺伝子数に優れるのかは以下をどうぞ。https://t.co/8C6ISmDy1m https://t.co/n8hrE65oqa https://t.co/8UUG2vhFXj
    view full post

    May 14, 2019

    11

    6

  • Tai
    @tai_research (Twitter)

    RT @ecPDCNRA: Effect of read2 length (scRNA-seq). https://t.co/LmIbW95aqd 聞かれる頻度が高いので備忘録。Q2での経験だと、Unique mapping率とUMIcountに影響。gene countには…
    view full post

    May 11, 2019

    1

  • Y-SASA
    @ecPDCNRA (Twitter)

    Effect of read2 length (scRNA-seq). https://t.co/LmIbW95aqd 聞かれる頻度が高いので備忘録。Q2での経験だと、Unique mapping率とUMIcountに影響。gene countには、影響が少ない。特定の遺伝子は全く検出されないことがある。他の方法でも同傾向と推測される。Q2ではコスパが一番良い62ntを採用。 https://t.co/qKUzODqRl6
    view full post

    May 10, 2019

    3

    1

  • Tara Moriarty
    @moriartylab.bsky.social (Bluesky)

    Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1407-3
    view full post

    March 12, 2018

Abstract Synopsis

  • QuartzSeq2 is a new high-throughput single-cell RNA sequencing method designed to improve the accuracy of gene expression measurement, especially when using limited sequence reads.
  • The method enhances the conversion of initial sequence reads into UMI counts at a rate of 30-50%, allowing for the detection of more genes compared to previous approaches.
  • To showcase its effectiveness, QuartzSeq2 was used to analyze around 10,000 transcriptomes from embryonic stem cells and stromal vascular tissue, demonstrating its ability to generate detailed gene expression data with fewer reads.]