Optotracing for selective fluorescence-based detection, visualization and quantification of live S. aureus in real-time.

Karen Butina, Ana Tomac, Ferdinand X Choong, Hamid Shirani, K Peter R Nilsson, Susanne Löffler, Agneta Richter-Dahlfors

October 2020

Synopsis of Social media discussions

The discussions reflect a positive and enthusiastic attitude towards the publication, with comments like 'This could be you!' and 'new dynamic detection method' emphasizing the innovative and promising nature of the research. The tone and wording convey excitement about the potential applications and improvements in pathogen detection, highlighting the article's significance in infection research.

A
Agreement
 Moderate agreement

Most discussions praise the article, highlighting its potential and innovative approach, with phrases like 'this could be you!' and 'new dynamic detection method' indicating a positive reception.

I
Interest
 High level of interest

Posts demonstrate high interest by emphasizing the significance of real-time detection and visualization of S. aureus, which is central to infection research and diagnostics.

E
Engagement
 Moderate level of engagement

Participants actively discuss the method's versatility and implications, citing specific features such as selective fluorescence and detection in mixed cultures.

I
Impact
 Moderate level of impact

While the comments are enthusiastic and suggest the technique could transform diagnostics, they mostly focus on potential impact rather than concrete implementation or widespread adoption.

Social Mentions

YouTube

1 Videos

Twitter

10 Posts

Metrics

Video Views

102

Total Likes

7

Extended Reach

15,192

Social Features

11

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Real-Time Fluorescence Detection of Live S. aureus Using Optotracing

Real-Time Fluorescence Detection of Live S. aureus Using Optotracing

This video explains how optotracing technology extends to detect, visualize, and quantify live Staphylococcus aureus bacteria in real time using fluorescence. It highlights the mechanism, specificity, and applications in infection research.

AJE Video Bytes

February 1, 2022

102 views

  • John Bergqvist
    @JohnBergqvist (Twitter)

    RT @EbbaBiotech: This could be you! Check out how EbbaBiolight labels Staph specifically in mixed cultures of Staphylococci and Salmonella…
    view full post

    June 16, 2022

    1

  • Ebba Biotech
    @EbbaBiotech (Twitter)

    This could be you! Check out how EbbaBiolight labels Staph specifically in mixed cultures of Staphylococci and Salmonella
    view full post

    June 16, 2022

    1

    1

  • npj Journals
    @Nature_NPJ (Twitter)

    Optotracing for selective fluorescence-based detection, visualization and quantification of live S. aureus in real-time. #npjbiofilms https://t.co/ypdAUY5qui https://t.co/5XaetHd2K6
    view full post

    November 12, 2020

  • enterococcuspapers
    @entero_papers (Twitter)

    Optotracing for selective fluorescence-based detection, visualization and quantification of live S. aureus in real-time. https://t.co/1PE1gEk37D https://t.co/nNXohf7P35
    view full post

    October 11, 2020

  • Charles Williams, PhD (My views are my own)
    @cwilliamszf (Twitter)

    Optotracing for selective fluorescence-based detection, visualization and quantification of live S. aureus in real-time. https://t.co/83RHIbbWnp
    view full post

    October 11, 2020

  • Nano IJ
    @nanomicroscopy (Twitter)

    Optotracing for selective fluorescence-based detection, visualization and quantification of live S. aureus in real-time. https://t.co/gnKBlccpSh https://t.co/hcDoBUZCIp
    view full post

    October 11, 2020

  • Super Res Microscopy
    @SRMicro_papers (Twitter)

    Optotracing for selective fluorescence-based detection, visualization and quantification of live S. aureus in real-time. by Butina K, Tomac A, Choong FX, Shirani H, Nilsson KPR, Löffler S, Richter-Dahlfors A https://t.co/JbkN6xZP2V
    view full post

    October 11, 2020

  • Ana Tomac
    @Ana__Tomac (Twitter)

    RT @AIMEScenter: New Publication out today in @Nature_NPJ #Biofilms & #Microbiomes "Optotracing for selective fluorescence-based detection,…
    view full post

    October 10, 2020

    2

  • Nicolas Fanget
    @nfanget (Twitter)

    RT @AIMEScenter: New Publication out today in @Nature_NPJ #Biofilms & #Microbiomes "Optotracing for selective fluorescence-based detection,…
    view full post

    October 10, 2020

    2

  • AIMES
    @AIMEScenter (Twitter)

    New Publication out today in @Nature_NPJ #Biofilms & #Microbiomes "Optotracing for selective fluorescence-based detection, visualization and quantification of live S. aureus in real-time" We show our new dynamic detection method for S. aureus https://t.co/cZwhw9TaU8 https://t.co/nI6guh9X9T
    view full post

    October 9, 2020

    4

    2

Abstract Synopsis

  • The study extends optotracing technology, originally used for gram-negative bacteria, to specifically detect and visualize the gram-positive pathogen Staphylococcus aureus in real time.
  • A specially designed optotracer was identified that binds to S. aureus, producing fluorescence, which allows for its detection, quantification, and visualization, even in mixed bacterial samples with gram-negative Salmonella Enteritidis.
  • The binding mechanism is primarily driven by hydrophobic interactions, involving the bacterial cell envelope, and can be selectively tuned by adjusting buffer conditions, making optotracing a versatile tool for infection research and diagnostics.