Curr Protoc September 2021 PMID 34492732

Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry.

Laura Ferrer-Font, Sam J Small, Brittany Lewer, Katherine R Pilkington, Laura K Johnston, Lily M Park, Joanne Lannigan, Maria C Jaimes, Kylie M Price

Mentions

13
Peak: 6 in Apr 2022
across 2 sources since Jul 2021

Extended reach

25,496
potential audience

Total likes

137

Video views

20,909

Mentions per month

Jul 2021 – Apr 2022

Where it's discussed

  • YouTube7 videos
  • X / Twitter6 posts

AI synopsis

What people are saying

The group shows strong support and interest in the publication, exemplified by phrases like 'Our protocol has been published!' and expressions of appreciation for the authors and contributors. The tone is enthusiastic and appreciative, emphasizing the practical utility and accessibility of the work, which supports the moderate engagement and impact scores.

AgreementModerate agreement

Most discussions express enthusiasm and support for the publication and its protocols, indicating general agreement with its value.

InterestHigh interest

The frequent use of positive phrases like 'check it out' and 'published' suggests high interest among the community.

EngagementModerate engagement

Posts include enthusiasm for sharing protocols and thanking authors, showing moderate engagement with both content and its implications.

ImpactModerate impact

While some posts highlight the significance of the protocols and their open-access nature, the overall impact perceived remains moderate, focused on dissemination rather than transformative change.

Abstract in brief

  • This article discusses how advancements in full-spectrum flow cytometry have enabled the creation of larger, more complex immune cell panels (up to 43 colors) but also introduce challenges in designing and optimizing these panels for accurate high-dimensional data analysis.
  • Full-spectrum flow cytometry measures the entire emission spectrum of each fluorophore, making it easier to use fluorophores with similar emission peaks but different spectral fingerprints, which facilitates the design of larger panels.
  • The paper provides step-by-step protocols for optimizing high-dimensional flow cytometry panels, including practical examples like a 24-color panel for identifying T-cell subsets, along with troubleshooting methods to address common issues in panel design and setup.]

Top posts

Most liked on X and Bluesky
Current Protocols
@Curr_Protocols · X

Here, and through comprehensive step-by-step protocols, the authors (@Malaghan_Inst) aim to guide users through the theory of optimizing a high-dimensional full-spectrum #FlowCytometry panel for #immunophenotyping. ➡️https://t.co/WX2AESdhOy #OpenAccess https://t.co/tmF7L9uRCz

Sep 13, 2021 · 11 likes · 6 shares · 2,187 followers View post →
Laura Ferrer
@Lauretalila · X

Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC Thanks to all the authors and @CytekBio! #FlowCytometry #Immunophenotyping

Sep 7, 2021 · 5 likes · 3 shares · 331 followers View post →
Malaghan Institute
@Malaghan_Inst · X

RT @CurrentProtocol:

Dec 31, 2021 · 4 shares · 1,263 followers View post →
ACS- Australasian Cytometry Society
@ACScytometry · X

RT @Lauretalila: Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC…

Sep 7, 2021 · 3 shares · 678 followers View post →
Brittany Lavender
@gingersnapsci · X

RT @Lauretalila: Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC…

Sep 7, 2021 · 3 shares · 56 followers View post →
Sam Small
@SamSmallNZ · X

RT @CurrentProtocol:

Dec 31, 2021 · 4 shares · 29 followers View post →

All mentions

Malaghan Institute
@Malaghan_Inst · X

RT @CurrentProtocol:

Dec 31, 2021 · 4 shares · 1,263 followers View post →
Sam Small
@SamSmallNZ · X

RT @CurrentProtocol:

Dec 31, 2021 · 4 shares · 29 followers View post →
Current Protocols
@Curr_Protocols · X

Here, and through comprehensive step-by-step protocols, the authors (@Malaghan_Inst) aim to guide users through the theory of optimizing a high-dimensional full-spectrum #FlowCytometry panel for #immunophenotyping. ➡️https://t.co/WX2AESdhOy #OpenAccess https://t.co/tmF7L9uRCz

Sep 13, 2021 · 11 likes · 6 shares · 2,187 followers View post →
ACS- Australasian Cytometry Society
@ACScytometry · X

RT @Lauretalila: Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC…

Sep 7, 2021 · 3 shares · 678 followers View post →
Brittany Lavender
@gingersnapsci · X

RT @Lauretalila: Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC…

Sep 7, 2021 · 3 shares · 56 followers View post →
Laura Ferrer
@Lauretalila · X

Our protocol for Full Spectrum Flow Cytometry Panel Optimisation has been published! Check it out! https://t.co/xU8ndYMYGC Thanks to all the authors and @CytekBio! #FlowCytometry #Immunophenotyping

Sep 7, 2021 · 5 likes · 3 shares · 331 followers View post →