Curr Protoc April 2021 PMID 33844460

Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes.

Jennifer A Taylor, Cintia C Santiago, Joe Gray, Kimberly A Wodzanowski, Kristen E DeMeester, Jacob Biboy, Waldemar Vollmer, Catherine L Grimes, Nina R Salama

Mentions

14
Peak: 8 in Apr 2021
across 2 sources since Apr 2021

Extended reach

52,860
potential audience

Total likes

18

Video views

1,623

Mentions per month

Apr 2021 – Feb 2022

Where it's discussed

  • X / Twitter8 posts
  • YouTube6 videos

AI synopsis

What people are saying

The discussions reflect an overall positive reception, with posts praising the sharing of methods and acknowledgments of collaborative work, such as thanks given to a collaborator for making protocols accessible; the tone is appreciative but does not delve into detailed scientific critique, indicating moderate agreement and impact.

AgreementModerate agreement

Most discussions express approval and appreciation for the research team's work and publication, indicating general agreement with the importance of the findings.

InterestModerate interest

Comments show moderate curiosity and acknowledgment of the methods used, but lack detailed critical analysis, suggesting a solid but not highly enthusiastic interest.

EngagementNeutral engagement

While some posts thank collaborators and mention protocols, there is limited in-depth discussion or debate, reflecting a neutral level of engagement.

ImpactModerate impact

The emphasis on method dissemination and collaborative efforts suggests that the research could have meaningful influence in the field, but the discussions don't firmly establish its broader impact.

Abstract in brief

  • The article discusses new tools and protocols for studying peptidoglycan (PG) cell wall synthesis in Helicobacter pylori using a specialized sugar probe called MurNAcalk, which can be visualized with advanced microscopy techniques.
  • It introduces a novel metabolic pathway for producing MurNAcalk, along with methods like mass spectrometry and structured illumination microscopy (SIM) to analyze and visualize PG synthesis and muropeptide composition.
  • The protocols include steps for growing H. pylori, labeling with MurNAcalk, analyzing the incorporation of the probe into PG, and visualizing the bacteria's cell wall structure in detail.]

Top posts

Most liked on X and Bluesky
Dr. Nina Salama
@PyloriNina · X

Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big thanks to @j_a_tay for corralling our awesome collaborators that made her thesis possible to get all the methods public with @CurrentProtocol 1/3

Apr 23, 2021 · 14 likes · 3 shares · 638 followers View post →
PeptidoglycanPapers
@GlcNAcMurNAc · X

Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/WUT5u6j75U

Apr 13, 2021 · 2 likes · 1 share · 847 followers View post →
Fred Hutch Cancer Center
@fredhutch · X

RT @PyloriNina: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big tha…

Apr 23, 2021 · 3 shares · 38,459 followers View post →
Your HPLC DataBase
@LC_Analysis · X

Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/XWSKIlhhZ1

Apr 19, 2021 · 3,545 followers View post →
Super Res Microscopy
@SRMicro_papers · X

Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes by Jennifer A Taylor https://t.co/dxywIkg0QV

Apr 13, 2021 · 3,119 followers View post →
David I Roper
@davidroper2 · X

RT @GlcNAcMurNAc: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/WUT5u6j75U

Apr 14, 2021 · 1 share · 2,196 followers View post →

All mentions

Dr. Kimberly Wodzanowski
@KAWodzanowski · X

RT @PyloriNina: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big tha…

Apr 23, 2021 · 3 shares · 211 followers View post →
Fred Hutch Cancer Center
@fredhutch · X

RT @PyloriNina: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big tha…

Apr 23, 2021 · 3 shares · 38,459 followers View post →
Current Protocols
@Curr_Protocols · X

RT @PyloriNina: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big tha…

Apr 23, 2021 · 3 shares · 2,195 followers View post →
Dr. Nina Salama
@PyloriNina · X

Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/N0UwFRCvkG Big thanks to @j_a_tay for corralling our awesome collaborators that made her thesis possible to get all the methods public with @CurrentProtocol 1/3

Apr 23, 2021 · 14 likes · 3 shares · 638 followers View post →
Your HPLC DataBase
@LC_Analysis · X

Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/XWSKIlhhZ1

Apr 19, 2021 · 3,545 followers View post →
David I Roper
@davidroper2 · X

RT @GlcNAcMurNAc: Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/WUT5u6j75U

Apr 14, 2021 · 1 share · 2,196 followers View post →
PeptidoglycanPapers
@GlcNAcMurNAc · X

Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes https://t.co/WUT5u6j75U

Apr 13, 2021 · 2 likes · 1 share · 847 followers View post →
Super Res Microscopy
@SRMicro_papers · X

Localizing Peptidoglycan Synthesis in Helicobacter pylori using Clickable Metabolic Probes by Jennifer A Taylor https://t.co/dxywIkg0QV

Apr 13, 2021 · 3,119 followers View post →