Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes.

Jennifer A Taylor, Cintia C Santiago, Joe Gray, Kimberly A Wodzanowski, Kristen E DeMeester, Jacob Biboy, Waldemar Vollmer, Catherine L Grimes, Nina R Salama

April 2021 Curr Protoc

Synopsis of Social media discussions

The discussions reflect an overall positive reception, with posts praising the sharing of methods and acknowledgments of collaborative work, such as thanks given to a collaborator for making protocols accessible; the tone is appreciative but does not delve into detailed scientific critique, indicating moderate agreement and impact.

A
Agreement
 Moderate agreement

Most discussions express approval and appreciation for the research team's work and publication, indicating general agreement with the importance of the findings.

I
Interest
 Moderate level of interest

Comments show moderate curiosity and acknowledgment of the methods used, but lack detailed critical analysis, suggesting a solid but not highly enthusiastic interest.

E
Engagement
 Neutral engagement

While some posts thank collaborators and mention protocols, there is limited in-depth discussion or debate, reflecting a neutral level of engagement.

I
Impact
 Moderate level of impact

The emphasis on method dissemination and collaborative efforts suggests that the research could have meaningful influence in the field, but the discussions don't firmly establish its broader impact.

Social Mentions

YouTube

6 Videos

Twitter

8 Posts

Metrics

Video Views

1,623

Total Likes

18

Extended Reach

52,860

Social Features

14

Timeline: Posts about article

Top Social Media Posts

Posts referencing the article

Visualizing Peptidoglycan Synthesis in Helicobacter pylori Using Clickable Probes

Visualizing Peptidoglycan Synthesis in Helicobacter pylori Using Clickable Probes

This video discusses protocols for studying peptidoglycan cell wall synthesis in Helicobacter pylori using MurNAcalk probes, advanced microscopy, and mass spectrometry techniques.

CurrentProtocols

February 11, 2022

1,266 views

Protocols for Visualizing Peptidoglycan Synthesis in Helicobacter pylori

Protocols for Visualizing Peptidoglycan Synthesis in Helicobacter pylori

This video demonstrates protocols for studying peptidoglycan cell wall synthesis in Helicobacter pylori using clickable metabolic probes and advanced microscopy techniques, including steps for bacterial growth, labeling, and visualization of cell wall structure.

CurrentProtocols

February 11, 2022

198 views

Disassembling Cytocentrifuge Units for Analysis and Maintenance

Disassembling Cytocentrifuge Units for Analysis and Maintenance

This video provides a detailed guide to disassembling cytocentrifuge units, focusing on proper techniques for analysis and maintenance. For more information, see the protocol on localizing peptidoglycan synthesis in Helicobacter pylori using clickable metabolic probes.

CurrentProtocols

February 11, 2022

54 views

Advanced Protocols for Visualizing Peptidoglycan Synthesis in Helicobacter pylori

Advanced Protocols for Visualizing Peptidoglycan Synthesis in Helicobacter pylori

This video introduces new tools and protocols for studying peptidoglycan cell wall synthesis in Helicobacter pylori using clickable metabolic probes, enhancing visualization with advanced microscopy techniques.

CurrentProtocols

February 11, 2022

41 views

Protocol for Visualizing Peptidoglycan Synthesis in Helicobacter pylori

Protocol for Visualizing Peptidoglycan Synthesis in Helicobacter pylori

This video covers protocols for studying peptidoglycan cell wall synthesis in Helicobacter pylori using clickable metabolic probes like MurNAcalk, combined with advanced microscopy techniques to visualize and analyze bacterial cell wall synthesis.

CurrentProtocols

February 11, 2022

36 views

Localization of Peptidoglycan Synthesis in Helicobacter pylori Using Clickable Probes

Localization of Peptidoglycan Synthesis in Helicobacter pylori Using Clickable Probes

This video discusses protocols for localizing peptidoglycan synthesis in Helicobacter pylori using specific sugar probes like MurNAcalk, enabling detailed visualization of bacterial cell wall synthesis through advanced microscopy techniques.

CurrentProtocols

February 11, 2022

29 views

  • Dr. Kimberly Wodzanowski
    @KAWodzanowski (Twitter)

    RT @PyloriNina: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big tha…
    view full post

    April 23, 2021

    3

  • Fred Hutch Cancer Center
    @fredhutch (Twitter)

    RT @PyloriNina: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big tha…
    view full post

    April 23, 2021

    3

  • Current Protocols
    @Curr_Protocols (Twitter)

    RT @PyloriNina: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big tha…
    view full post

    April 23, 2021

    3

  • Dr. Nina Salama
    @PyloriNina (Twitter)

    Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big thanks to @j_a_tay for corralling our awesome collaborators that made her thesis possible to get all the methods public with @CurrentProtocol 1/3
    view full post

    April 23, 2021

    14

    3

  • Your HPLC DataBase
    @LC_Analysis (Twitter)

    Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/XWSKIlhhZ1
    view full post

    April 19, 2021

  • David I Roper
    @davidroper2 (Twitter)

    RT @GlcNAcMurNAc: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/WUT5u6j75U
    view full post

    April 14, 2021

    1

  • PeptidoglycanPapers
    @GlcNAcMurNAc (Twitter)

    Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/WUT5u6j75U
    view full post

    April 13, 2021

    2

    1

  • Super Res Microscopy
    @SRMicro_papers (Twitter)

    Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes by Jennifer A Taylor https://t.co/dxywIkg0QV
    view full post

    April 13, 2021

Abstract Synopsis

  • The article discusses new tools and protocols for studying peptidoglycan (PG) cell wall synthesis in Helicobacter pylori using a specialized sugar probe called MurNAcalk, which can be visualized with advanced microscopy techniques.
  • It introduces a novel metabolic pathway for producing MurNAcalk, along with methods like mass spectrometry and structured illumination microscopy (SIM) to analyze and visualize PG synthesis and muropeptide composition.
  • The protocols include steps for growing H. pylori, labeling with MurNAcalk, analyzing the incorporation of the probe into PG, and visualizing the bacteria's cell wall structure in detail.]