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Examples show that discussions emphasize the technological innovations, such as the SCoPE-MS and SCoPE2 protocols, using enthusiastic terms like 'beautiful paper' and 'game-changing.' The tone and choice of words highlight the community's recognition of the article’s importance and engagement with its technical details, indicating a positive outlook on its scientific impact.
Most discussions acknowledge the significance and technical advancements presented in the publication, reflecting general agreement with its contributions.
There is high interest due to the relevance of single-cell proteomics and recent technological improvements discussed in the social deliberations.
Participants reference specific methods, such as the SCoPE-MS protocol and technological challenges, indicating active engagement and familiarity with the content.
Users recognize the publication's potential to influence future research and technological development in the field, affirming its moderate to high impact.
This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody else had done it earlier. https://t.co/cAKLET86Wd https://t.co/zfxDwh5ixc
When we started this project - about 10 years ago - we had limited access to old equipment. Better equipment would have been helpful, but using older equipment changed the perception about the feasibility & accessibility of single-cell proteomics. https://t.co/cAKLET86Wd https://t.co/1huPIRJmKP
The 2023 Google Metrics are out, and several papers from @slavovLab are listed within the h5-indices of their journals, including: https://t.co/cAKLET86Wd at Genome Biology https://t.co/8Agmu0HjiO at Genome Biology https://t.co/peylTx0OXy at J. Proteome Res. Genome Biology https://t.co/dHS8WGCy7o
… The SCoPE2 protocol allows analyzing > 200 single cells / day. This throughput is higher than for SCoPE-MS https://t.co/cAKLET86Wd, and I look forward to increasing it further as previously discussed: https://t.co/peylTx0OXy
2/ ... I felt very confident in the core ideas & conclusions. Thus, I felt it was just a matter of time until others reach that confidence. The reviews at @GenomeBiology were much more thoughtful & constructive. https://t.co/cAKLET86Wd
The 2022 Google Metrics are out https://t.co/gALibbmuNr Several papers from @slavovLab are listed within the h5-indices of their journals, including: https://t.co/peylTx0OXy at @JProteomeRes https://t.co/EXrnHYM17V at @PLOSCompBiol https://t.co/cAKLET86Wd at @GenomeBiology
In this presentation, Nikolai Slavov discusses miniaturizing and automating sample preparation for single-cell proteomics. Emphasizing key challenges and solutions, he presents single-cell protein measurements and…
This presentation by Nikolai Slavov introduces SCoPE2, a novel technology that enhances single-cell proteomics analysis. It focuses on quantifying over 2000 proteins in individual monocytes and macrophages, highlighting…
Presentation by Ed Emmott discussing the SCoPE2 pipeline for processing single-cell samples, emphasizing automation and high throughput, essential for accurate proteomics analysis in small samples.
Nikolai Slavov discusses progress and standards in single-cell proteomics, highlighting how mass spectrometry can revolutionize medical research by enabling precise measurement of proteins in individual cells. These…
Protein analysis by shotgun and bottom-up proteomics. The video explores advanced methods such as MALDI Imaging Mass Spectrometry and insights from recent research in proteomics and bioinformatics.
Gray Huffman discusses experimental and computational methods for optimizing single-cell mass spec analysis, focusing on setting optimal parameters for improved sensitivity and accuracy in proteomics research.
RT @slavov_n: When we started this project - about 10 years ago - we had limited access to old equipment. Better equipment would have be…
RT @slavov_n: When we started this project - about 10 years ago - we had limited access to old equipment. Better equipment would have be…
RT @slavov_n: When we started this project - about 10 years ago - we had limited access to old equipment. Better equipment would have be…
RT @slavov_n: When we started this project - about 10 years ago - we had limited access to old equipment. Better equipment would have be…
When we started this project - about 10 years ago - we had limited access to old equipment. Better equipment would have been helpful, but using older equipment changed the perception about the feasibility & accessibility of single-cell proteomics. https://t.co/cAKLET86Wd https://t.co/1huPIRJmKP
RT @slavov_n: 2/ ... I felt very confident in the core ideas & conclusions. Thus, I felt it was just a matter of time until others…
2/ ... I felt very confident in the core ideas & conclusions. Thus, I felt it was just a matter of time until others reach that confidence. The reviews at @GenomeBiology were much more thoughtful & constructive. https://t.co/cAKLET86Wd
RT @slavov_n: @DsSvetlov I agree. I started doing mass spec proteomics using facilities, including for sensitive and demanding projects lik…
@DsSvetlov I agree. I started doing mass spec proteomics using facilities, including for sensitive and demanding projects like SCoPE-MS https://t.co/cAKLET86Wd The cost of MS instruments was a major bottleneck for us. Relieving it with a discounted QE was empowering.
