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Nat Methods May 2022 PMID 35577958

Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models.

Mark Bates, Jan Keller-Findeisen, Adrian Przybylski, Andreas Hüper, Till Stephan, Peter Ilgen, Angel R Cereceda Delgado, Elisa D'Este, Alexander Egner, Stefan Jakobs, Steffen J Sahl, Stefan W Hell

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Mentions

37
Peak: 22 in Oct 2021
across 3 sources since Oct 2021

Extended reach

186,323
potential audience

Total likes

144

Video views

563

Mentions per month

Oct 2021 – Dec 2023

Where it's discussed

  • X / Twitter32 posts
  • YouTube3 videos
  • Facebook2 posts

AI synopsis

What people are saying

The discussions express strong enthusiasm for the article's technical advancements, with posts describing the impressive imaging of mitochondria and modeling techniques, such as 'incredible complexity embedded inside elegance and simplicity.' The tone underscores admiration for the work's scientific beauty and potential, using words like 'beautiful,' 'amazing,' and 'incredible,' which convey both appreciation and recognition of its significance in expanding microscopy capabilities.

AgreementStrong agreement

Many discussions praise the publication as innovative and impactful, with phrases like 'incredible complexity inside elegance and simplicity' and 'latest work in 3D super-resolution microscopy,' indicating strong alignment with the research's significance

InterestHigh interest

Posts demonstrate high curiosity, sharing links, videos, and emphasizing technological advances, reflected in expressions like 'such a beautiful work' and referencing the detailed imaging techniques used.

EngagementHigh engagement

Participants actively engage with the methodology and implications, mentioning specific imaging results, techniques like spline PSF models, and applications in cellular structures, showing deep interest and understanding.

ImpactModerate impact

While some posts highlight the potential for advancing biological imaging, the overall perception leans toward a recognition of its importance, though not explicitly claiming it will revolutionize the field.

Abstract in brief

  • The study introduces a new 4PiSTORM microscopy technique that achieves high precision 3D imaging by dynamically modeling the point spread function (PSF) with spline-based methods, simplifying the optical setup.
  • This approach accurately captures the temporal changes in the PSF, allowing the system to reach theoretical limits of localization precision (~2.3nm) and reduce artifacts like phase wrapping, thereby enhancing image quality.
  • The improved 4PiSTORM technique was demonstrated by successfully imaging protein and nucleic acid structures in primary neurons and mitochondria, showcasing its potential for detailed biological investigations.]
Read the full abstract

Top posts

Most liked on X and Bluesky
Mark Bates
@wmarkbates · X

Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 19, 2021 · 83 likes · 19 shares · 862 followers View post →
JakobsLab
@JakobsLab · X

We just uploaded new videos to our YouTube Channel: Data from 4Pi STORM imaging of the MICOS subunit Mic60 in a COS-7 cell ... (1/3) https://t.co/Fzs0absE6Z

Jun 3, 2022 · 13 likes · 2,710 followers View post →
Till Stephan
@stephan_till · X

One of my favorite 3D SMLM recordings of mitochondria is now on Youtube. https://t.co/qZyLdddqyi https://t.co/WZ0PFeLuMO https://t.co/JiudEB3Yr5

Jun 5, 2022 · 12 likes · 1,657 followers View post →
JakobsLab
@JakobsLab · X

We have shown you our #SpectrinWaltz with the data from our latest publication (Bates et al., 2022) already here on Twitter...but NOW you can watch it in all its glory (up to 4k) on our YouTube channel

May 23, 2022 · 7 likes · 1 share · 2,710 followers View post →
Till Stephan
@stephan_till · X

The 4Pi-STORM by @wmarkbates flies

Dec 14, 2023 · 7 likes · 1,657 followers View post →
JakobsLab
@JakobsLab · X

... and in a U-2 OS cell. It is always amazing to see how much the distribution of the same protein can differ between different cell lines. Another example of how versatile mitochondria really are!

Jun 3, 2022 · 5 likes · 2,710 followers View post →

All mentions

Till Stephan
@stephan_till · X

The 4Pi-STORM by @wmarkbates flies

Dec 14, 2023 · 7 likes · 1,657 followers View post →
Till Stephan
@stephan_till · X

One of my favorite 3D SMLM recordings of mitochondria is now on Youtube. https://t.co/qZyLdddqyi https://t.co/WZ0PFeLuMO https://t.co/JiudEB3Yr5

Jun 5, 2022 · 12 likes · 1,657 followers View post →
JakobsLab
@JakobsLab · X

... and in a U-2 OS cell. It is always amazing to see how much the distribution of the same protein can differ between different cell lines. Another example of how versatile mitochondria really are!

