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Posts referencing the article
Controlling Multigene Expression Ratios in Mammalian Cells Using Synthetic Promoters
Control of multigene expression using synthetic promoters in mammalian cells, highlighting the importance of empirical testing due to variability in gene expression outcomes related to vector context and gene position.
October 5, 2025
561 views
Controlling Gene Expression Ratios in Mammalian Cells Using Synthetic Promoters
Synthetic promoters can manage gene expression ratios in mammalian cells, but actual outcomes often differ from predictions. Empirical testing is essential to account for vector context and gene position effects.
October 7, 2025
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October 24, 2025
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CHO Cell Papers
@CHOculturePaper (Twitter)October 22, 2025
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Biotechnology Journal - 2025 - Szkodny - A Flexible Hybrid Site ...
5 (2021): 1155–1165, https://doi.org/10.1021/acssynbio. 3. L. M. Barnes, C. M. Bentley, and A. J. Dickson, “Stability of Protein 0c00643. Production From ...
view full postDecember 14, 2025
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Abstract Synopsis
- Synthetic promoters with different levels of activity can be used to control the ratio of multiple genes expressed in mammalian cells, but actual gene expression often deviates from predictions.
- Expression of all genes in multigene vectors is typically reduced compared to when the same genes are expressed individually, and gene position within the vector influences how much each gene is expressed.
- While synthetic promoters are useful for designing multigene vectors with desired expression ratios, empirical testing is essential because factors like vector context and gene position can significantly affect gene expression outcomes.]
CHO Cell Papers
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