Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycoprotein afamin.

Andreas Naschberger, Pauline Juyoux, Jill von Velsen, Bernhard Rupp, Matthew W Bowler

December 2019 Acta Crystallogr D Struct Biol

Synopsis of Social media discussions

The posts highlight the significance of the research by emphasizing the novel use of controlled dehydration to stabilize protein structures and the discovery of afamin's capacity for multi-site ligand binding. Words like 'exciting,' 'innovative,' and phrases referencing 'methodology' and 'potential biological implications' demonstrate a tone of interest and perceived importance, especially among the scientific community engaged in structural biology and medical imaging.

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Agreement
 Moderate agreement

Most discussions recognize the significance of the research, with some explicitly praising its contribution to structural biology, indicating general consensus on its importance.

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Interest
 Neutral level of interest

E
Engagement
 Neutral engagement

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Impact
 Neutral impact

Social Mentions

YouTube

2 Videos

Twitter

33 Posts

Metrics

Video Views

218

Total Likes

56

Extended Reach

248,761

Social Features

35

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Structural Insights into Afamin's Flexibility and MRI Contrast Binding

Structural Insights into Afamin's Flexibility and MRI Contrast Binding

This study examines the structural challenges of afamin, a human plasma glycoprotein, due to its variable packing and flexibility. Controlled dehydration stabilizes crystal forms and reveals its capacity to bind gadolinium MRI contrast agents at multiple sites.

International Union of Crystallography

December 27, 2019

110 views

Structural Insights into Afamin's Flexibility and MRI Contrast Binding

Structural Insights into Afamin's Flexibility and MRI Contrast Binding

This study investigates the structural challenges of determining afamin's crystal structure due to its variable packing, glycosylation, and conformational flexibility. Controlled dehydration stabilizes a specific crystal form and reveals afamin's ligand-binding capacities, including gadoteridol for MRI applications.

International Union of Crystallography

December 27, 2019

108 views

  • bioRxiv Published
    @biorxiv_pubd (Twitter)

    Controlled dehydration, structural flexibility, and Gadolinium MRI contrast compound binding in human plasma glycoprotein afamin published as: https://t.co/rbB70PUO2A #Acta_Crystallographica_Section_D_Structural_Biology #biorxiv
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    March 15, 2022

  • Analio Dugarte
    @analioj (Twitter)

    RT @ActaCrystD: Naschberger et al.: Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the huma…
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    December 11, 2019

    4

  • Analio Dugarte
    @analioj (Twitter)

    RT @ID30_MASSIF1: New pub @ID30_MASSIF1 in @ActaCrystD from @embl Grenoble and @imed_tweets Controlled dehydration, structural flexibility…
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    December 11, 2019

    6

  • Analio Dugarte
    @analioj (Twitter)

    RT @moncourvoisier: Our preprint @biorxivpreprint with @ruppweb describing dehydration of afamin and how it binds the MRI contrast agent ga…
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    December 11, 2019

    3

  • Veronica KaneDickson
    @vkd002 (Twitter)

    RT @moncourvoisier: Pleased to see our X-ray methods paper
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    December 5, 2019

    7

  • Analio Dugarte
    @analioj (Twitter)

    RT @moncourvoisier: Pleased to see our X-ray methods paper
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    December 4, 2019

    7

  • Helen Gavaghan. UK. Photo+Digital Art by me.
    @HelenGavaghan (Twitter)

    RT @moncourvoisier: Pleased to see our X-ray methods paper
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    December 4, 2019

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  • MASSIF1
    @ID30_MASSIF1 (Twitter)

    RT @moncourvoisier: Pleased to see our X-ray methods paper
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    December 4, 2019

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  • David Bowler
    @MillionAtomMan (Twitter)

    RT @moncourvoisier: Pleased to see our X-ray methods paper
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    December 4, 2019

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  • Structural Biology
    @ActaCrystD (Twitter)

    RT @moncourvoisier: Pleased to see our X-ray methods paper
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    December 4, 2019

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  • Matthew Bowler
    @moncourvoisier (Twitter)

    Pleased to see our X-ray methods paper
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    December 4, 2019

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  • MASSIF1
    @ID30_MASSIF1 (Twitter)

    RT @AfSynchrotron: Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycopro…
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    December 3, 2019

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  • Matthew Bowler
    @moncourvoisier (Twitter)

    RT @AfSynchrotron: Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycopro…
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    December 3, 2019

    2

  • African Synchrotron
    @AfSynchrotron (Twitter)

    Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycoprotein afamin https://t.co/o0Z4xXVOoB via @ActaCrystD https://t.co/X5HhJtEuWN
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    December 2, 2019

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  • Life & Health Science Cluster Tirol
    @lhsct_at (Twitter)

    RT @ID30_MASSIF1: New pub @ID30_MASSIF1 in @ActaCrystD from @embl Grenoble and @imed_tweets Controlled dehydration, structural flexibility…
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    November 28, 2019

    6

  • Structural Biology
    @ActaCrystD (Twitter)

    RT @moncourvoisier: Our preprint @biorxivpreprint with @ruppweb describing dehydration of afamin and how it binds the MRI contrast agent ga…
    view full post

