Genome Biol March 2018 PMID 29523163

Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads.

Yohei Sasagawa, Hiroki Danno, Hitomi Takada, Masashi Ebisawa, Kaori Tanaka, Tetsutaro Hayashi, Akira Kurisaki, Itoshi Nikaido

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Mentions

Peak: 21 in May 2019

across 5 sources since Dec 2017

Extended reach

161,402

potential audience

Total likes

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2,700

Mentions per month

Dec 2017 – Jul 2023

Where it's discussed

X / Twitter35 posts
YouTube2 videos
News2 articles
Blogs2 articles
Bluesky1 posts

AI synopsis

What people are saying

Discussions reflect a positive consensus on Quartz-Seq2, with users emphasizing its sensitivity and efficiency, such as mentions of its ability to analyze thousands of transcriptomes with fewer reads, and terms like 'high-throughput' and 'effective use of limited sequence reads' underline its impactful innovations.

AgreementModerate agreement

Most discussions recognize Quartz-Seq2 as a highly sensitive and effective method, supporting the publication's claims.

InterestHigh interest

Many posts show strong curiosity about the technique, mentioning its high-throughput capability and potential applications.

EngagementHigh engagement

Several social discussions delve into specific features like read length effects and practical use cases, indicating in-depth engagement.

ImpactHigh impact

Posts highlight the method's transformative potential in single-cell analysis and its utility with limited reads, suggesting significant impact.

Abstract in brief

QuartzSeq2 is a new high-throughput single-cell RNA sequencing method designed to improve the accuracy of gene expression measurement, especially when using limited sequence reads.
The method enhances the conversion of initial sequence reads into UMI counts at a rate of 30-50%, allowing for the detection of more genes compared to previous approaches.
To showcase its effectiveness, QuartzSeq2 was used to analyze around 10,000 transcriptomes from embryonic stem cells and stromal vascular tissue, demonstrating its ability to generate detailed gene expression data with fewer reads.]

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All mentions

Quartz-Seq2: High-Throughput Single-Cell RNA Sequencing Method

QuartzSeq2 is a new high-throughput single-cell RNA sequencing method designed to improve gene expression measurement accuracy using limited sequence reads. It efficiently converts initial reads into UMI counts…

ecPDCNRA-work · Dec 26, 2017 1,775 views

Quartz-Seq2: Improved High-Throughput Single-Cell RNA Sequencing Technique

QuartzSeq2 is a new high-throughput single-cell RNA sequencing method designed to improve the accuracy of gene expression measurement, especially when using limited sequence reads. It enhances gene detection efficiency…

ecPDCNRA-work · May 8, 2018 926 views

Itoshi NIKAIDO

@dritoshien · X

We measured single-cell transcriptome data from the stomach of adult mice using Quartz-Seq2, a highly sensitive single-cell RNA-seq method. (2/n) https://t.co/JbojpDsahZ

Jul 3, 2023 · 554 followers View post →

Y-SASA

@ecPDCNRA · X

Jun 29, 2023 · 2 likes · 637 followers View post →

Y-SASA

@ecPDCNRA · X

@KKo1wa1 一応single-cell qPCRという手法があります。 https://t.co/KODk7QMZaa このFig6fとか。FACSつかうからハードル高めかもです。

May 31, 2022 · 2 likes · 637 followers View post →

Itoshi NIKAIDO

@dritoshien · X

Quartz-Seq2 is a highly sensitive single-cell RNA-seq method. https://t.co/NPymcrlona https://t.co/JbojpDsahZ

Feb 15, 2022 · 554 followers View post →

Itoshi NIKAIDO

@dritoshien · X

RT @ecPDCNRA: The reaction solution can be recovered from each well of the 384PCR plate at once by spin-down. We have previously reported i…

Jul 12, 2021 · 1 share · 553 followers View post →

Y-SASA

@ecPDCNRA · X

The reaction solution can be recovered from each well of the 384PCR plate at once by spin-down. We have previously reported in Q2 paper at 2018 If you email the contact information in the link, they will make a metal frame for you. https://t.co/PvpFwENqeg https://t.co/6M6cxpT4YM

Jul 12, 2021 · 1 like · 1 share · 628 followers View post →

Y-SASA

@ecPDCNRA · X

@Tamura_Lab とても共感しました。自分たちは、パナソニックのヘッドマウント型で撮ってます。↓ https://t.co/zppETjGSeP まだ試せていませんが、OBSBOT Tailだとジェスチャーでの撮影開始と自動追尾ができるので、マクロでなければ、自分一人で実験を取れるかもしれません。 https://t.co/lZHC4t0RmC

Jun 7, 2020 · 1 like · 628 followers View post →

Joshua Ho

@joshuawkho · X

RT @LGMartelotto: A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads…

Apr 18, 2020 · 7 shares · 1,042 followers View post →

Carlos Canchaya

@aukicha · X

RT @LGMartelotto: A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads…

Apr 17, 2020 · 7 shares · 209 followers View post →

Ming "Tommy" Tang

@tangming2005 · X

RT @LGMartelotto: A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads…

Apr 17, 2020 · 7 shares · 41,838 followers View post →

Single Cell Media

@SingleCellMedia · X

RT @LGMartelotto: A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads…

Apr 17, 2020 · 7 shares · 3,825 followers View post →

Luciano Martelotto

@LGMartelotto · X

A revisit: Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads | Genome Biology | Full Text https://t.co/0oak65TCRS

Apr 17, 2020 · 22 likes · 7 shares · 10,207 followers View post →

realAkiraWatanabe

@sfwaesr · X

RT @ecPDCNRA: Metal frameの情報をupdateしてますが、周知できていなかったことに気づきました。改めてアナウンス。https://t.co/zTeY2aAFgL 薬研社の上條さんに連絡ください。96, 384 well plateから、遠心力により、一…

Aug 31, 2019 · 1 share · 785 followers View post →

Y-SASA

@ecPDCNRA · X

Metal frameの情報をupdateしてますが、周知できていなかったことに気づきました。改めてアナウンス。https://t.co/zTeY2aAFgL 薬研社の上條さんに連絡ください。96, 384 well plateから、遠心力により、一気に中身の溶液を一つに集めるためのものです。#SCGJ2019 https://t.co/gnZlpkR5PG

Aug 31, 2019 · 1 like · 1 share · 628 followers View post →