RT @slavov_n: The 2023 Google Metrics are out, and several papers from @slavovLab are listed within the h5-indices of their journals, inclu…
RT @slavov_n: The 2023 Google Metrics are out, and several papers from @slavovLab are listed within the h5-indices of their journals, inclu…
RT @slavov_n: The 2023 Google Metrics are out, and several papers from @slavovLab are listed within the h5-indices of their journals, inclu…
RT @slavov_n: The 2023 Google Metrics are out, and several papers from @slavovLab are listed within the h5-indices of their journals, inclu…
The 2023 Google Metrics are out, and several papers from @slavovLab are listed within the h5-indices of their journals, including: https://t.co/cAKLET86Wd at Genome Biology https://t.co/8Agmu0HjiO at Genome Biology https://t.co/peylTx0OXy at J. Proteome Res. Genome Biology https://t.co/dHS8WGCy7o
According to rule 34 of single cell, yes, it IS! https://t.co/ue57WhSTGS https://t.co/jiz5tfrU1E
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
RT @slavov_n: This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody e…
This paper was rather late. The essential equipment & tools that we used were available circa 2010. I wish we or somebody else had done it earlier. https://t.co/cAKLET86Wd https://t.co/zfxDwh5ixc
RT @slavov_n: The 2022 Google Metrics are out https://t.co/gALibbmuNr Several papers from @slavovLab are listed within the h5-indices of t…
The 2022 Google Metrics are out https://t.co/gALibbmuNr Several papers from @slavovLab are listed within the h5-indices of their journals, including: https://t.co/peylTx0OXy at @JProteomeRes https://t.co/EXrnHYM17V at @PLOSCompBiol https://t.co/cAKLET86Wd at @GenomeBiology
Pada 2018, Nicolai Slavov dan timnya menjelaskan protokol lisis sel untuk analisis MS. Metodenya dinamakan SCoPE-MS (single-cell proteomics by mass spectrometry). https://t.co/QwzdOx9GR8
SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation https://t.co/iTMeQKBapW
… The SCoPE2 protocol allows analyzing > 200 single cells / day. This throughput is higher than for SCoPE-MS https://t.co/cAKLET86Wd, and I look forward to increasing it further as previously discussed: https://t.co/peylTx0OXy
@RuvinskyIlya @slavovLab The peer review took some time, and it was easier to bear given that the results, data and code where publicly available & part of the discussions. While the peer reviewed version https://t.co/cAKLET86Wd is cited more frequently, I felt the preprint was the real publication.
@Afrobodies @sciencemagazine Mass-spec instruments can detect single ions as demonstrated in this beautiful paper by @NLKProteomics et al https://t.co/k5AFU4W3or We quantified proteins present at 50k copies / cell while optimizing throughout, so that is not a limit of sensitivity https://t.co/cAKLET86Wd
@AlbertVilella While Cytof is there no other Proteomics methods are present in the list. NanoPOTS, https://t.co/NlmpLxvnt2 BASIL https://t.co/ZJhWFYHhz1 DIA-sc-proteomics https://t.co/NlmpLxvnt2 And scope https://t.co/rJEvoGR1dZ should be added.
RT @Smith_Chem_Wisc: Hey @slavovLab this is a pretty great paper. SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome…
RT @Smith_Chem_Wisc: Hey @slavovLab this is a pretty great paper. SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome…
RT @Smith_Chem_Wisc: Hey @slavovLab this is a pretty great paper. SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome…
Hey @slavovLab this is a pretty great paper. SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation https://t.co/tBpRbzXO64
RT @GrayHuffman: Low-input samples are more substantially impacted by the presence of co-eluting non-peptidic ions, and having a means to i…
Low-input samples are more substantially impacted by the presence of co-eluting non-peptidic ions, and having a means to identify the impact of these contaminant ions can be quite helpful when evaluating/comparing SCoPE-style (https://t.co/CyYYXTXjCh) experiments.
When adopting or optimizing low-input sample methods (such as SCoPE-MS: https://t.co/CyYYXTXjCh), increasing the number and purity of sampled ions is particularly important. DO-MS allowed the Slavov Lab to better sample the elution peak apex by optimizing our duty cycle. https://t.co/iTq2CKrSj6
@slavovLab @afranks53 @NUBioE1 With our single cell SCoPE-MS data (https://t.co/OFIcXxMVzb), low-abundance peptides with noisier spectra were frequently receiving low identification confidence - making them unusable for downstream analyses or requiring us to impute data. 3/