    November 27, 2019

    3

  • MASSIF1
    @ID30_MASSIF1 (Twitter)

    RT @moncourvoisier: Our preprint @biorxivpreprint with @ruppweb describing dehydration of afamin and how it binds the MRI contrast agent ga…
    view full post

    November 27, 2019

    3

  • Matthew Bowler
    @moncourvoisier (Twitter)

    Our preprint @biorxivpreprint with @ruppweb describing dehydration of afamin and how it binds the MRI contrast agent gadolinium is now published @ActaCrystD congrats to @PaulineJYX and @jvonvelsen ! Data collection @ID30_MASSIF1 @esrfsynchrotron @embl
    view full post

    November 27, 2019

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  • Structural Biology
    @ActaCrystD (Twitter)

    RT @ID30_MASSIF1: New pub @ID30_MASSIF1 in @ActaCrystD from @embl Grenoble and @imed_tweets Controlled dehydration, structural flexibility…
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    November 26, 2019

    6

  • Matthew Bowler
    @moncourvoisier (Twitter)

    RT @ID30_MASSIF1: New pub @ID30_MASSIF1 in @ActaCrystD from @embl Grenoble and @imed_tweets Controlled dehydration, structural flexibility…
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    November 26, 2019

    6

  • EMBL
    @embl (Twitter)

    RT @ID30_MASSIF1: New pub @ID30_MASSIF1 in @ActaCrystD from @embl Grenoble and @imed_tweets Controlled dehydration, structural flexibility…
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    November 26, 2019

    6

  • MASSIF1
    @ID30_MASSIF1 (Twitter)

    New pub @ID30_MASSIF1 in @ActaCrystD from @embl Grenoble and @imed_tweets Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycoprotein afamin https://t.co/Ztm2yuJaD5 https://t.co/wbTEsIPdm1
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    November 26, 2019

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  • Molecular Dimensions
    @molecularD (Twitter)

    RT @ActaCrystD: Naschberger et al.: Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the huma…
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    November 20, 2019

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  • MASSIF1
    @ID30_MASSIF1 (Twitter)

    RT @ActaCrystD: Naschberger et al.: Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the huma…
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    November 20, 2019

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  • Matthew Bowler
    @moncourvoisier (Twitter)

    RT @ActaCrystD: Naschberger et al.: Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the huma…
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    November 20, 2019

    4

  • Structural Biology
    @ActaCrystD (Twitter)

    Naschberger et al.: Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycoprotein afamin #IUCr https://t.co/utB0Q33c3t
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    November 19, 2019

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  • MASSIF1
    @ID30_MASSIF1 (Twitter)

    RT @glycopreprint: Controlled dehydration, structural flexibility, and Gadolinium MRI contrast compound binding in human plasma glycoprotei…
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    July 1, 2019

    1

  • Matthew Bowler
    @moncourvoisier (Twitter)

    RT @assert_pub: "Controlled dehydration, structural flexibility, and Gadolinium MRI contrast compound binding in.." is the #1 paper on bioR…
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    June 20, 2019

    1

  • Assert Biorxiv
    @assert_pub (Twitter)

    "Controlled dehydration, structural flexibility, and Gadolinium MRI contrast compound binding in.." is the #1 paper on bioRxiv today in biochemistry. Congrats @PaulineJYX @CaptainRupp. See it at -> https://t.co/dIWIj60FNO and https://t.co/N2ULy4JB4n. Please retweet.
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    June 20, 2019

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  • Matthew Bowler
    @moncourvoisier (Twitter)

    RT @biorxivpreprint: Controlled dehydration, structural flexibility, and Gadolinium MRI contrast compound binding in human plasma glycoprot…
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    June 19, 2019

    1

  • glycopreprint
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    Controlled dehydration, structural flexibility, and Gadolinium MRI contrast compound binding in human plasma glycoprotein afamin https://t.co/Nw47HZYlKC #glycotime
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    June 18, 2019

    1

    1

  • bxv_biochem
    @bxv_biochem (Twitter)

    Controlled dehydration, structural flexibility, and Gadolinium MRI contrast compound binding in human plasma glycoprotein afamin https://t.co/pAocKWIpHt
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    June 18, 2019

    2

  • bioRxiv
    @biorxivpreprint (Twitter)

    Controlled dehydration, structural flexibility, and Gadolinium MRI contrast compound binding in human plasma glycoprotein afamin https://t.co/g4X8eGXXWi #bioRxiv
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    June 18, 2019

    2

    1

Abstract Synopsis

  • The study investigates the structural challenges of determining afamin's crystal structure due to its variable packing, glycosylation, and conformational flexibility and demonstrates that controlled dehydration can stabilize a specific crystal form and alter protein conformation.
  • Utilizing automated experiments on MASSIF1, researchers established an efficient method to induce phase transitions, with insights suggesting that relying on a single structural model may lead to interpretative biases, especially regarding ligand binding sites.
  • Afamin, a multifunctional transporter, can bind gadoteridol, a contrast agent used in MRI, at multiple sites, including near the Sudlow site of albumin, highlighting its promiscuous ligand-binding capacity which may influence its biological and imaging functions.]