Acta Crystallogr D Struct Biol November 2020 PMID 33135680

Through-grid wicking enables high-speed cryoEM specimen preparation.

Yong Zi Tan, John L Rubinstein

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Peak: 17 in May 2020

across 2 sources since May 2020

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The discussions largely praise the publication's contribution to cryoEM, with phrases like 'enables high-speed specimen prep' and 'significant improvement' indicating strong approval and interest. Examples like references to datasets and mentions of methodological advantages demonstrate active engagement and recognition of the technique's potential impact.

AgreementModerate agreement

Most discussions express support or acknowledgment of the article's significance, reflecting a general consensus on its importance in cryoEM methods.

InterestHigh interest

Posts are highly interested, as indicated by emphasis on key advantages and continual referencing of the publication and related datasets.

EngagementModerate engagement

Comments show moderate engagement, with some sharing additional information and highlighting the technique's benefits, but few deep discussions.

ImpactModerate impact

Several posts highlight the potential for this method to influence future research, suggesting a notable impact on the field.

Abstract in brief

Throughgrid wicking allows for rapid cryoEM specimen preparation by using a glassfiber filter to wick solution through a grid in tens of milliseconds, reducing problems like specimen damage and preferred orientations.
The method combines ultrasonic specimen application with throughgrid wicking in a device called Backitup (BIU), making it less sensitive to sample volume and improving ice quality over larger areas for both soluble proteins and detergent-solubilized complexes.
The increased speed of this process enhances the number of usable views of a specimen, aiding in high-resolution cryoEM studies and advancing time-resolved imaging.]

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Rapid CryoEM Sample Preparation Using Through-Grid Wicking

Through-grid wicking allows for rapid cryoEM specimen preparation by using a glassfiber filter to wick solution in tens of milliseconds, reducing damage and preferred orientations. The process improves ice quality and…

International Union of Crystallography · Apr 15, 2021 392 views

High-Speed CryoEM Sample Preparation Using Through-Grid Wicking

Through-grid wicking allows rapid cryoEM specimen preparation by using a glassfiber filter to wick solution through the grid in tens of milliseconds. This method reduces specimen damage and preferred orientations…

International Union of Crystallography · Apr 15, 2021 345 views

bioRxiv Published

@biorxiv_pubd · X

Through-grid wicking enables high-speed cryoEM specimen preparation published as: https://t.co/Lx2N6RnjGr #Acta_Crystallographica_Section_D_Structural_Biology #biorxiv

Jul 12, 2024 · 2 likes · 2,908 followers View post →

Yuhe Renee Yang

@ReneeYuheYang · X

RT @ActaCrystD: Tan & Rubinstein: Through-grid wicking enables high-speed cryoEM specimen preparation #CryoEM #SpecimenPreparation #Backitu…

Sep 26, 2022 · 6 shares · 391 followers View post →

EMDB - EMPIAR @EBI

@EMDB_EMPIAR · X

The dataset for the reconstruction of this #influenza hemagglutinin protein is available at #EMPIAR right now. This #singleparticle dataset produce a structure was resolved to 2.9 Å. DOI: https://t.co/sU1sTb5iUO EMPIAR: https://t.co/C7HJp3BW4w EMDB: https://t.co/C7HJp3BW4w

Sep 9, 2021 · 6,117 followers View post →

Shinya Fushinobu

@sugargroove · X

Through-grid wickingとは Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Kxgessn922 https://t.co/wTGqSrs1I2

Nov 5, 2020 · 1 like · 4,147 followers View post →

Vikas Navratna

@vikasnavaratna · X

RT @ActaCrystD: Tan & Rubinstein: Through-grid wicking enables high-speed cryoEM specimen preparation #CryoEM #SpecimenPreparation #Backitu…

Oct 15, 2020 · 6 shares · 5,404 followers View post →

Nick Schnicker

@n_schnicker · X

RT @ActaCrystD: Tan & Rubinstein: Through-grid wicking enables high-speed cryoEM specimen preparation #CryoEM #SpecimenPreparation #Backitu…

