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Plasmid May 2022 PMID 35134433

Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures.

Ileana Tossolini, Agustina Gugliotta, Fernando López Díaz, Ricardo Kratje, Claudio Prieto

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Mentions

10
Peak: 7 in Oct 2021
across 3 sources since Oct 2021

Extended reach

147,998
potential audience

Total likes

10

Video views

156

Mentions per month

Oct 2021 – Dec 2025

Where it's discussed

  • X / Twitter7 posts
  • YouTube2 videos
  • News1 articles

AI synopsis

What people are saying

The discussions highlight the importance of the study, with phrases like 'promising approach' and mentions of its relevance to industrial protein production. The tone indicates a balanced appreciation of its scientific contributions, emphasizing the potential for stable gene expression and reduction of cellular stress, while casual mentions like ‘RT’ and hashtags show a general interest in disseminating the findings.

AgreementModerate agreement

Most discussions acknowledge the potential and significance of the research, viewing it positively for its implications.

InterestModerate interest

Participants demonstrate moderate interest, with repeated mentions of the study's focus and relevance.

EngagementModerate engagement

Comments reflect a moderate level of engagement, referencing the study's methodology and findings, though without deep technical elaboration.

ImpactModerate impact

The discussions suggest an awareness of the study's moderate impact on fields like bioproduction and cell engineering but do not emphasize transformative changes.

Abstract in brief

  • The study explores using endogenous promoters from CHOK1 cells, like Actb, Ctsz, Hmox1, Hspa5, and Vim, as alternatives to viral promoters for producing recombinant proteins, aiming to reduce cellular stress and epigenetic silencing.
  • Researchers identified these promoters through RNAseq data and tested their activity in silico, finding most had equal or better transcriptional activity compared to the commonly used CMV promoter, with some remaining stable even when culture conditions were altered.
  • These endogenous promoters show promise for long-term, high-level gene expression in mammalian cell culture, making them useful for industrial protein production, cell engineering, and synthetic promoter design.]
Read the full abstract

Top posts

Most liked on X and Bluesky
bioRxiv
@biorxivpreprint · X

Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/ITKC2lEZ1M #bioRxiv

Oct 14, 2021 · 8 likes · 3 shares · 138,421 followers View post →
bioRxiv Cell Biology
@biorxiv_cellbio · X

Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/r6poxerdC1 #biorxiv_cellbio

Oct 14, 2021 · 1 like · 1 share · 7,774 followers View post →
2438@ROM
@t2438 · X

RT @biorxivpreprint: Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/…

Oct 14, 2021 · 3 shares · 817 followers View post →
Brian Dean
@bdean_ · X

RT @biorxivpreprint: Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/…

Oct 14, 2021 · 3 shares · 572 followers View post →
Lorenzo Lafranchi
@LoreLafra · X

RT @biorxiv_cellbio: Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/…

Oct 14, 2021 · 1 share · 142 followers View post →
Engineering Papers
@eng_papers · X

Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/RMiykKcie7

Oct 14, 2021 · 44 followers View post →

All mentions

Lorenzo Lafranchi
@LoreLafra · X

RT @biorxiv_cellbio: Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/…

Oct 14, 2021 · 1 share · 142 followers View post →
Engineering Papers
@eng_papers · X

Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/RMiykKcie7

Oct 14, 2021 · 44 followers View post →
Victor
@JeffreyLeyton · X

RT @biorxivpreprint: Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/…

Oct 14, 2021 · 3 shares · 34 followers View post →
Brian Dean
@bdean_ · X

RT @biorxivpreprint: Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/…

Oct 14, 2021 · 3 shares · 572 followers View post →
2438@ROM
@t2438 · X

RT @biorxivpreprint: Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/…

Oct 14, 2021 · 3 shares · 817 followers View post →
bioRxiv
@biorxivpreprint · X

Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/ITKC2lEZ1M #bioRxiv

Oct 14, 2021 · 8 likes · 3 shares · 138,421 followers View post →
bioRxiv Cell Biology
@biorxiv_cellbio · X

Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures https://t.co/r6poxerdC1 #biorxiv_cellbio

Oct 14, 2021 · 1 like · 1 share · 7,774 followers View post →
Optimizing Expression Vectors: Biotherapeutic Protein Production
News

Plasmid 119–120, 2022: 102620; https://doi.org/10.1016/j.plasmid.2022.102620. 33 Running Deer J, Allison DS. High-Level Expression of ...

Dec 19, 2025 View →