Synopsis of Social media discussions
The group shows strong support and interest in the publication, exemplified by phrases like 'Our protocol has been published!' and expressions of appreciation for the authors and contributors. The tone is enthusiastic and appreciative, emphasizing the practical utility and accessibility of the work, which supports the moderate engagement and impact scores.
Agreement
Moderate agreementMost discussions express enthusiasm and support for the publication and its protocols, indicating general agreement with its value.
Interest
High level of interestThe frequent use of positive phrases like 'check it out' and 'published' suggests high interest among the community.
Engagement
Moderate level of engagementPosts include enthusiasm for sharing protocols and thanking authors, showing moderate engagement with both content and its implications.
Impact
Moderate level of impactWhile some posts highlight the significance of the protocols and their open-access nature, the overall impact perceived remains moderate, focused on dissemination rather than transformative change.
Social Mentions
YouTube
7 Videos
6 Posts
Metrics
Video Views
20,909
Total Likes
137
Extended Reach
25,496
Social Features
13
Timeline: Posts about article
Top Social Media Posts
Current Protocols
Here, and through comprehensive step-by-step protocols, the authors (@Malaghan_Inst) aim to guide users through the theory of optimizing a high-dimensional full-spectrum #FlowCytometry panel for #immunophenotyping.
➡️https://t.co/WX2AESdhOy #OpenAccess https://t.co/tmF7L9uRCz
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Laura Ferrer
Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC
Thanks to all the authors and @CytekBio!
#FlowCytometry #Immunophenotyping
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Malaghan Institute
RT @CurrentProtocol:
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ACS- Australasian Cytometry Society
RT @Lauretalila: Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC…
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Brittany Lavender
RT @Lauretalila: Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC…
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Posts referencing the article
High-Dimensional Spectral Flow Cytometry Panel Design and Optimization Techniques
This video provides a detailed overview of designing and optimizing spectral flow cytometry panels, covering fluorophore selection, spillover management, and testing for accurate high-dimensional immune cell analysis. It includes practical protocols and troubleshooting tips.
July 26, 2021
11,489 views
Optimizing High-Dimensional Immunophenotyping Panels with Full Spectrum Flow Cytometry
This video discusses how full-spectrum flow cytometry enables larger, more complex immune cell panels, offering protocols for optimization and troubleshooting to improve high-dimensional data analysis.
April 25, 2022
5,142 views
Optimizing High-Dimensional Immunophenotyping with Full-Spectrum Flow Cytometry
This video discusses how advancements in full-spectrum flow cytometry enable larger immune cell panels and offers protocols for optimizing high-dimensional flow cytometry panels, including practical examples and troubleshooting methods.
April 25, 2022
1,124 views
Optimizing High-Dimensional Flow Cytometry Panels for Accurate Data Analysis
This video discusses advancements in full-spectrum flow cytometry that enable larger immune cell panels and provides step-by-step protocols for panel optimization, including practical examples and troubleshooting techniques.
April 25, 2022
995 views
Evaluation of Fully Stained Sample for Flow Cytometry Analysis
This video discusses methods for evaluating fully stained samples in high-dimensional flow cytometry, based on protocols for optimizing immune cell panels using full-spectrum flow cytometry, which enables detailed immune profiling with complex marker panels.
April 25, 2022
905 views
Evaluating Data Quality in High-Dimensional Flow Cytometry Using Expert Guidance
This video discusses protocols for optimizing and assessing data quality in complex immune cell analysis using full-spectrum flow cytometry.
April 25, 2022
815 views
Optimizing High-Dimensional Immunophenotyping with Full-Spectrum Flow Cytometry
This video discusses the optimization of complex immune cell panels using full-spectrum flow cytometry, enabling the analysis of up to 43 colors for accurate high-dimensional data. It includes step-by-step protocols and practical examples.
April 25, 2022
441 views
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RT @CurrentProtocol:
view full postDecember 31, 2021
4
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Sam Small
@SamSmallNZ (Twitter)RT @CurrentProtocol:
view full postDecember 31, 2021
4
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Current Protocols
@Curr_Protocols (Twitter)Here, and through comprehensive step-by-step protocols, the authors (@Malaghan_Inst) aim to guide users through the theory of optimizing a high-dimensional full-spectrum #FlowCytometry panel for #immunophenotyping. ➡️https://t.co/WX2AESdhOy #OpenAccess https://t.co/tmF7L9uRCz
view full postSeptember 13, 2021
11
6
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ACS- Australasian Cytometry Society
@ACScytometry (Twitter)RT @Lauretalila: Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC…
view full postSeptember 7, 2021
3
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Brittany Lavender
@gingersnapsci (Twitter)RT @Lauretalila: Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC…
view full postSeptember 7, 2021
3
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Laura Ferrer
@Lauretalila (Twitter)Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC Thanks to all the authors and @CytekBio! #FlowCytometry #Immunophenotyping
view full postSeptember 7, 2021
5
3
Abstract Synopsis
- This article discusses how advancements in full-spectrum flow cytometry have enabled the creation of larger, more complex immune cell panels (up to 43 colors) but also introduce challenges in designing and optimizing these panels for accurate high-dimensional data analysis.
- Full-spectrum flow cytometry measures the entire emission spectrum of each fluorophore, making it easier to use fluorophores with similar emission peaks but different spectral fingerprints, which facilitates the design of larger panels.
- The paper provides step-by-step protocols for optimizing high-dimensional flow cytometry panels, including practical examples like a 24-color panel for identifying T-cell subsets, along with troubleshooting methods to address common issues in panel design and setup.]
Malaghan Institute
@Malaghan_Inst (Twitter)