Control of Multigene Expression Stoichiometry in Mammalian Cells Using Synthetic Promoters.

Abstract in brief

• Synthetic promoters with different levels of activity can be used to control the ratio of multiple genes expressed in mammalian cells, but actual gene expression often deviates from predictions.
• Expression of all genes in multigene vectors is typically reduced compared to when the same genes are expressed individually, and gene position within the vector influences how much each gene is expressed.
• While synthetic promoters are useful for designing multigene vectors with desired expression ratios, empirical testing is essential because factors like vector context and gene position can significantly affect gene expression outcomes.]