Oct 14, 2020 · 6 shares · 537 followers View post →

Maier Lab

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RT @ActaCrystD: Tan & Rubinstein: Through-grid wicking enables high-speed cryoEM specimen preparation #CryoEM #SpecimenPreparation #Backitu…

Oct 14, 2020 · 6 shares · 1,043 followers View post →

Max Maletta

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RT @ActaCrystD: Tan & Rubinstein: Through-grid wicking enables high-speed cryoEM specimen preparation #CryoEM #SpecimenPreparation #Backitu…

Oct 14, 2020 · 6 shares · 521 followers View post →

Sriram Shreedhar

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RT @ActaCrystD: Tan & Rubinstein: Through-grid wicking enables high-speed cryoEM specimen preparation #CryoEM #SpecimenPreparation #Backitu…

Oct 14, 2020 · 6 shares · 38 followers View post →

BreakthroughMedicine&AdvancedResearchTools

@JayChance12 · X

RT @rnomics: Top #tweeted story in #structbio: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/stQizUEEn0,…

Oct 13, 2020 · 1 share · 633 followers View post →

Fabrice Leclerc

@rnomics · X

Top #tweeted story in #structbio: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/stQizUEEn0, see more https://t.co/ThsI8L7UXY

Oct 13, 2020 · 2 likes · 1 share · 2,757 followers View post →

Structural Biology

@ActaCrystD · X

Tan & Rubinstein: Through-grid wicking enables high-speed cryoEM specimen preparation #CryoEM #SpecimenPreparation #Backitup @uoft ... #IUCr https://t.co/IRJ8PgpWvj

Oct 13, 2020 · 17 likes · 6 shares · 3,023 followers View post →

MiTeGen

@MiTeGen · X

Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/KW5zPQyYTF - Authors Yong Zi Tan and John Rubinstein

Aug 7, 2020 · 2,908 followers View post →

Saif Khan

@sfk_usc · X

RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 16, 2020 · 14 shares · 121 followers View post →

S. Saif Hasan

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RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 9, 2020 · 14 shares · 243 followers View post →

Mathieu Rappas

@matrappas · X

Through-grid wicking enables high-speed cryoEM specimen preparation | bioRxiv https://t.co/ml34u31s1w

May 6, 2020 · 110 followers View post →

Max Maletta

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RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 5, 2020 · 14 shares · 521 followers View post →

dapeng

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RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

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Yan Han

@YanHan914 · X

RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 4, 2020 · 14 shares · 40 followers View post →

Ci Ji Lim

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RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 4, 2020 · 14 shares · 1,424 followers View post →

CryoGourmet Fan

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RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 4, 2020 · 14 shares · 463 followers View post →

やし

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RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 4, 2020 · 14 shares · 357 followers View post →

Dominika Borek

@dmborek · X

RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 4, 2020 · 14 shares · 649 followers View post →

Da Cui 崔达

@cuida1991 · X

RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 4, 2020 · 14 shares · 536 followers View post →

Alexander Sobolevsky

@AlexanderSobol6 · X

RT @cryoEM_Papers: Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

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bxv_biophys

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Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/bRs4YkPxJZ

May 4, 2020 · 562 followers View post →

223

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cryoEM Papers

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Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Md5GXDYxxu

May 4, 2020 · 37 likes · 14 shares · 11,812 followers View post →

bioRxiv Biophysics

@biorxiv_biophys · X

Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/Dg3fsjfQBT #biorxiv_biophys

May 4, 2020 · 2 likes · 5,437 followers View post →

bioRxiv

@biorxivpreprint · X

Through-grid wicking enables high-speed cryoEM specimen preparation https://t.co/apqFyUzEm9 #bioRxiv

May 4, 2020 · 2 likes · 138,159 followers View post →

Through-grid wicking enables high-speed cryoEM specimen preparation.

Mentions per month

Where it's discussed

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Abstract in brief

Top posts

All mentions

Rapid CryoEM Sample Preparation Using Through-Grid Wicking

High-Speed CryoEM Sample Preparation Using Through-Grid Wicking