Jun 3, 2022 · 5 likes · 2,710 followers View post →
JakobsLab
@JakobsLab · X

We just uploaded new videos to our YouTube Channel: Data from 4Pi STORM imaging of the MICOS subunit Mic60 in a COS-7 cell ... (1/3) https://t.co/Fzs0absE6Z

Jun 3, 2022 · 13 likes · 2,710 followers View post →
Peter I.
@pitvandyck · X

RT @JakobsLab: We have shown you our #SpectrinWaltz with the data from our latest publication (Bates et al., 2022) already here on Twitter.…

May 23, 2022 · 1 share · 132 followers View post →
JakobsLab
@JakobsLab · X

We have shown you our #SpectrinWaltz with the data from our latest publication (Bates et al., 2022) already here on Twitter...but NOW you can watch it in all its glory (up to 4k) on our YouTube channel

May 23, 2022 · 7 likes · 1 share · 2,710 followers View post →
DZ Gong | 龚 | ゴン | 공
@dz_gong · X

Paper read today #83 from @StefanHellLabs: Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models https://t.co/ELV3Ex4acZ Such an beautiful work. Incredible complexity embedded inside elegance and simplicity. https://t.co/8dqOpx3Yw3

May 18, 2022 · 621 followers View post →
Nano IJ
@nanomicroscopy · X

Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models https://t.co/S5gFdq5kpL https://t.co/ErDbIrERDv

May 17, 2022 · 2 likes · 594 followers View post →
Super Res Microscopy
@SRMicro_papers · X

Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models by Mark Bates https://t.co/s0lThcpNQX

May 17, 2022 · 3,125 followers View post →
Helena Paris
@Educators_Link · X

Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models https://t.co/avrs9Hh0F0

May 16, 2022 · 4 followers View post →
@Katonalab
@katonalab · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 26, 2021 · 19 shares · 293 followers View post →
Abbas Rizvi
@realAbbasRizvi · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 25, 2021 · 19 shares · 96 followers View post →
Brian English
@sciencethecat · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 25, 2021 · 19 shares · 2,691 followers View post →
PromisingPreprints
@PromPreprint · X

Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models https://t.co/iCe1htlCq1

Oct 25, 2021 · 3,312 followers View post →
Optical Nanoscopy
@OptNanoscopy · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 24, 2021 · 19 shares · 3,282 followers View post →
Alf Honigmann
@Honigmann_Lab · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 24, 2021 · 19 shares · 1,887 followers View post →
Chien-Hua Tu 杜建樺
@ChienHuaTu1 · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 20, 2021 · 19 shares · 111 followers View post →
Vicidomini Lab
@VicidominiLab · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 20, 2021 · 19 shares · 1,894 followers View post →
Roman Tsukanov
@RomanTsukanov · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 20, 2021 · 19 shares · 476 followers View post →
Ilaria Testa
@IlariaTesta4 · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 20, 2021 · 19 shares · 2,728 followers View post →
JakobsLab
@JakobsLab · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 20, 2021 · 19 shares · 2,708 followers View post →
Peter I.
@pitvandyck · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 20, 2021 · 19 shares · 131 followers View post →
biophotonicat
@biophotonicat · X

Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models (relevance: 75%) https://t.co/5mpXSWWEOc #biophotonics #biomedicaloptics https://t.co/VotsvVXpsS

Oct 19, 2021 · 1,529 followers View post →
Sam Peng
@CTSPeng · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 19, 2021 · 19 shares · 636 followers View post →
Tino Pleiner
@pleinerlab · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 19, 2021 · 19 shares · 994 followers View post →
Amit Koikkarah Aji
@MalluKidAtYale · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 19, 2021 · 19 shares · 302 followers View post →
Francisco Balzarotti
@BalzarottiFran · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 19, 2021 · 19 shares · 1,526 followers View post →
Benjamin Compans
@bcompans · X

RT @wmarkbates: Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 19, 2021 · 19 shares · 871 followers View post →
Nano IJ
@nanomicroscopy · X

Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models https://t.co/b7jWQzC1A2

Oct 19, 2021 · 1 like · 594 followers View post →
Mark Bates
@wmarkbates · X

Happy to report our latest work in 3D super-resolution fluorescence microscopy with interferometric detection

Oct 19, 2021 · 83 likes · 19 shares · 862 followers View post →
bioRxiv Biophysics
@biorxiv_biophys · X

Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models https://t.co/bKrwExO2d2 #biorxiv_biophys

Oct 19, 2021 · 5,370 followers View post →
bioRxiv
@biorxivpreprint · X

Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models https://t.co/vBE1zZIwV5 #bioRxiv

Oct 19, 2021 · 136,898 followers View post →